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Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite

To design a new electrochemical horseradish peroxidase (HRP) biosensor with excellent analytical performance, black phosphorene (BP) nanosheets and single-walled carbon nanotubes (SWCNTs) nanocomposites were used as the modifier, with a carbon ionic liquid electrode (CILE) as the substrate electrode...

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Autores principales: Li, Xiaoqing, Wang, Lisi, Wang, Baoli, Zhang, Siyue, Jiang, Meng, Fu, Wanting, Sun, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694212/
https://www.ncbi.nlm.nih.gov/pubmed/36432164
http://dx.doi.org/10.3390/molecules27228064
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author Li, Xiaoqing
Wang, Lisi
Wang, Baoli
Zhang, Siyue
Jiang, Meng
Fu, Wanting
Sun, Wei
author_facet Li, Xiaoqing
Wang, Lisi
Wang, Baoli
Zhang, Siyue
Jiang, Meng
Fu, Wanting
Sun, Wei
author_sort Li, Xiaoqing
collection PubMed
description To design a new electrochemical horseradish peroxidase (HRP) biosensor with excellent analytical performance, black phosphorene (BP) nanosheets and single-walled carbon nanotubes (SWCNTs) nanocomposites were used as the modifier, with a carbon ionic liquid electrode (CILE) as the substrate electrode. The SWCNTs-BP nanocomposite was synthesized by a simple in situ mixing procedure and modified on the CILE surface by the direct casting method. Then HRP was immobilized on the modified electrode with Nafion film. The electrocatalysis of this electrochemical HRP biosensor to various targets was further explored. Experimental results indicated that the direct electrochemistry of HRP was realized with a pair of symmetric and quasi-reversible redox peaks appeared, which was due to the presence of SWCNTs-BP on the surface of CILE, exhibiting synergistic effects with high electrical conductivity and good biocompatibility. Excellent electrocatalytic activity to trichloroacetic acid (TCA), sodium nitrite (NaNO(2)), and hydrogen peroxide (H(2)O(2)) were realized, with a wide linear range and a low detection limit. Different real samples, such as a medical facial peel solution, the soak water of pickled vegetables, and a 3% H(2)O(2) disinfectant, were further analyzed, with satisfactory results, further proving the potential practical applications for the electrochemical biosensor.
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spelling pubmed-96942122022-11-26 Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite Li, Xiaoqing Wang, Lisi Wang, Baoli Zhang, Siyue Jiang, Meng Fu, Wanting Sun, Wei Molecules Article To design a new electrochemical horseradish peroxidase (HRP) biosensor with excellent analytical performance, black phosphorene (BP) nanosheets and single-walled carbon nanotubes (SWCNTs) nanocomposites were used as the modifier, with a carbon ionic liquid electrode (CILE) as the substrate electrode. The SWCNTs-BP nanocomposite was synthesized by a simple in situ mixing procedure and modified on the CILE surface by the direct casting method. Then HRP was immobilized on the modified electrode with Nafion film. The electrocatalysis of this electrochemical HRP biosensor to various targets was further explored. Experimental results indicated that the direct electrochemistry of HRP was realized with a pair of symmetric and quasi-reversible redox peaks appeared, which was due to the presence of SWCNTs-BP on the surface of CILE, exhibiting synergistic effects with high electrical conductivity and good biocompatibility. Excellent electrocatalytic activity to trichloroacetic acid (TCA), sodium nitrite (NaNO(2)), and hydrogen peroxide (H(2)O(2)) were realized, with a wide linear range and a low detection limit. Different real samples, such as a medical facial peel solution, the soak water of pickled vegetables, and a 3% H(2)O(2) disinfectant, were further analyzed, with satisfactory results, further proving the potential practical applications for the electrochemical biosensor. MDPI 2022-11-20 /pmc/articles/PMC9694212/ /pubmed/36432164 http://dx.doi.org/10.3390/molecules27228064 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Xiaoqing
Wang, Lisi
Wang, Baoli
Zhang, Siyue
Jiang, Meng
Fu, Wanting
Sun, Wei
Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite
title Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite
title_full Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite
title_fullStr Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite
title_full_unstemmed Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite
title_short Preparation and Application of Electrochemical Horseradish Peroxidase Sensor Based on a Black Phosphorene and Single-Walled Carbon Nanotubes Nanocomposite
title_sort preparation and application of electrochemical horseradish peroxidase sensor based on a black phosphorene and single-walled carbon nanotubes nanocomposite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694212/
https://www.ncbi.nlm.nih.gov/pubmed/36432164
http://dx.doi.org/10.3390/molecules27228064
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