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Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice

Fertilization is a complex process that requires successive stages and culminates in the adhesion/fusion of gamete membranes. If the question of the involvement of oocyte integrins has been swept away by deletion experiments, that of the involvement of sperm integrins remains to be further character...

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Autores principales: Mimouni, Nour El Houda, Ialy-Radio, Côme, Denizot, Anne-Lyse, Lagoutte, Isabelle, Frolikova, Michaela, Komrskova, Katerina, Barbaux, Sandrine, Ziyyat, Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694253/
https://www.ncbi.nlm.nih.gov/pubmed/36430291
http://dx.doi.org/10.3390/ijms232213812
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author Mimouni, Nour El Houda
Ialy-Radio, Côme
Denizot, Anne-Lyse
Lagoutte, Isabelle
Frolikova, Michaela
Komrskova, Katerina
Barbaux, Sandrine
Ziyyat, Ahmed
author_facet Mimouni, Nour El Houda
Ialy-Radio, Côme
Denizot, Anne-Lyse
Lagoutte, Isabelle
Frolikova, Michaela
Komrskova, Katerina
Barbaux, Sandrine
Ziyyat, Ahmed
author_sort Mimouni, Nour El Houda
collection PubMed
description Fertilization is a complex process that requires successive stages and culminates in the adhesion/fusion of gamete membranes. If the question of the involvement of oocyte integrins has been swept away by deletion experiments, that of the involvement of sperm integrins remains to be further characterized. In the present study, we addressed the question of the feasibility of sperm–oocyte adhesion/fusion and early implantation in the absence of sperm β1 integrin. Males and females with β1 integrin-depleted sperm and oocytes were mated, and fertilization outcome was monitored by a gestational ultrasound analysis. Results suggest that although the sperm β1 integrin participates in gamete adhesion/fusion, it is dispensable for fertilization in mice. However, sperm- and/or oocyte-originated integrin β1 is essential for post-implantation development. Redundancy phenomena could be at the origin of a compensatory expression or alternative dimerization pattern.
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spelling pubmed-96942532022-11-26 Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice Mimouni, Nour El Houda Ialy-Radio, Côme Denizot, Anne-Lyse Lagoutte, Isabelle Frolikova, Michaela Komrskova, Katerina Barbaux, Sandrine Ziyyat, Ahmed Int J Mol Sci Article Fertilization is a complex process that requires successive stages and culminates in the adhesion/fusion of gamete membranes. If the question of the involvement of oocyte integrins has been swept away by deletion experiments, that of the involvement of sperm integrins remains to be further characterized. In the present study, we addressed the question of the feasibility of sperm–oocyte adhesion/fusion and early implantation in the absence of sperm β1 integrin. Males and females with β1 integrin-depleted sperm and oocytes were mated, and fertilization outcome was monitored by a gestational ultrasound analysis. Results suggest that although the sperm β1 integrin participates in gamete adhesion/fusion, it is dispensable for fertilization in mice. However, sperm- and/or oocyte-originated integrin β1 is essential for post-implantation development. Redundancy phenomena could be at the origin of a compensatory expression or alternative dimerization pattern. MDPI 2022-11-09 /pmc/articles/PMC9694253/ /pubmed/36430291 http://dx.doi.org/10.3390/ijms232213812 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mimouni, Nour El Houda
Ialy-Radio, Côme
Denizot, Anne-Lyse
Lagoutte, Isabelle
Frolikova, Michaela
Komrskova, Katerina
Barbaux, Sandrine
Ziyyat, Ahmed
Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
title Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
title_full Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
title_fullStr Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
title_full_unstemmed Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
title_short Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
title_sort fertilization, but not post-implantation development, can occur in the absence of sperm and oocyte beta1 integrin in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694253/
https://www.ncbi.nlm.nih.gov/pubmed/36430291
http://dx.doi.org/10.3390/ijms232213812
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