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Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health

Lactobacilli with probiotic properties have emerged as promising tools for both the prevention and treatment of vaginal dysbiosis. The present study aimed to study the in vitro probiotic potential of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain isolated from a healthy vaginal ecosystem....

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Autores principales: Pino, Alessandra, Vaccalluzzo, Amanda, Caggia, Cinzia, Balzaretti, Silvia, Vanella, Luca, Sorrenti, Valeria, Ronkainen, Aki, Satokari, Reetta, Randazzo, Cinzia Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694283/
https://www.ncbi.nlm.nih.gov/pubmed/36432588
http://dx.doi.org/10.3390/nu14224902
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author Pino, Alessandra
Vaccalluzzo, Amanda
Caggia, Cinzia
Balzaretti, Silvia
Vanella, Luca
Sorrenti, Valeria
Ronkainen, Aki
Satokari, Reetta
Randazzo, Cinzia Lucia
author_facet Pino, Alessandra
Vaccalluzzo, Amanda
Caggia, Cinzia
Balzaretti, Silvia
Vanella, Luca
Sorrenti, Valeria
Ronkainen, Aki
Satokari, Reetta
Randazzo, Cinzia Lucia
author_sort Pino, Alessandra
collection PubMed
description Lactobacilli with probiotic properties have emerged as promising tools for both the prevention and treatment of vaginal dysbiosis. The present study aimed to study the in vitro probiotic potential of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain isolated from a healthy vaginal ecosystem. The strain was evaluated for both functional (antagonistic activity against pathogens; H(2)O(2), organic acid, and lactic acid production; antioxidant and anti-inflammatory activities; ability to adhere to intestinal mucus and to both CaCo-2 and VK7/E6E7 cell lines; exopolysaccharide production; surface properties; and ability to survive during gastrointestinal transit) and safety (hemolytic, DNase, and gelatinase activities; mucin degradation ability; production of biogenic amines; and resistance to antimicrobials) characteristics. Data revealed that the tested strain was able to antagonize a broad spectrum of vaginal pathogens. In addition, the adhesion capacity to both vaginal and intestinal cell lines, as well as anti-inflammatory and antioxidant activities, was detected. The ability of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain to survive under harsh environmental conditions occurring during the gastrointestinal passage suggests its possible oral delivery. Thus, in vitro data highlighted interesting probiotic properties of the CA15 (DSM 33960) strain, which could represent a valuable candidate for in vivo vaginal infections treatment.
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spelling pubmed-96942832022-11-26 Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health Pino, Alessandra Vaccalluzzo, Amanda Caggia, Cinzia Balzaretti, Silvia Vanella, Luca Sorrenti, Valeria Ronkainen, Aki Satokari, Reetta Randazzo, Cinzia Lucia Nutrients Article Lactobacilli with probiotic properties have emerged as promising tools for both the prevention and treatment of vaginal dysbiosis. The present study aimed to study the in vitro probiotic potential of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain isolated from a healthy vaginal ecosystem. The strain was evaluated for both functional (antagonistic activity against pathogens; H(2)O(2), organic acid, and lactic acid production; antioxidant and anti-inflammatory activities; ability to adhere to intestinal mucus and to both CaCo-2 and VK7/E6E7 cell lines; exopolysaccharide production; surface properties; and ability to survive during gastrointestinal transit) and safety (hemolytic, DNase, and gelatinase activities; mucin degradation ability; production of biogenic amines; and resistance to antimicrobials) characteristics. Data revealed that the tested strain was able to antagonize a broad spectrum of vaginal pathogens. In addition, the adhesion capacity to both vaginal and intestinal cell lines, as well as anti-inflammatory and antioxidant activities, was detected. The ability of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain to survive under harsh environmental conditions occurring during the gastrointestinal passage suggests its possible oral delivery. Thus, in vitro data highlighted interesting probiotic properties of the CA15 (DSM 33960) strain, which could represent a valuable candidate for in vivo vaginal infections treatment. MDPI 2022-11-19 /pmc/articles/PMC9694283/ /pubmed/36432588 http://dx.doi.org/10.3390/nu14224902 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pino, Alessandra
Vaccalluzzo, Amanda
Caggia, Cinzia
Balzaretti, Silvia
Vanella, Luca
Sorrenti, Valeria
Ronkainen, Aki
Satokari, Reetta
Randazzo, Cinzia Lucia
Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health
title Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health
title_full Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health
title_fullStr Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health
title_full_unstemmed Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health
title_short Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health
title_sort lacticaseibacillus rhamnosus ca15 (dsm 33960) as a candidate probiotic strain for human health
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694283/
https://www.ncbi.nlm.nih.gov/pubmed/36432588
http://dx.doi.org/10.3390/nu14224902
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