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In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.

Fungal infections have increased in recent decades with considerable morbidity and mortality, mainly in immunosuppressed or admitted-to-the-ICU patients. The fungal resistance to conventional antifungal treatments has become a public health problem, especially with Candida that presents resistance t...

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Autores principales: Pinilla, Gladys, Coronado, Yenifer Tatiana, Chaves, Gabriel, Muñoz, Liliana, Navarrete, Jeannette, Salazar, Luz Mary, Taborda, Carlos Pelleschi, Muñoz, Julián E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694450/
https://www.ncbi.nlm.nih.gov/pubmed/36354940
http://dx.doi.org/10.3390/jof8111173
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author Pinilla, Gladys
Coronado, Yenifer Tatiana
Chaves, Gabriel
Muñoz, Liliana
Navarrete, Jeannette
Salazar, Luz Mary
Taborda, Carlos Pelleschi
Muñoz, Julián E.
author_facet Pinilla, Gladys
Coronado, Yenifer Tatiana
Chaves, Gabriel
Muñoz, Liliana
Navarrete, Jeannette
Salazar, Luz Mary
Taborda, Carlos Pelleschi
Muñoz, Julián E.
author_sort Pinilla, Gladys
collection PubMed
description Fungal infections have increased in recent decades with considerable morbidity and mortality, mainly in immunosuppressed or admitted-to-the-ICU patients. The fungal resistance to conventional antifungal treatments has become a public health problem, especially with Candida that presents resistance to several antifungals. Therefore, generating new alternatives of antifungal therapy is fundamental. One of these possibilities is the use of antimicrobial peptides, such as LL-37, which acts on the disruption of the microorganism membrane and promotes immunomodulatory effects in the host. In this study, we evaluated the in vitro antifungal activity of the LL-37 analogue peptides (AC-1, LL37-1, AC-2, and D) against different Candida spp. and clinical isolates obtained from patients with vulvovaginal candidiasis. Our results suggest that the peptides with the best ranges of MICs were LL37-1 and AC-2 (0.07 µM) against the strains studied. This inhibitory effect was confirmed by analyzing the yeast growth curves that evidenced a significant decrease in the fungal growth after exposure to LL-37 peptides. By the XTT technique we observed a significant reduction in the biofilm formation process when compared to yeasts untreated with the analogue peptides. In conclusion, we suggest that LL-37 analogue peptides may play an important antimicrobial role against Candida spp.
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spelling pubmed-96944502022-11-26 In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp. Pinilla, Gladys Coronado, Yenifer Tatiana Chaves, Gabriel Muñoz, Liliana Navarrete, Jeannette Salazar, Luz Mary Taborda, Carlos Pelleschi Muñoz, Julián E. J Fungi (Basel) Article Fungal infections have increased in recent decades with considerable morbidity and mortality, mainly in immunosuppressed or admitted-to-the-ICU patients. The fungal resistance to conventional antifungal treatments has become a public health problem, especially with Candida that presents resistance to several antifungals. Therefore, generating new alternatives of antifungal therapy is fundamental. One of these possibilities is the use of antimicrobial peptides, such as LL-37, which acts on the disruption of the microorganism membrane and promotes immunomodulatory effects in the host. In this study, we evaluated the in vitro antifungal activity of the LL-37 analogue peptides (AC-1, LL37-1, AC-2, and D) against different Candida spp. and clinical isolates obtained from patients with vulvovaginal candidiasis. Our results suggest that the peptides with the best ranges of MICs were LL37-1 and AC-2 (0.07 µM) against the strains studied. This inhibitory effect was confirmed by analyzing the yeast growth curves that evidenced a significant decrease in the fungal growth after exposure to LL-37 peptides. By the XTT technique we observed a significant reduction in the biofilm formation process when compared to yeasts untreated with the analogue peptides. In conclusion, we suggest that LL-37 analogue peptides may play an important antimicrobial role against Candida spp. MDPI 2022-11-07 /pmc/articles/PMC9694450/ /pubmed/36354940 http://dx.doi.org/10.3390/jof8111173 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pinilla, Gladys
Coronado, Yenifer Tatiana
Chaves, Gabriel
Muñoz, Liliana
Navarrete, Jeannette
Salazar, Luz Mary
Taborda, Carlos Pelleschi
Muñoz, Julián E.
In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.
title In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.
title_full In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.
title_fullStr In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.
title_full_unstemmed In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.
title_short In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.
title_sort in vitro antifungal activity of ll-37 analogue peptides against candida spp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694450/
https://www.ncbi.nlm.nih.gov/pubmed/36354940
http://dx.doi.org/10.3390/jof8111173
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