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Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex...

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Autores principales: Watanabe, Chisato, Shibuya, Hirotoshi, Ichiyama, Yusuke, Okamura, Eiichi, Tsukiyama-Fujii, Setsuko, Tsukiyama, Tomoyuki, Matsumoto, Shoma, Matsushita, Jun, Azami, Takuya, Kubota, Yoshiaki, Ohji, Masahito, Sugiyama, Fumihiro, Takahashi, Satoru, Mizuno, Seiya, Tamura, Masaru, Mizutani, Ken-ichi, Ema, Masatsugu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694714/
https://www.ncbi.nlm.nih.gov/pubmed/36362885
http://dx.doi.org/10.3390/life12111730
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author Watanabe, Chisato
Shibuya, Hirotoshi
Ichiyama, Yusuke
Okamura, Eiichi
Tsukiyama-Fujii, Setsuko
Tsukiyama, Tomoyuki
Matsumoto, Shoma
Matsushita, Jun
Azami, Takuya
Kubota, Yoshiaki
Ohji, Masahito
Sugiyama, Fumihiro
Takahashi, Satoru
Mizuno, Seiya
Tamura, Masaru
Mizutani, Ken-ichi
Ema, Masatsugu
author_facet Watanabe, Chisato
Shibuya, Hirotoshi
Ichiyama, Yusuke
Okamura, Eiichi
Tsukiyama-Fujii, Setsuko
Tsukiyama, Tomoyuki
Matsumoto, Shoma
Matsushita, Jun
Azami, Takuya
Kubota, Yoshiaki
Ohji, Masahito
Sugiyama, Fumihiro
Takahashi, Satoru
Mizuno, Seiya
Tamura, Masaru
Mizutani, Ken-ichi
Ema, Masatsugu
author_sort Watanabe, Chisato
collection PubMed
description Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice.
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spelling pubmed-96947142022-11-26 Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice Watanabe, Chisato Shibuya, Hirotoshi Ichiyama, Yusuke Okamura, Eiichi Tsukiyama-Fujii, Setsuko Tsukiyama, Tomoyuki Matsumoto, Shoma Matsushita, Jun Azami, Takuya Kubota, Yoshiaki Ohji, Masahito Sugiyama, Fumihiro Takahashi, Satoru Mizuno, Seiya Tamura, Masaru Mizutani, Ken-ichi Ema, Masatsugu Life (Basel) Article Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice. MDPI 2022-10-28 /pmc/articles/PMC9694714/ /pubmed/36362885 http://dx.doi.org/10.3390/life12111730 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Watanabe, Chisato
Shibuya, Hirotoshi
Ichiyama, Yusuke
Okamura, Eiichi
Tsukiyama-Fujii, Setsuko
Tsukiyama, Tomoyuki
Matsumoto, Shoma
Matsushita, Jun
Azami, Takuya
Kubota, Yoshiaki
Ohji, Masahito
Sugiyama, Fumihiro
Takahashi, Satoru
Mizuno, Seiya
Tamura, Masaru
Mizutani, Ken-ichi
Ema, Masatsugu
Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
title Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
title_full Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
title_fullStr Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
title_full_unstemmed Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
title_short Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
title_sort essential roles of exocyst complex component 3-like 2 on cardiovascular development in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694714/
https://www.ncbi.nlm.nih.gov/pubmed/36362885
http://dx.doi.org/10.3390/life12111730
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