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Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice
Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694714/ https://www.ncbi.nlm.nih.gov/pubmed/36362885 http://dx.doi.org/10.3390/life12111730 |
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author | Watanabe, Chisato Shibuya, Hirotoshi Ichiyama, Yusuke Okamura, Eiichi Tsukiyama-Fujii, Setsuko Tsukiyama, Tomoyuki Matsumoto, Shoma Matsushita, Jun Azami, Takuya Kubota, Yoshiaki Ohji, Masahito Sugiyama, Fumihiro Takahashi, Satoru Mizuno, Seiya Tamura, Masaru Mizutani, Ken-ichi Ema, Masatsugu |
author_facet | Watanabe, Chisato Shibuya, Hirotoshi Ichiyama, Yusuke Okamura, Eiichi Tsukiyama-Fujii, Setsuko Tsukiyama, Tomoyuki Matsumoto, Shoma Matsushita, Jun Azami, Takuya Kubota, Yoshiaki Ohji, Masahito Sugiyama, Fumihiro Takahashi, Satoru Mizuno, Seiya Tamura, Masaru Mizutani, Ken-ichi Ema, Masatsugu |
author_sort | Watanabe, Chisato |
collection | PubMed |
description | Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice. |
format | Online Article Text |
id | pubmed-9694714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96947142022-11-26 Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice Watanabe, Chisato Shibuya, Hirotoshi Ichiyama, Yusuke Okamura, Eiichi Tsukiyama-Fujii, Setsuko Tsukiyama, Tomoyuki Matsumoto, Shoma Matsushita, Jun Azami, Takuya Kubota, Yoshiaki Ohji, Masahito Sugiyama, Fumihiro Takahashi, Satoru Mizuno, Seiya Tamura, Masaru Mizutani, Ken-ichi Ema, Masatsugu Life (Basel) Article Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of exocyst complex component (Exoc) 3-like 2 (Exoc3l2) during development in mice. We found that Exoc3l1, Exoc3l2, Exoc3l3 and Exoc3l4 are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of Exoc3l2 knock-out (KO) mice showed that disruption of Exoc3l2 resulted in lethal in utero. Substantial numbers of Exoc3l2 KO embryos exhibited hemorrhaging. Deletion of Exoc3l2 using Tie2-Cre transgenic mice demonstrated that Exoc3l2 in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that Exoc3l2 is essential for cardiovascular and brain development in mice. MDPI 2022-10-28 /pmc/articles/PMC9694714/ /pubmed/36362885 http://dx.doi.org/10.3390/life12111730 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Watanabe, Chisato Shibuya, Hirotoshi Ichiyama, Yusuke Okamura, Eiichi Tsukiyama-Fujii, Setsuko Tsukiyama, Tomoyuki Matsumoto, Shoma Matsushita, Jun Azami, Takuya Kubota, Yoshiaki Ohji, Masahito Sugiyama, Fumihiro Takahashi, Satoru Mizuno, Seiya Tamura, Masaru Mizutani, Ken-ichi Ema, Masatsugu Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice |
title | Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice |
title_full | Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice |
title_fullStr | Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice |
title_full_unstemmed | Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice |
title_short | Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice |
title_sort | essential roles of exocyst complex component 3-like 2 on cardiovascular development in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694714/ https://www.ncbi.nlm.nih.gov/pubmed/36362885 http://dx.doi.org/10.3390/life12111730 |
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