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Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma

Brown rot caused by the pathogen of the genus Monilinia is the most destructive disease in peaches worldwide. It has seriously reduced the economic value of the peach (Prunus persica (L.) Batsch) in Nyingchi and Qamdo, Tibet, China. Monilinia fructicola, Monilia mumecola, and M. yunnanensis have bee...

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Autores principales: Zhang, Shuwu, Xiang, Dong, Sun, Chenxi, Han, Kaidi, Li, Tong, Zhou, Jingjiang, Xu, Bingliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694890/
https://www.ncbi.nlm.nih.gov/pubmed/36354941
http://dx.doi.org/10.3390/jof8111174
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author Zhang, Shuwu
Xiang, Dong
Sun, Chenxi
Han, Kaidi
Li, Tong
Zhou, Jingjiang
Xu, Bingliang
author_facet Zhang, Shuwu
Xiang, Dong
Sun, Chenxi
Han, Kaidi
Li, Tong
Zhou, Jingjiang
Xu, Bingliang
author_sort Zhang, Shuwu
collection PubMed
description Brown rot caused by the pathogen of the genus Monilinia is the most destructive disease in peaches worldwide. It has seriously reduced the economic value of the peach (Prunus persica (L.) Batsch) in Nyingchi and Qamdo, Tibet, China. Monilinia fructicola, Monilia mumecola, and M. yunnanensis have been reported as the causal agents of brown rot disease on stone fruits in China. In this study, we report on the identification of M. yunnanensis in peach orchards in Nyingchi and Qamdo, Tibet. From twenty-three isolates with the same characteristics, we identified the representative single-spore isolates T8-1, T8-8, and T8-20 as M. yunnanensis and confirmed that the Tibet brown rot disease was caused by M. yunnanensis based on the morphological characteristics and molecular analyses. The phylogenetic analysis of the glyceraldehyde-3-phosphate dehydrogenase (G3PDH) and β-tubulin (TUB2) nucleotide sequences and the multiplex PCR identification revealed that the representative isolates T8-1, T8-8, and T8-20 were more closely related to M. yunnanensis than other Monilinia species. Furthermore, the biocontrol strain of Trichoderma T6 presented significant antagonistic activity on the M. yunnanensis T8-1 isolate (T8-1) among the five Trichoderma strains. The highest inhibitory rates for Trichoderma T6 and its fermentation product against T8-1 mycelial growth were 72.13% and 68.25%, respectively. The obvious inhibition zone displayed on the colony interaction area between the colony of T8-1 isolate and Trichoderma T6 and the morphological characterization of the T8-1 hyphae were enlarged and malformed after inoculation with the Trichoderma T6 fermentation product at 20-fold dilution. Our results indicate that the strain of Trichoderma T6 could be considered as a beneficial biocontrol agent in managing brown rot of peach fruit disease.
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spelling pubmed-96948902022-11-26 Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma Zhang, Shuwu Xiang, Dong Sun, Chenxi Han, Kaidi Li, Tong Zhou, Jingjiang Xu, Bingliang J Fungi (Basel) Article Brown rot caused by the pathogen of the genus Monilinia is the most destructive disease in peaches worldwide. It has seriously reduced the economic value of the peach (Prunus persica (L.) Batsch) in Nyingchi and Qamdo, Tibet, China. Monilinia fructicola, Monilia mumecola, and M. yunnanensis have been reported as the causal agents of brown rot disease on stone fruits in China. In this study, we report on the identification of M. yunnanensis in peach orchards in Nyingchi and Qamdo, Tibet. From twenty-three isolates with the same characteristics, we identified the representative single-spore isolates T8-1, T8-8, and T8-20 as M. yunnanensis and confirmed that the Tibet brown rot disease was caused by M. yunnanensis based on the morphological characteristics and molecular analyses. The phylogenetic analysis of the glyceraldehyde-3-phosphate dehydrogenase (G3PDH) and β-tubulin (TUB2) nucleotide sequences and the multiplex PCR identification revealed that the representative isolates T8-1, T8-8, and T8-20 were more closely related to M. yunnanensis than other Monilinia species. Furthermore, the biocontrol strain of Trichoderma T6 presented significant antagonistic activity on the M. yunnanensis T8-1 isolate (T8-1) among the five Trichoderma strains. The highest inhibitory rates for Trichoderma T6 and its fermentation product against T8-1 mycelial growth were 72.13% and 68.25%, respectively. The obvious inhibition zone displayed on the colony interaction area between the colony of T8-1 isolate and Trichoderma T6 and the morphological characterization of the T8-1 hyphae were enlarged and malformed after inoculation with the Trichoderma T6 fermentation product at 20-fold dilution. Our results indicate that the strain of Trichoderma T6 could be considered as a beneficial biocontrol agent in managing brown rot of peach fruit disease. MDPI 2022-11-08 /pmc/articles/PMC9694890/ /pubmed/36354941 http://dx.doi.org/10.3390/jof8111174 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Shuwu
Xiang, Dong
Sun, Chenxi
Han, Kaidi
Li, Tong
Zhou, Jingjiang
Xu, Bingliang
Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma
title Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma
title_full Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma
title_fullStr Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma
title_full_unstemmed Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma
title_short Morphological and Molecular Identification of Peach Brown Rot Disease in Tibet and Exploration of the Biocontrol Efficiency of Trichoderma
title_sort morphological and molecular identification of peach brown rot disease in tibet and exploration of the biocontrol efficiency of trichoderma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694890/
https://www.ncbi.nlm.nih.gov/pubmed/36354941
http://dx.doi.org/10.3390/jof8111174
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