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Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis

Objectives: Osteoarthritis (OA) is a chronic degenerative disorder of the joint characterized by cartilage breakdown and synovial inflammation. A number of different cells of innate and adaptive immunity contribute to joint pathology during OA inflammation. The interaction between the local synovial...

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Autores principales: Boneva, Blagovesta, Ralchev, Nikola, Ganova, Petya, Tchorbanov, Andrey, Mihaylova, Nikolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694943/
https://www.ncbi.nlm.nih.gov/pubmed/36431073
http://dx.doi.org/10.3390/life12111938
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author Boneva, Blagovesta
Ralchev, Nikola
Ganova, Petya
Tchorbanov, Andrey
Mihaylova, Nikolina
author_facet Boneva, Blagovesta
Ralchev, Nikola
Ganova, Petya
Tchorbanov, Andrey
Mihaylova, Nikolina
author_sort Boneva, Blagovesta
collection PubMed
description Objectives: Osteoarthritis (OA) is a chronic degenerative disorder of the joint characterized by cartilage breakdown and synovial inflammation. A number of different cells of innate and adaptive immunity contribute to joint pathology during OA inflammation. The interaction between the local synovial and systemic inflammatory cellular response and the structural changes in the joint is still unknown. The objective of this study was to investigate the role of the different types of immune cells in the development of OA. Methods: Collagenase-induced osteoarthritis was induced in Balb/c mice; flow cytometry analysis; and histopathological damages were assessed in histological sections stained with H&E, Toluidine blue, and Safranin O. Results: Flow cytometry analysis showed B lymphocyte infiltration in the active phase of inflammation and an increase in the effector T cell population into the synovium. An increased activation state of cytotoxic T cells and of NK cell populations in the spleen and synovium was also found. The differentiation of NK cells from a cytotoxic phenotype in early OA to cells with an effector phenotype in the chronic phase of the disease followed. Conclusions: A number of different cells contribute to inflammatory processes in OA. The correlation between their phenotype and the inflammatory pathophysiology could result in the development of novel approaches to suppress destructive changes in the joint.
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spelling pubmed-96949432022-11-26 Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis Boneva, Blagovesta Ralchev, Nikola Ganova, Petya Tchorbanov, Andrey Mihaylova, Nikolina Life (Basel) Article Objectives: Osteoarthritis (OA) is a chronic degenerative disorder of the joint characterized by cartilage breakdown and synovial inflammation. A number of different cells of innate and adaptive immunity contribute to joint pathology during OA inflammation. The interaction between the local synovial and systemic inflammatory cellular response and the structural changes in the joint is still unknown. The objective of this study was to investigate the role of the different types of immune cells in the development of OA. Methods: Collagenase-induced osteoarthritis was induced in Balb/c mice; flow cytometry analysis; and histopathological damages were assessed in histological sections stained with H&E, Toluidine blue, and Safranin O. Results: Flow cytometry analysis showed B lymphocyte infiltration in the active phase of inflammation and an increase in the effector T cell population into the synovium. An increased activation state of cytotoxic T cells and of NK cell populations in the spleen and synovium was also found. The differentiation of NK cells from a cytotoxic phenotype in early OA to cells with an effector phenotype in the chronic phase of the disease followed. Conclusions: A number of different cells contribute to inflammatory processes in OA. The correlation between their phenotype and the inflammatory pathophysiology could result in the development of novel approaches to suppress destructive changes in the joint. MDPI 2022-11-21 /pmc/articles/PMC9694943/ /pubmed/36431073 http://dx.doi.org/10.3390/life12111938 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boneva, Blagovesta
Ralchev, Nikola
Ganova, Petya
Tchorbanov, Andrey
Mihaylova, Nikolina
Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis
title Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis
title_full Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis
title_fullStr Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis
title_full_unstemmed Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis
title_short Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis
title_sort collagenase-induced mouse model of osteoarthritis—a thorough flow cytometry analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694943/
https://www.ncbi.nlm.nih.gov/pubmed/36431073
http://dx.doi.org/10.3390/life12111938
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