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The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study

BACKGROUND AND AIMS: Lymphovascular invasion (LVI) is an indicator of lymph node metastasis and poor prognosis in various cancers including squamous cell carcinoma (SCC). Despite being easily resectable and having little potential for LVI; SCC displays aggressive behavior and often results in the de...

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Autores principales: Robison, Shayene, Ngwenya, Sharol, Molaudzi, Mulalo, Molepo, Julitha, Adeola, Henry, Magangane, Pumza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695083/
https://www.ncbi.nlm.nih.gov/pubmed/36447566
http://dx.doi.org/10.1002/hsr2.958
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author Robison, Shayene
Ngwenya, Sharol
Molaudzi, Mulalo
Molepo, Julitha
Adeola, Henry
Magangane, Pumza
author_facet Robison, Shayene
Ngwenya, Sharol
Molaudzi, Mulalo
Molepo, Julitha
Adeola, Henry
Magangane, Pumza
author_sort Robison, Shayene
collection PubMed
description BACKGROUND AND AIMS: Lymphovascular invasion (LVI) is an indicator of lymph node metastasis and poor prognosis in various cancers including squamous cell carcinoma (SCC). Despite being easily resectable and having little potential for LVI; SCC displays aggressive behavior and often results in the death of the patient. With this in mind, it may be useful to investigate the clinical, pathological, and microRNA expression profile associated with LVI in SCC. METHODS: We evaluated the histological hallmarks associated with LVI from 16 formalin fixed paraffin embedded (FFPE) tissue samples (10 LVI−, 6 LVI+). We also quantified the expression of 10 microRNAs (hsa‐miR‐21‐5p, hsa‐miR‐21‐3p, hsa‐miR‐155‐5p, hsa‐miR‐196a‐5p, hsa‐miR‐375, hsa‐let‐7d‐5p, hsa‐miR‐146b‐3p, hsa‐miR‐221‐5p, hsa‐miR‐205‐5p, hsa‐miR‐491‐5p), which have been previously identified to play a role in SCC development, using real time‐PCR with the Qiagen miRCURY LNA SYBR Green PCR Kit. RESULTS: We observed a significant upregulation of microRNA‐155, microRNA‐196a, microRNA‐375, and microRNA‐221 in cases with lymphovascular invasion. Morphologically, we identified poor differentiation, dysplasia, loss of membrane polarity, high nuclear to cytoplasmic ratio, and the presence of squamous nests as defining features of LVI. Additionally, we found a gender bias and observed a tendency toward lymphatic invasion in lesions presenting around the perineal and abdominal regions. CONCLUSION: We speculate that this profile may have prognostic significance and could guide the clinician in their treatment protocols for patients matching our genetic, demographic, and morphologic profile.
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spelling pubmed-96950832022-11-28 The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study Robison, Shayene Ngwenya, Sharol Molaudzi, Mulalo Molepo, Julitha Adeola, Henry Magangane, Pumza Health Sci Rep Original Research BACKGROUND AND AIMS: Lymphovascular invasion (LVI) is an indicator of lymph node metastasis and poor prognosis in various cancers including squamous cell carcinoma (SCC). Despite being easily resectable and having little potential for LVI; SCC displays aggressive behavior and often results in the death of the patient. With this in mind, it may be useful to investigate the clinical, pathological, and microRNA expression profile associated with LVI in SCC. METHODS: We evaluated the histological hallmarks associated with LVI from 16 formalin fixed paraffin embedded (FFPE) tissue samples (10 LVI−, 6 LVI+). We also quantified the expression of 10 microRNAs (hsa‐miR‐21‐5p, hsa‐miR‐21‐3p, hsa‐miR‐155‐5p, hsa‐miR‐196a‐5p, hsa‐miR‐375, hsa‐let‐7d‐5p, hsa‐miR‐146b‐3p, hsa‐miR‐221‐5p, hsa‐miR‐205‐5p, hsa‐miR‐491‐5p), which have been previously identified to play a role in SCC development, using real time‐PCR with the Qiagen miRCURY LNA SYBR Green PCR Kit. RESULTS: We observed a significant upregulation of microRNA‐155, microRNA‐196a, microRNA‐375, and microRNA‐221 in cases with lymphovascular invasion. Morphologically, we identified poor differentiation, dysplasia, loss of membrane polarity, high nuclear to cytoplasmic ratio, and the presence of squamous nests as defining features of LVI. Additionally, we found a gender bias and observed a tendency toward lymphatic invasion in lesions presenting around the perineal and abdominal regions. CONCLUSION: We speculate that this profile may have prognostic significance and could guide the clinician in their treatment protocols for patients matching our genetic, demographic, and morphologic profile. John Wiley and Sons Inc. 2022-11-25 /pmc/articles/PMC9695083/ /pubmed/36447566 http://dx.doi.org/10.1002/hsr2.958 Text en © 2022 The Authors. Health Science Reports published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Robison, Shayene
Ngwenya, Sharol
Molaudzi, Mulalo
Molepo, Julitha
Adeola, Henry
Magangane, Pumza
The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study
title The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study
title_full The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study
title_fullStr The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study
title_full_unstemmed The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study
title_short The clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: A basic descriptive study
title_sort clinicopathological and microrna expression signature associated with lymphovascular invasion in squamous cell carcinoma: a basic descriptive study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695083/
https://www.ncbi.nlm.nih.gov/pubmed/36447566
http://dx.doi.org/10.1002/hsr2.958
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