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Evaluation of Auranofin Loading within Ferritin Nanocages
Auranofin (AF), a gold(I) compound that is currently used for the treatment of rheumatoid arthritis and is in clinical trials for its promising anticancer activity, was encapsulated within the human H-chain and the horse spleen ferritin nanocages using the alkaline disassembly/reassembly protocol. T...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695178/ https://www.ncbi.nlm.nih.gov/pubmed/36430642 http://dx.doi.org/10.3390/ijms232214162 |
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author | Lucignano, Rosanna Pratesi, Alessandro Imbimbo, Paola Monti, Daria Maria Picone, Delia Messori, Luigi Ferraro, Giarita Merlino, Antonello |
author_facet | Lucignano, Rosanna Pratesi, Alessandro Imbimbo, Paola Monti, Daria Maria Picone, Delia Messori, Luigi Ferraro, Giarita Merlino, Antonello |
author_sort | Lucignano, Rosanna |
collection | PubMed |
description | Auranofin (AF), a gold(I) compound that is currently used for the treatment of rheumatoid arthritis and is in clinical trials for its promising anticancer activity, was encapsulated within the human H-chain and the horse spleen ferritin nanocages using the alkaline disassembly/reassembly protocol. The aim of the work was to highlight possible differences in their drug loading capacity and efficacy. The drug-loaded ferritins were characterized via UV-vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy to assess AF encapsulation and to define the exact amount of gold atoms trapped in the Ft cavity. The crystal structures allowed us to define the nature of AF interaction with both ferritins and to identify the gold binding sites. Moreover, the biological characterization let us to obtain preliminary information on the cytotoxic effect of AF when bound to the human H-chain. |
format | Online Article Text |
id | pubmed-9695178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96951782022-11-26 Evaluation of Auranofin Loading within Ferritin Nanocages Lucignano, Rosanna Pratesi, Alessandro Imbimbo, Paola Monti, Daria Maria Picone, Delia Messori, Luigi Ferraro, Giarita Merlino, Antonello Int J Mol Sci Article Auranofin (AF), a gold(I) compound that is currently used for the treatment of rheumatoid arthritis and is in clinical trials for its promising anticancer activity, was encapsulated within the human H-chain and the horse spleen ferritin nanocages using the alkaline disassembly/reassembly protocol. The aim of the work was to highlight possible differences in their drug loading capacity and efficacy. The drug-loaded ferritins were characterized via UV-vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy to assess AF encapsulation and to define the exact amount of gold atoms trapped in the Ft cavity. The crystal structures allowed us to define the nature of AF interaction with both ferritins and to identify the gold binding sites. Moreover, the biological characterization let us to obtain preliminary information on the cytotoxic effect of AF when bound to the human H-chain. MDPI 2022-11-16 /pmc/articles/PMC9695178/ /pubmed/36430642 http://dx.doi.org/10.3390/ijms232214162 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lucignano, Rosanna Pratesi, Alessandro Imbimbo, Paola Monti, Daria Maria Picone, Delia Messori, Luigi Ferraro, Giarita Merlino, Antonello Evaluation of Auranofin Loading within Ferritin Nanocages |
title | Evaluation of Auranofin Loading within Ferritin Nanocages |
title_full | Evaluation of Auranofin Loading within Ferritin Nanocages |
title_fullStr | Evaluation of Auranofin Loading within Ferritin Nanocages |
title_full_unstemmed | Evaluation of Auranofin Loading within Ferritin Nanocages |
title_short | Evaluation of Auranofin Loading within Ferritin Nanocages |
title_sort | evaluation of auranofin loading within ferritin nanocages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695178/ https://www.ncbi.nlm.nih.gov/pubmed/36430642 http://dx.doi.org/10.3390/ijms232214162 |
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