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Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines

The current work demonstrates a comparative study between aerial and root parts of Zygophyllum album L. The total phenolic (TPC) and flavonoid content (TFC), in addition to the antioxidant activity, of the crude extracts were investigated, where the aerial parts revealed a higher value overall. By m...

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Autores principales: Abdelhameed, Reda F. A., Nafie, Mohamed S., Hal, Dina M., Nasr, Ali M., Swidan, Shady A., Abdel-Kader, Maged S., Ibrahim, Amany K., Ahmed, Safwat A., Badr, Jihan M., Eltamany, Enas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695243/
https://www.ncbi.nlm.nih.gov/pubmed/36355507
http://dx.doi.org/10.3390/ph15111334
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author Abdelhameed, Reda F. A.
Nafie, Mohamed S.
Hal, Dina M.
Nasr, Ali M.
Swidan, Shady A.
Abdel-Kader, Maged S.
Ibrahim, Amany K.
Ahmed, Safwat A.
Badr, Jihan M.
Eltamany, Enas E.
author_facet Abdelhameed, Reda F. A.
Nafie, Mohamed S.
Hal, Dina M.
Nasr, Ali M.
Swidan, Shady A.
Abdel-Kader, Maged S.
Ibrahim, Amany K.
Ahmed, Safwat A.
Badr, Jihan M.
Eltamany, Enas E.
author_sort Abdelhameed, Reda F. A.
collection PubMed
description The current work demonstrates a comparative study between aerial and root parts of Zygophyllum album L. The total phenolic (TPC) and flavonoid content (TFC), in addition to the antioxidant activity, of the crude extracts were investigated, where the aerial parts revealed a higher value overall. By means of UV–VIS and HPLC, rutin and caffeic acid were detected and then quantified as 5.91 and 0.97 mg/g of the plant extract, respectively. Moreover, the biosynthesis of AgNPs utilizing the crude extract of the arial parts and root of Z. album L. and the phenolic extracts was achieved in an attempt to enhance the cytotoxicity of the different plant extracts. The prepared AgNPs formulations were characterized by TEM and zeta potential measurements, which revealed that all of the formulated AgNPs were of a small particle diameter and were highly stable. The mean hydrodynamic particle size ranged from 67.11 to 80.04 nm, while the zeta potential ranged from 29.1 to 38.6 mV. Upon biosynthesis of the AgNPs using the extracts, the cytotoxicity of the tested samples was improved, so the polyphenolics AgNPs of the aerial parts exhibited a potent cytotoxicity against lung A549 and prostate PC-3 cancer cells with IC(50) values of 6.1 and 4.36 µg/mL, respectively, compared with Doxorubicin (IC(50) values of 6.19 and 5.13 µg/mL, respectively). Regarding the apoptotic activity, polyphenolics AgNPs of the aerial parts induced apoptotic cell death by 4.2-fold in PC-3 and 4.7-fold in A549 cells compared with the untreated control. The mechanism of apoptosis in both cancerous cells appeared to be via the upregulation proapoptotic genes; p53, Bax, caspase 3, 8, and 9, and the downregulation of antiapoptotic gene, Bcl-2. Hence, this formula may serve as a good source for anticancer agents against PC-3 and A549 cells.
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spelling pubmed-96952432022-11-26 Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines Abdelhameed, Reda F. A. Nafie, Mohamed S. Hal, Dina M. Nasr, Ali M. Swidan, Shady A. Abdel-Kader, Maged S. Ibrahim, Amany K. Ahmed, Safwat A. Badr, Jihan M. Eltamany, Enas E. Pharmaceuticals (Basel) Article The current work demonstrates a comparative study between aerial and root parts of Zygophyllum album L. The total phenolic (TPC) and flavonoid content (TFC), in addition to the antioxidant activity, of the crude extracts were investigated, where the aerial parts revealed a higher value overall. By means of UV–VIS and HPLC, rutin and caffeic acid were detected and then quantified as 5.91 and 0.97 mg/g of the plant extract, respectively. Moreover, the biosynthesis of AgNPs utilizing the crude extract of the arial parts and root of Z. album L. and the phenolic extracts was achieved in an attempt to enhance the cytotoxicity of the different plant extracts. The prepared AgNPs formulations were characterized by TEM and zeta potential measurements, which revealed that all of the formulated AgNPs were of a small particle diameter and were highly stable. The mean hydrodynamic particle size ranged from 67.11 to 80.04 nm, while the zeta potential ranged from 29.1 to 38.6 mV. Upon biosynthesis of the AgNPs using the extracts, the cytotoxicity of the tested samples was improved, so the polyphenolics AgNPs of the aerial parts exhibited a potent cytotoxicity against lung A549 and prostate PC-3 cancer cells with IC(50) values of 6.1 and 4.36 µg/mL, respectively, compared with Doxorubicin (IC(50) values of 6.19 and 5.13 µg/mL, respectively). Regarding the apoptotic activity, polyphenolics AgNPs of the aerial parts induced apoptotic cell death by 4.2-fold in PC-3 and 4.7-fold in A549 cells compared with the untreated control. The mechanism of apoptosis in both cancerous cells appeared to be via the upregulation proapoptotic genes; p53, Bax, caspase 3, 8, and 9, and the downregulation of antiapoptotic gene, Bcl-2. Hence, this formula may serve as a good source for anticancer agents against PC-3 and A549 cells. MDPI 2022-10-28 /pmc/articles/PMC9695243/ /pubmed/36355507 http://dx.doi.org/10.3390/ph15111334 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abdelhameed, Reda F. A.
Nafie, Mohamed S.
Hal, Dina M.
Nasr, Ali M.
Swidan, Shady A.
Abdel-Kader, Maged S.
Ibrahim, Amany K.
Ahmed, Safwat A.
Badr, Jihan M.
Eltamany, Enas E.
Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines
title Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines
title_full Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines
title_fullStr Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines
title_full_unstemmed Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines
title_short Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines
title_sort comparative cytotoxic evaluation of zygophyllum album root and aerial parts of different extracts and their biosynthesized silver nanoparticles on lung a549 and prostate pc-3 cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695243/
https://www.ncbi.nlm.nih.gov/pubmed/36355507
http://dx.doi.org/10.3390/ph15111334
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