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Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum

Vibrio species are widely distributed and can be potentially pathogenic to aquatic organisms. In this study, we isolated Vibrio spp. from environmental samples (seawater, sediment, and fish swabs) collected over a three-year period from a fish farm in Mali Ston Bay in the Adriatic Sea, Croatia, and...

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Autores principales: Kapetanović, Damir, Vardić Smrzlić, Irena, Gavrilović, Ana, Jug-Dujaković, Jurica, Perić, Lorena, Kazazić, Snježana, Mišić Radić, Tea, Kolda, Anamarija, Čanković, Milan, Žunić, Jakov, Listeš, Eddy, Vukić Lušić, Darija, Lillehaug, Atle, Lončarević, Semir, Pikelj, Kristina, Hengl, Brigita, Knežević, Dražen, El-Matbouli, Mansour
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695460/
https://www.ncbi.nlm.nih.gov/pubmed/36363751
http://dx.doi.org/10.3390/microorganisms10112159
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author Kapetanović, Damir
Vardić Smrzlić, Irena
Gavrilović, Ana
Jug-Dujaković, Jurica
Perić, Lorena
Kazazić, Snježana
Mišić Radić, Tea
Kolda, Anamarija
Čanković, Milan
Žunić, Jakov
Listeš, Eddy
Vukić Lušić, Darija
Lillehaug, Atle
Lončarević, Semir
Pikelj, Kristina
Hengl, Brigita
Knežević, Dražen
El-Matbouli, Mansour
author_facet Kapetanović, Damir
Vardić Smrzlić, Irena
Gavrilović, Ana
Jug-Dujaković, Jurica
Perić, Lorena
Kazazić, Snježana
Mišić Radić, Tea
Kolda, Anamarija
Čanković, Milan
Žunić, Jakov
Listeš, Eddy
Vukić Lušić, Darija
Lillehaug, Atle
Lončarević, Semir
Pikelj, Kristina
Hengl, Brigita
Knežević, Dražen
El-Matbouli, Mansour
author_sort Kapetanović, Damir
collection PubMed
description Vibrio species are widely distributed and can be potentially pathogenic to aquatic organisms. In this study, we isolated Vibrio spp. from environmental samples (seawater, sediment, and fish swabs) collected over a three-year period from a fish farm in Mali Ston Bay in the Adriatic Sea, Croatia, and assess their distribution. A total of 48 seawater samples and 12 sediment samples, as well as gill and skin swabs from 110 farmed European seabass, were analysed for the presence of Vibrio. Vibrio strains were identified to the species level by MALDI TOF MS. The analysis revealed that V. alginolyticus was the predominant species in European seabass, followed by V. anguillarum. V. alginolyticus was isolated from the sediments, along with V. gigantis and V. pomeroyi, while V. chagasii, V. cyclitrophicus, V. fortis, V. gigantis, V. harveyi, V. pelagius, and V. pomeroyi were isolated from seawater. V. anguillarum was isolated only twice during two different spring seasons, once from a diseased sea bass and the second time from a healthy sea bass. We analysed these two isolates and found that they differ both genetically and in terms of resistance to antibiotics. Our results confirm the seasonality of vibriosis incidence and the presence of the pathogenic V. anguillarum, which increases the risk of vibriosis.
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spelling pubmed-96954602022-11-26 Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum Kapetanović, Damir Vardić Smrzlić, Irena Gavrilović, Ana Jug-Dujaković, Jurica Perić, Lorena Kazazić, Snježana Mišić Radić, Tea Kolda, Anamarija Čanković, Milan Žunić, Jakov Listeš, Eddy Vukić Lušić, Darija Lillehaug, Atle Lončarević, Semir Pikelj, Kristina Hengl, Brigita Knežević, Dražen El-Matbouli, Mansour Microorganisms Article Vibrio species are widely distributed and can be potentially pathogenic to aquatic organisms. In this study, we isolated Vibrio spp. from environmental samples (seawater, sediment, and fish swabs) collected over a three-year period from a fish farm in Mali Ston Bay in the Adriatic Sea, Croatia, and assess their distribution. A total of 48 seawater samples and 12 sediment samples, as well as gill and skin swabs from 110 farmed European seabass, were analysed for the presence of Vibrio. Vibrio strains were identified to the species level by MALDI TOF MS. The analysis revealed that V. alginolyticus was the predominant species in European seabass, followed by V. anguillarum. V. alginolyticus was isolated from the sediments, along with V. gigantis and V. pomeroyi, while V. chagasii, V. cyclitrophicus, V. fortis, V. gigantis, V. harveyi, V. pelagius, and V. pomeroyi were isolated from seawater. V. anguillarum was isolated only twice during two different spring seasons, once from a diseased sea bass and the second time from a healthy sea bass. We analysed these two isolates and found that they differ both genetically and in terms of resistance to antibiotics. Our results confirm the seasonality of vibriosis incidence and the presence of the pathogenic V. anguillarum, which increases the risk of vibriosis. MDPI 2022-10-31 /pmc/articles/PMC9695460/ /pubmed/36363751 http://dx.doi.org/10.3390/microorganisms10112159 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kapetanović, Damir
Vardić Smrzlić, Irena
Gavrilović, Ana
Jug-Dujaković, Jurica
Perić, Lorena
Kazazić, Snježana
Mišić Radić, Tea
Kolda, Anamarija
Čanković, Milan
Žunić, Jakov
Listeš, Eddy
Vukić Lušić, Darija
Lillehaug, Atle
Lončarević, Semir
Pikelj, Kristina
Hengl, Brigita
Knežević, Dražen
El-Matbouli, Mansour
Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum
title Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum
title_full Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum
title_fullStr Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum
title_full_unstemmed Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum
title_short Characterization of Vibrio Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on V. anguillarum
title_sort characterization of vibrio populations from cultured european seabass and the surrounding marine environment with emphasis on v. anguillarum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695460/
https://www.ncbi.nlm.nih.gov/pubmed/36363751
http://dx.doi.org/10.3390/microorganisms10112159
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