Cargando…

Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics

Clostridium autoethanogenum protein (CAP) is a new single-cell protein explored in aquatic feeds in recent years. This study investigated the dietary effects of CAP replacing fishmeal (FM) on the growth, intestinal histology and flesh metabolism of largemouth bass (Micropterus salmoides). In a basal...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Pinxian, Li, Xiaoqin, Yao, Wenxiang, Li, Menglu, Wang, Yuanyuan, Leng, Xiangjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695463/
https://www.ncbi.nlm.nih.gov/pubmed/36355171
http://dx.doi.org/10.3390/metabo12111088
_version_ 1784838066417958912
author Yang, Pinxian
Li, Xiaoqin
Yao, Wenxiang
Li, Menglu
Wang, Yuanyuan
Leng, Xiangjun
author_facet Yang, Pinxian
Li, Xiaoqin
Yao, Wenxiang
Li, Menglu
Wang, Yuanyuan
Leng, Xiangjun
author_sort Yang, Pinxian
collection PubMed
description Clostridium autoethanogenum protein (CAP) is a new single-cell protein explored in aquatic feeds in recent years. This study investigated the dietary effects of CAP replacing fishmeal (FM) on the growth, intestinal histology and flesh metabolism of largemouth bass (Micropterus salmoides). In a basal diet containing 700 g/kg of FM, CAP was used to substitute 0%, 15%, 30%, 45%, 70% and 100% of dietary FM to form six isonitrogenous diets (Con, CAP-15, CAP-30, CAP-45, CAP-70, CAP-100) to feed largemouth bass (80.0 g) for 12 weeks. Only the CAP-100 group showed significantly lower weight gain (WG) and a higher feed conversion ratio (FCR) than the control (p < 0.05). A broken-line analysis based on WG and FCR showed that the suitable replacement of FM with CAP was 67.1–68.0%. The flesh n-3/n-6 polyunsaturated fatty acid, intestinal protease activity, villi width and height in the CAP-100 group were significantly lower than those in the control group (p < 0.05). The Kyoto Encyclopedia of Genes and Genomes analysis showed that the metabolic pathway in flesh was mainly enriched in the “lipid metabolic pathway”, “amino acid metabolism”, “endocrine system” and “carbohydrate metabolism”. In conclusion, CAP could successfully replace 67.1–68.0% of dietary FM, while the complete substitution decreased the growth, damaged the intestinal morphology and down-regulated the lipid metabolites.
format Online
Article
Text
id pubmed-9695463
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96954632022-11-26 Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics Yang, Pinxian Li, Xiaoqin Yao, Wenxiang Li, Menglu Wang, Yuanyuan Leng, Xiangjun Metabolites Article Clostridium autoethanogenum protein (CAP) is a new single-cell protein explored in aquatic feeds in recent years. This study investigated the dietary effects of CAP replacing fishmeal (FM) on the growth, intestinal histology and flesh metabolism of largemouth bass (Micropterus salmoides). In a basal diet containing 700 g/kg of FM, CAP was used to substitute 0%, 15%, 30%, 45%, 70% and 100% of dietary FM to form six isonitrogenous diets (Con, CAP-15, CAP-30, CAP-45, CAP-70, CAP-100) to feed largemouth bass (80.0 g) for 12 weeks. Only the CAP-100 group showed significantly lower weight gain (WG) and a higher feed conversion ratio (FCR) than the control (p < 0.05). A broken-line analysis based on WG and FCR showed that the suitable replacement of FM with CAP was 67.1–68.0%. The flesh n-3/n-6 polyunsaturated fatty acid, intestinal protease activity, villi width and height in the CAP-100 group were significantly lower than those in the control group (p < 0.05). The Kyoto Encyclopedia of Genes and Genomes analysis showed that the metabolic pathway in flesh was mainly enriched in the “lipid metabolic pathway”, “amino acid metabolism”, “endocrine system” and “carbohydrate metabolism”. In conclusion, CAP could successfully replace 67.1–68.0% of dietary FM, while the complete substitution decreased the growth, damaged the intestinal morphology and down-regulated the lipid metabolites. MDPI 2022-11-09 /pmc/articles/PMC9695463/ /pubmed/36355171 http://dx.doi.org/10.3390/metabo12111088 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Pinxian
Li, Xiaoqin
Yao, Wenxiang
Li, Menglu
Wang, Yuanyuan
Leng, Xiangjun
Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics
title Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics
title_full Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics
title_fullStr Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics
title_full_unstemmed Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics
title_short Dietary Effect of Clostridium autoethanogenum Protein on Growth, Intestinal Histology and Flesh Lipid Metabolism of Largemouth Bass (Micropterus salmoides) Based on Metabolomics
title_sort dietary effect of clostridium autoethanogenum protein on growth, intestinal histology and flesh lipid metabolism of largemouth bass (micropterus salmoides) based on metabolomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695463/
https://www.ncbi.nlm.nih.gov/pubmed/36355171
http://dx.doi.org/10.3390/metabo12111088
work_keys_str_mv AT yangpinxian dietaryeffectofclostridiumautoethanogenumproteinongrowthintestinalhistologyandfleshlipidmetabolismoflargemouthbassmicropterussalmoidesbasedonmetabolomics
AT lixiaoqin dietaryeffectofclostridiumautoethanogenumproteinongrowthintestinalhistologyandfleshlipidmetabolismoflargemouthbassmicropterussalmoidesbasedonmetabolomics
AT yaowenxiang dietaryeffectofclostridiumautoethanogenumproteinongrowthintestinalhistologyandfleshlipidmetabolismoflargemouthbassmicropterussalmoidesbasedonmetabolomics
AT limenglu dietaryeffectofclostridiumautoethanogenumproteinongrowthintestinalhistologyandfleshlipidmetabolismoflargemouthbassmicropterussalmoidesbasedonmetabolomics
AT wangyuanyuan dietaryeffectofclostridiumautoethanogenumproteinongrowthintestinalhistologyandfleshlipidmetabolismoflargemouthbassmicropterussalmoidesbasedonmetabolomics
AT lengxiangjun dietaryeffectofclostridiumautoethanogenumproteinongrowthintestinalhistologyandfleshlipidmetabolismoflargemouthbassmicropterussalmoidesbasedonmetabolomics