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Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal

Vaccinium myrtillus is a dwarf shrub of the Ericaceae family with a Palearctic distribution, associated with temperate and cold humid climates. It is widespread on the European continent; on the Iberian Peninsula it is located on Atlantic climate mountains and glacial relicts. In Portugal, we find s...

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Autores principales: Gonçalves, Ana C., Sánchez-Juanes, Fernando, Meirinho, Sara, Silva, Luís R., Alves, Gilberto, Flores-Félix, José David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695653/
https://www.ncbi.nlm.nih.gov/pubmed/36363783
http://dx.doi.org/10.3390/microorganisms10112193
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author Gonçalves, Ana C.
Sánchez-Juanes, Fernando
Meirinho, Sara
Silva, Luís R.
Alves, Gilberto
Flores-Félix, José David
author_facet Gonçalves, Ana C.
Sánchez-Juanes, Fernando
Meirinho, Sara
Silva, Luís R.
Alves, Gilberto
Flores-Félix, José David
author_sort Gonçalves, Ana C.
collection PubMed
description Vaccinium myrtillus is a dwarf shrub of the Ericaceae family with a Palearctic distribution, associated with temperate and cold humid climates. It is widespread on the European continent; on the Iberian Peninsula it is located on Atlantic climate mountains and glacial relicts. In Portugal, we find scattered and interesting populations; however, the majority of them are threatened by climate change and wildfires. Given that, the objective of this study is to determine the rhizospheric and root bacterial communities of this plant in the southernmost regions, and, consequently, its potential range and ability to be used as a biofertilizer. In this work, metabarcoding of 16S rRNA gene showed that the endophytic bacterial diversity is dependent on the plant and selected by it according to the observed alpha and beta diversity. Moreover, a culturomic approach allowed 142 different strains to be isolated, some of them being putative new species. Additionally, some strains belonging to the genera Bacillus, Paenibacillus, Pseudomonas, Paraburkholderia, and Caballeronia showed significant potential to be applied as multifunctional biofertilizers since they present good plant growth-promoting (PGP) mechanisms, high colonization capacities, and an increase in vegetative parameters in blueberry and tomato plants.
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spelling pubmed-96956532022-11-26 Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal Gonçalves, Ana C. Sánchez-Juanes, Fernando Meirinho, Sara Silva, Luís R. Alves, Gilberto Flores-Félix, José David Microorganisms Article Vaccinium myrtillus is a dwarf shrub of the Ericaceae family with a Palearctic distribution, associated with temperate and cold humid climates. It is widespread on the European continent; on the Iberian Peninsula it is located on Atlantic climate mountains and glacial relicts. In Portugal, we find scattered and interesting populations; however, the majority of them are threatened by climate change and wildfires. Given that, the objective of this study is to determine the rhizospheric and root bacterial communities of this plant in the southernmost regions, and, consequently, its potential range and ability to be used as a biofertilizer. In this work, metabarcoding of 16S rRNA gene showed that the endophytic bacterial diversity is dependent on the plant and selected by it according to the observed alpha and beta diversity. Moreover, a culturomic approach allowed 142 different strains to be isolated, some of them being putative new species. Additionally, some strains belonging to the genera Bacillus, Paenibacillus, Pseudomonas, Paraburkholderia, and Caballeronia showed significant potential to be applied as multifunctional biofertilizers since they present good plant growth-promoting (PGP) mechanisms, high colonization capacities, and an increase in vegetative parameters in blueberry and tomato plants. MDPI 2022-11-04 /pmc/articles/PMC9695653/ /pubmed/36363783 http://dx.doi.org/10.3390/microorganisms10112193 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gonçalves, Ana C.
Sánchez-Juanes, Fernando
Meirinho, Sara
Silva, Luís R.
Alves, Gilberto
Flores-Félix, José David
Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal
title Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal
title_full Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal
title_fullStr Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal
title_full_unstemmed Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal
title_short Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal
title_sort insight into the taxonomic and functional diversity of bacterial communities inhabiting blueberries in portugal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695653/
https://www.ncbi.nlm.nih.gov/pubmed/36363783
http://dx.doi.org/10.3390/microorganisms10112193
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