Cargando…

Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients

We sought to investigate the influence of SARS-CoV-2 infection on the cytokine profiles of peripheral blood mononuclear cells (PBMCs) and neutrophils from coronavirus disease 2019 (COVID-19) intensive care unit (ICU) patients. Neutrophils and PBMCs were separated and stimulated with the mitogen phyt...

Descripción completa

Detalles Bibliográficos
Autores principales: Essa, Sahar, Shamsah, Mohammed, Alsarraf, Abdalaziz H., Esmaeil, Ali, Al-Shammasi, Ahmed, Raghupathy, Raj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695671/
https://www.ncbi.nlm.nih.gov/pubmed/36363785
http://dx.doi.org/10.3390/microorganisms10112194
_version_ 1784838120443740160
author Essa, Sahar
Shamsah, Mohammed
Alsarraf, Abdalaziz H.
Esmaeil, Ali
Al-Shammasi, Ahmed
Raghupathy, Raj
author_facet Essa, Sahar
Shamsah, Mohammed
Alsarraf, Abdalaziz H.
Esmaeil, Ali
Al-Shammasi, Ahmed
Raghupathy, Raj
author_sort Essa, Sahar
collection PubMed
description We sought to investigate the influence of SARS-CoV-2 infection on the cytokine profiles of peripheral blood mononuclear cells (PBMCs) and neutrophils from coronavirus disease 2019 (COVID-19) intensive care unit (ICU) patients. Neutrophils and PBMCs were separated and stimulated with the mitogen phytohemagglutinin. Culture supernatants of mitogen-stimulated PBMCs and neutrophils from 88 COVID-19 ICU patients and 88 healthy controls were evaluated for levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-α, IFN-γ, interleukin (IL)-2, -4, -5, -6, -9, -10, -12, -17A, and tumor necrosis factor (TNF)-α using anti-cytokine antibody MACSPlex capture beads. Cytokine profiles of PBMCs showed significantly lower levels of GM-CSF, IFN-γ, IL-6, IL-9, IL-10, IL-17A, and TNF-α (p < 0.0001) in COVID-19 ICU patients. In contrast, COVID-19 ICU patients showed higher median levels of IL-2 (p < 0.001) and IL-5 (p < 0.01) by PBMCs. As for neutrophils, COVID-19 ICU patients showed significantly lower levels of GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-17A, IL-12, TNF-α (p < 0.0001), and IFN-α (p < 0.01). T-helper (Th)1:Th2 cytokine ratios revealed lower inflammatory cytokine for PBMCs and neutrophils in COVID-19 ICU patients. Cytokine production profiles and Th1:Th2 cytokine ratios suggest that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has an immunomodulatory effect on PBMCs and neutrophils. This study also suggests that the increased levels of several cytokines in the serum are not sourced from PBMCs and neutrophils.
format Online
Article
Text
id pubmed-9695671
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96956712022-11-26 Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients Essa, Sahar Shamsah, Mohammed Alsarraf, Abdalaziz H. Esmaeil, Ali Al-Shammasi, Ahmed Raghupathy, Raj Microorganisms Article We sought to investigate the influence of SARS-CoV-2 infection on the cytokine profiles of peripheral blood mononuclear cells (PBMCs) and neutrophils from coronavirus disease 2019 (COVID-19) intensive care unit (ICU) patients. Neutrophils and PBMCs were separated and stimulated with the mitogen phytohemagglutinin. Culture supernatants of mitogen-stimulated PBMCs and neutrophils from 88 COVID-19 ICU patients and 88 healthy controls were evaluated for levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-α, IFN-γ, interleukin (IL)-2, -4, -5, -6, -9, -10, -12, -17A, and tumor necrosis factor (TNF)-α using anti-cytokine antibody MACSPlex capture beads. Cytokine profiles of PBMCs showed significantly lower levels of GM-CSF, IFN-γ, IL-6, IL-9, IL-10, IL-17A, and TNF-α (p < 0.0001) in COVID-19 ICU patients. In contrast, COVID-19 ICU patients showed higher median levels of IL-2 (p < 0.001) and IL-5 (p < 0.01) by PBMCs. As for neutrophils, COVID-19 ICU patients showed significantly lower levels of GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-17A, IL-12, TNF-α (p < 0.0001), and IFN-α (p < 0.01). T-helper (Th)1:Th2 cytokine ratios revealed lower inflammatory cytokine for PBMCs and neutrophils in COVID-19 ICU patients. Cytokine production profiles and Th1:Th2 cytokine ratios suggest that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has an immunomodulatory effect on PBMCs and neutrophils. This study also suggests that the increased levels of several cytokines in the serum are not sourced from PBMCs and neutrophils. MDPI 2022-11-04 /pmc/articles/PMC9695671/ /pubmed/36363785 http://dx.doi.org/10.3390/microorganisms10112194 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Essa, Sahar
Shamsah, Mohammed
Alsarraf, Abdalaziz H.
Esmaeil, Ali
Al-Shammasi, Ahmed
Raghupathy, Raj
Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients
title Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients
title_full Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients
title_fullStr Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients
title_full_unstemmed Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients
title_short Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients
title_sort influence of sars-cov-2 infection on cytokine production by mitogen-stimulated peripheral blood mononuclear cells and neutrophils in covid-19 intensive care unit patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695671/
https://www.ncbi.nlm.nih.gov/pubmed/36363785
http://dx.doi.org/10.3390/microorganisms10112194
work_keys_str_mv AT essasahar influenceofsarscov2infectiononcytokineproductionbymitogenstimulatedperipheralbloodmononuclearcellsandneutrophilsincovid19intensivecareunitpatients
AT shamsahmohammed influenceofsarscov2infectiononcytokineproductionbymitogenstimulatedperipheralbloodmononuclearcellsandneutrophilsincovid19intensivecareunitpatients
AT alsarrafabdalazizh influenceofsarscov2infectiononcytokineproductionbymitogenstimulatedperipheralbloodmononuclearcellsandneutrophilsincovid19intensivecareunitpatients
AT esmaeilali influenceofsarscov2infectiononcytokineproductionbymitogenstimulatedperipheralbloodmononuclearcellsandneutrophilsincovid19intensivecareunitpatients
AT alshammasiahmed influenceofsarscov2infectiononcytokineproductionbymitogenstimulatedperipheralbloodmononuclearcellsandneutrophilsincovid19intensivecareunitpatients
AT raghupathyraj influenceofsarscov2infectiononcytokineproductionbymitogenstimulatedperipheralbloodmononuclearcellsandneutrophilsincovid19intensivecareunitpatients