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Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary

Grafting cultivars onto rootstocks is a widely used practice by the apple industry predominantly aimed at faster fruit bearing. Using high-throughput sequencing, we revealed the presence of recently described viral agents, namely apple hammerhead viroid (AHVd), apple luteovirus 1 (ALV-1), and citrus...

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Autores principales: Várallyay, Eva, Přibylová, Jaroslava, Galbacs, Zsuzsanna Nagyne, Jahan, Almash, Varga, Tunde, Špak, Josef, Lenz, Ondřej, Fránová, Jana, Sedlák, Jiří, Koloniuk, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695845/
https://www.ncbi.nlm.nih.gov/pubmed/36366445
http://dx.doi.org/10.3390/v14112347
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author Várallyay, Eva
Přibylová, Jaroslava
Galbacs, Zsuzsanna Nagyne
Jahan, Almash
Varga, Tunde
Špak, Josef
Lenz, Ondřej
Fránová, Jana
Sedlák, Jiří
Koloniuk, Igor
author_facet Várallyay, Eva
Přibylová, Jaroslava
Galbacs, Zsuzsanna Nagyne
Jahan, Almash
Varga, Tunde
Špak, Josef
Lenz, Ondřej
Fránová, Jana
Sedlák, Jiří
Koloniuk, Igor
author_sort Várallyay, Eva
collection PubMed
description Grafting cultivars onto rootstocks is a widely used practice by the apple industry predominantly aimed at faster fruit bearing. Using high-throughput sequencing, we revealed the presence of recently described viral agents, namely apple hammerhead viroid (AHVd), apple luteovirus 1 (ALV-1), and citrus concave gum-associated virus (CCGaV), in germplasm collections and production orchards in the Czech Republic and Hungary. The HTS results were validated with RT-(q)PCR, and Northern blotting techniques. To obtain further insight about the presence of these agents, RT-PCR based surveys were carried out and showed their widespread presence alone or in mixed infections. The pathogens were present both in production areas and in feral samples. In addition, rootstock-to-scion transmission of ALV-1 and CCGaV was confirmed using commercial rootstock materials. Phylogenetic relationships based on partial sequences of distinct variants were also investigated. Furthermore, the rosy apple aphid was found to be ALV-1-positive, suggesting that it might be a potential vector of the virus.
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spelling pubmed-96958452022-11-26 Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary Várallyay, Eva Přibylová, Jaroslava Galbacs, Zsuzsanna Nagyne Jahan, Almash Varga, Tunde Špak, Josef Lenz, Ondřej Fránová, Jana Sedlák, Jiří Koloniuk, Igor Viruses Article Grafting cultivars onto rootstocks is a widely used practice by the apple industry predominantly aimed at faster fruit bearing. Using high-throughput sequencing, we revealed the presence of recently described viral agents, namely apple hammerhead viroid (AHVd), apple luteovirus 1 (ALV-1), and citrus concave gum-associated virus (CCGaV), in germplasm collections and production orchards in the Czech Republic and Hungary. The HTS results were validated with RT-(q)PCR, and Northern blotting techniques. To obtain further insight about the presence of these agents, RT-PCR based surveys were carried out and showed their widespread presence alone or in mixed infections. The pathogens were present both in production areas and in feral samples. In addition, rootstock-to-scion transmission of ALV-1 and CCGaV was confirmed using commercial rootstock materials. Phylogenetic relationships based on partial sequences of distinct variants were also investigated. Furthermore, the rosy apple aphid was found to be ALV-1-positive, suggesting that it might be a potential vector of the virus. MDPI 2022-10-25 /pmc/articles/PMC9695845/ /pubmed/36366445 http://dx.doi.org/10.3390/v14112347 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Várallyay, Eva
Přibylová, Jaroslava
Galbacs, Zsuzsanna Nagyne
Jahan, Almash
Varga, Tunde
Špak, Josef
Lenz, Ondřej
Fránová, Jana
Sedlák, Jiří
Koloniuk, Igor
Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary
title Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary
title_full Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary
title_fullStr Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary
title_full_unstemmed Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary
title_short Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary
title_sort detection of apple hammerhead viroid, apple luteovirus 1 and citrus concave gum-associated virus in apple propagation materials and orchards in the czech republic and hungary
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695845/
https://www.ncbi.nlm.nih.gov/pubmed/36366445
http://dx.doi.org/10.3390/v14112347
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