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Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil
Infections with arboviruses are reported worldwide. Saint Louis encephalitis (SLEV) and West Nile viruses (WNV) are closely related flaviviruses affecting humans and animals. SLEV has been sporadically detected in humans, and corresponding antibodies have been frequently detected in horses throughou...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695862/ https://www.ncbi.nlm.nih.gov/pubmed/36366557 http://dx.doi.org/10.3390/v14112459 |
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author | Chalhoub, Flávia Löwen Levy Horta, Marco Aurélio Pereira Alcantara, Luiz Carlos Junior Morales, Alejandra dos Santos, Lilha Maria Barbosa Guerra-Campos, Vinícius Rodrigues, Cintia D. S. Santos, Carolina C. Mares-Guia, Maria Angélica M. Pauvolid-Corrêa, Alex de Filippis, Ana Maria Bispo |
author_facet | Chalhoub, Flávia Löwen Levy Horta, Marco Aurélio Pereira Alcantara, Luiz Carlos Junior Morales, Alejandra dos Santos, Lilha Maria Barbosa Guerra-Campos, Vinícius Rodrigues, Cintia D. S. Santos, Carolina C. Mares-Guia, Maria Angélica M. Pauvolid-Corrêa, Alex de Filippis, Ana Maria Bispo |
author_sort | Chalhoub, Flávia Löwen Levy |
collection | PubMed |
description | Infections with arboviruses are reported worldwide. Saint Louis encephalitis (SLEV) and West Nile viruses (WNV) are closely related flaviviruses affecting humans and animals. SLEV has been sporadically detected in humans, and corresponding antibodies have been frequently detected in horses throughout Brazil. WNV was first reported in western Brazil over a decade ago, has been associated with neurological disorders in humans and equines and its prevalence is increasing nationwide. Herein, we investigated by molecular and serological methods the presence or evidence of SLEV and WNV in equines from Rio de Janeiro. A total of 435 serum samples were collected from healthy horses and tested for specific neutralizing antibodies by plaque reduction neutralization test (PRNT(90)). Additionally, serum and central nervous system samples from 72 horses, including horses with neurological disorders resulting in a fatal outcome or horses which had contact with them, were tested by real-time reverse transcription–polymerase chain reaction (RT-qPCR) for both viruses. Adopting the criterion of four-fold antibody titer difference, 89 (20.4%) horses presented neutralizing antibodies for SLEV and five (1.1%) for WNV. No evidence of SLEV and WNV infection was detected by RT-qPCR and, thus, such infection could not be confirmed in the additional samples. Our findings indicate that horses from Rio de Janeiro were exposed to both SLEV and WNV, contributing to the current knowledge on the distribution of these viruses flaviviruses in Brazil. |
format | Online Article Text |
id | pubmed-9695862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96958622022-11-26 Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil Chalhoub, Flávia Löwen Levy Horta, Marco Aurélio Pereira Alcantara, Luiz Carlos Junior Morales, Alejandra dos Santos, Lilha Maria Barbosa Guerra-Campos, Vinícius Rodrigues, Cintia D. S. Santos, Carolina C. Mares-Guia, Maria Angélica M. Pauvolid-Corrêa, Alex de Filippis, Ana Maria Bispo Viruses Article Infections with arboviruses are reported worldwide. Saint Louis encephalitis (SLEV) and West Nile viruses (WNV) are closely related flaviviruses affecting humans and animals. SLEV has been sporadically detected in humans, and corresponding antibodies have been frequently detected in horses throughout Brazil. WNV was first reported in western Brazil over a decade ago, has been associated with neurological disorders in humans and equines and its prevalence is increasing nationwide. Herein, we investigated by molecular and serological methods the presence or evidence of SLEV and WNV in equines from Rio de Janeiro. A total of 435 serum samples were collected from healthy horses and tested for specific neutralizing antibodies by plaque reduction neutralization test (PRNT(90)). Additionally, serum and central nervous system samples from 72 horses, including horses with neurological disorders resulting in a fatal outcome or horses which had contact with them, were tested by real-time reverse transcription–polymerase chain reaction (RT-qPCR) for both viruses. Adopting the criterion of four-fold antibody titer difference, 89 (20.4%) horses presented neutralizing antibodies for SLEV and five (1.1%) for WNV. No evidence of SLEV and WNV infection was detected by RT-qPCR and, thus, such infection could not be confirmed in the additional samples. Our findings indicate that horses from Rio de Janeiro were exposed to both SLEV and WNV, contributing to the current knowledge on the distribution of these viruses flaviviruses in Brazil. MDPI 2022-11-06 /pmc/articles/PMC9695862/ /pubmed/36366557 http://dx.doi.org/10.3390/v14112459 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chalhoub, Flávia Löwen Levy Horta, Marco Aurélio Pereira Alcantara, Luiz Carlos Junior Morales, Alejandra dos Santos, Lilha Maria Barbosa Guerra-Campos, Vinícius Rodrigues, Cintia D. S. Santos, Carolina C. Mares-Guia, Maria Angélica M. Pauvolid-Corrêa, Alex de Filippis, Ana Maria Bispo Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil |
title | Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil |
title_full | Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil |
title_fullStr | Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil |
title_full_unstemmed | Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil |
title_short | Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil |
title_sort | serological evidence of exposure to saint louis encephalitis and west nile viruses in horses of rio de janeiro, brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695862/ https://www.ncbi.nlm.nih.gov/pubmed/36366557 http://dx.doi.org/10.3390/v14112459 |
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