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Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species

Onychomycosis is a fungal infection caused by different etiologic agents, including dermatophytes that specifically colonize keratin-rich substrates. The aim of this work was to investigate mechanical modifications of bovine membranes (used as an in vitro nail model) placed in contact with Trichophy...

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Autores principales: Temporiti, Marta Elisabetta Eleonora, Guerini, Marta, Baiguera, Rebecca Michela, Buratti, Simone, Desiderio, Anthea, Goppa, Lorenzo, Perugini, Paola, Savino, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695884/
https://www.ncbi.nlm.nih.gov/pubmed/36354900
http://dx.doi.org/10.3390/jof8111133
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author Temporiti, Marta Elisabetta Eleonora
Guerini, Marta
Baiguera, Rebecca Michela
Buratti, Simone
Desiderio, Anthea
Goppa, Lorenzo
Perugini, Paola
Savino, Elena
author_facet Temporiti, Marta Elisabetta Eleonora
Guerini, Marta
Baiguera, Rebecca Michela
Buratti, Simone
Desiderio, Anthea
Goppa, Lorenzo
Perugini, Paola
Savino, Elena
author_sort Temporiti, Marta Elisabetta Eleonora
collection PubMed
description Onychomycosis is a fungal infection caused by different etiologic agents, including dermatophytes that specifically colonize keratin-rich substrates. The aim of this work was to investigate mechanical modifications of bovine membranes (used as an in vitro nail model) placed in contact with Trichophyton species. Trichophyton strains were isolated from toenails specimens. The procedure was set up by spreading T. rubrum, T. interdigitale, and T. mentagrophytes strains on Petri dishes with minimal and rich media; after that, bovine membranes were placed in the center. After 27 days, T. interdigitale and T. mentagrophytes significantly reduced the thickness of the colonized membranes, whereas two T. rubrum strains showed the highest degradation limited to the small colonized area. These results were confirmed by SEM images of the colonization profile on membranes. Mechanical analyses performed on membranes were used as an innovative method to evaluate the thickness and structural integrity of membranes variation following fungal colonization. In conclusion, mechanical analyses of substrate may be used as a procedure for the development of a new onychomycosis diagnosis test in order to develop personalized and strain-specific treatment.
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spelling pubmed-96958842022-11-26 Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species Temporiti, Marta Elisabetta Eleonora Guerini, Marta Baiguera, Rebecca Michela Buratti, Simone Desiderio, Anthea Goppa, Lorenzo Perugini, Paola Savino, Elena J Fungi (Basel) Article Onychomycosis is a fungal infection caused by different etiologic agents, including dermatophytes that specifically colonize keratin-rich substrates. The aim of this work was to investigate mechanical modifications of bovine membranes (used as an in vitro nail model) placed in contact with Trichophyton species. Trichophyton strains were isolated from toenails specimens. The procedure was set up by spreading T. rubrum, T. interdigitale, and T. mentagrophytes strains on Petri dishes with minimal and rich media; after that, bovine membranes were placed in the center. After 27 days, T. interdigitale and T. mentagrophytes significantly reduced the thickness of the colonized membranes, whereas two T. rubrum strains showed the highest degradation limited to the small colonized area. These results were confirmed by SEM images of the colonization profile on membranes. Mechanical analyses performed on membranes were used as an innovative method to evaluate the thickness and structural integrity of membranes variation following fungal colonization. In conclusion, mechanical analyses of substrate may be used as a procedure for the development of a new onychomycosis diagnosis test in order to develop personalized and strain-specific treatment. MDPI 2022-10-27 /pmc/articles/PMC9695884/ /pubmed/36354900 http://dx.doi.org/10.3390/jof8111133 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Temporiti, Marta Elisabetta Eleonora
Guerini, Marta
Baiguera, Rebecca Michela
Buratti, Simone
Desiderio, Anthea
Goppa, Lorenzo
Perugini, Paola
Savino, Elena
Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species
title Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species
title_full Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species
title_fullStr Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species
title_full_unstemmed Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species
title_short Efficacy of Bovine Nail Membranes as In Vitro Model for Onychomycosis Infected by Trichophyton Species
title_sort efficacy of bovine nail membranes as in vitro model for onychomycosis infected by trichophyton species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695884/
https://www.ncbi.nlm.nih.gov/pubmed/36354900
http://dx.doi.org/10.3390/jof8111133
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