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Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation
Soybean cyst nematode (SCN, Heterodera glycine) is a serious damaging disease in soybean worldwide, thus resulting in severe yield losses. MicroRNA408 (miR408) is an ancient and highly conserved miRNA involved in regulating plant growth, development, biotic and abiotic stress response. Here, we anal...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695887/ https://www.ncbi.nlm.nih.gov/pubmed/36430501 http://dx.doi.org/10.3390/ijms232214022 |
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author | Feng, Yaxing Qi, Nawei Lei, Piao Wang, Yuanyuan Xuan, Yuanhu Liu, Xiaoyu Fan, Haiyan Chen, Lijie Duan, Yuxi Zhu, Xiaofeng |
author_facet | Feng, Yaxing Qi, Nawei Lei, Piao Wang, Yuanyuan Xuan, Yuanhu Liu, Xiaoyu Fan, Haiyan Chen, Lijie Duan, Yuxi Zhu, Xiaofeng |
author_sort | Feng, Yaxing |
collection | PubMed |
description | Soybean cyst nematode (SCN, Heterodera glycine) is a serious damaging disease in soybean worldwide, thus resulting in severe yield losses. MicroRNA408 (miR408) is an ancient and highly conserved miRNA involved in regulating plant growth, development, biotic and abiotic stress response. Here, we analyzed the evolution of miR408 in plants and verified four miR408 members in Glycine max. In the current research, highly upregulated gma-miR408 expressing was detected during nematode migration and syncytium formation response to soybean cyst nematode infection. Overexpressing and silencing miR408 vectors were transformed to soybean to confirm its potential role in plant and nematode interaction. Significant variations were observed in the MAPK signaling pathway with low OXI1, PR1, and wounding of the overexpressing lines. Overexpressing miR408 could negatively regulate soybean resistance to SCN by suppressing reactive oxygen species accumulation. Conversely, silencing miR408 positively regulates soybean resistance to SCN. Overall, gma-miR408 enhances soybean cyst nematode susceptibility by suppressing reactive oxygen species accumulation. |
format | Online Article Text |
id | pubmed-9695887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96958872022-11-26 Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation Feng, Yaxing Qi, Nawei Lei, Piao Wang, Yuanyuan Xuan, Yuanhu Liu, Xiaoyu Fan, Haiyan Chen, Lijie Duan, Yuxi Zhu, Xiaofeng Int J Mol Sci Article Soybean cyst nematode (SCN, Heterodera glycine) is a serious damaging disease in soybean worldwide, thus resulting in severe yield losses. MicroRNA408 (miR408) is an ancient and highly conserved miRNA involved in regulating plant growth, development, biotic and abiotic stress response. Here, we analyzed the evolution of miR408 in plants and verified four miR408 members in Glycine max. In the current research, highly upregulated gma-miR408 expressing was detected during nematode migration and syncytium formation response to soybean cyst nematode infection. Overexpressing and silencing miR408 vectors were transformed to soybean to confirm its potential role in plant and nematode interaction. Significant variations were observed in the MAPK signaling pathway with low OXI1, PR1, and wounding of the overexpressing lines. Overexpressing miR408 could negatively regulate soybean resistance to SCN by suppressing reactive oxygen species accumulation. Conversely, silencing miR408 positively regulates soybean resistance to SCN. Overall, gma-miR408 enhances soybean cyst nematode susceptibility by suppressing reactive oxygen species accumulation. MDPI 2022-11-14 /pmc/articles/PMC9695887/ /pubmed/36430501 http://dx.doi.org/10.3390/ijms232214022 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Feng, Yaxing Qi, Nawei Lei, Piao Wang, Yuanyuan Xuan, Yuanhu Liu, Xiaoyu Fan, Haiyan Chen, Lijie Duan, Yuxi Zhu, Xiaofeng Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation |
title | Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation |
title_full | Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation |
title_fullStr | Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation |
title_full_unstemmed | Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation |
title_short | Gma-miR408 Enhances Soybean Cyst Nematode Susceptibility by Suppressing Reactive Oxygen Species Accumulation |
title_sort | gma-mir408 enhances soybean cyst nematode susceptibility by suppressing reactive oxygen species accumulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695887/ https://www.ncbi.nlm.nih.gov/pubmed/36430501 http://dx.doi.org/10.3390/ijms232214022 |
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