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Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System

Polypeptide materials have clear secondary structure and biodegradability, which can be further modified and functionalized, so that they can be employed as therapeutic agents in clinical applications. PEGylation of polylysine (PEG-PLL) is a kind of safe and effective nanocarrier that is utilized fo...

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Autores principales: Yu, Bo, Wang, Xiangtao, Ding, Lijuan, Han, Meihua, Guo, Yifei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696163/
https://www.ncbi.nlm.nih.gov/pubmed/36432704
http://dx.doi.org/10.3390/pharmaceutics14112512
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author Yu, Bo
Wang, Xiangtao
Ding, Lijuan
Han, Meihua
Guo, Yifei
author_facet Yu, Bo
Wang, Xiangtao
Ding, Lijuan
Han, Meihua
Guo, Yifei
author_sort Yu, Bo
collection PubMed
description Polypeptide materials have clear secondary structure and biodegradability, which can be further modified and functionalized, so that they can be employed as therapeutic agents in clinical applications. PEGylation of polylysine (PEG-PLL) is a kind of safe and effective nanocarrier that is utilized for gene and drug delivery. However, PEG-PLL needs to be produced through chemical synthesis, which is expensive and difficult to obtain. We hope to simplify the nanocarrier and use hydrophilic natural polylysine (PLL) to develop a high-efficacy delivery system. To evaluate the possibility of PLL as nanocarriers, methotrexate (MTX) is selected as a model drug and PEG-PLL is utilized as control nanocarriers. The experimental results showed that PLL is an ideal polypeptide to prepare MTX-loaded PLL nanoparticles (PLL/MTX NPs). Compared with PEG-PLL as nanocarriers, PLL/MTX NPs showed higher drug-loading content (58.9%) and smaller particle sizes (113.7 nm). Moreover, the shape of PLL/MTX NPs was a unique helical nanorod. The PLL/MTX NPs had good storage stability, media stability, and sustained release effect. Animal research demonstrated that PLL/MTX NPs could improve the anti-tumor activity of MTX, the antitumor efficacy is enhanced 1.9-fold and 1.2-fold compared with MTX injection and PEG-PLL/MTX NPs, respectively. To sum up, natural polymer PLL is an ideal nano drug delivery carrier which has potential clinical applications.
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spelling pubmed-96961632022-11-26 Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System Yu, Bo Wang, Xiangtao Ding, Lijuan Han, Meihua Guo, Yifei Pharmaceutics Article Polypeptide materials have clear secondary structure and biodegradability, which can be further modified and functionalized, so that they can be employed as therapeutic agents in clinical applications. PEGylation of polylysine (PEG-PLL) is a kind of safe and effective nanocarrier that is utilized for gene and drug delivery. However, PEG-PLL needs to be produced through chemical synthesis, which is expensive and difficult to obtain. We hope to simplify the nanocarrier and use hydrophilic natural polylysine (PLL) to develop a high-efficacy delivery system. To evaluate the possibility of PLL as nanocarriers, methotrexate (MTX) is selected as a model drug and PEG-PLL is utilized as control nanocarriers. The experimental results showed that PLL is an ideal polypeptide to prepare MTX-loaded PLL nanoparticles (PLL/MTX NPs). Compared with PEG-PLL as nanocarriers, PLL/MTX NPs showed higher drug-loading content (58.9%) and smaller particle sizes (113.7 nm). Moreover, the shape of PLL/MTX NPs was a unique helical nanorod. The PLL/MTX NPs had good storage stability, media stability, and sustained release effect. Animal research demonstrated that PLL/MTX NPs could improve the anti-tumor activity of MTX, the antitumor efficacy is enhanced 1.9-fold and 1.2-fold compared with MTX injection and PEG-PLL/MTX NPs, respectively. To sum up, natural polymer PLL is an ideal nano drug delivery carrier which has potential clinical applications. MDPI 2022-11-18 /pmc/articles/PMC9696163/ /pubmed/36432704 http://dx.doi.org/10.3390/pharmaceutics14112512 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Bo
Wang, Xiangtao
Ding, Lijuan
Han, Meihua
Guo, Yifei
Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System
title Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System
title_full Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System
title_fullStr Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System
title_full_unstemmed Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System
title_short Hydrophilic Natural Polylysine as Drug Nanocarrier for Preparation of Helical Delivery System
title_sort hydrophilic natural polylysine as drug nanocarrier for preparation of helical delivery system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696163/
https://www.ncbi.nlm.nih.gov/pubmed/36432704
http://dx.doi.org/10.3390/pharmaceutics14112512
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