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Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot

Members of the family of Phosphatidy Ethanolamine-Binding Protein (PEBP) have been shown to be key regulators of the transition of plants from vegetative to reproductive phases. Here, a total of 12 PEBP proteins were identified in the carrot (Daucus carota L.) genome and classified into FT-like (4),...

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Autores principales: Liu, Xing, Zhao, Donghang, Ou, Chenggang, Hao, Weidong, Zhao, Zhiwei, Zhuang, Feiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696379/
https://www.ncbi.nlm.nih.gov/pubmed/36437940
http://dx.doi.org/10.3389/fgene.2022.1047890
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author Liu, Xing
Zhao, Donghang
Ou, Chenggang
Hao, Weidong
Zhao, Zhiwei
Zhuang, Feiyun
author_facet Liu, Xing
Zhao, Donghang
Ou, Chenggang
Hao, Weidong
Zhao, Zhiwei
Zhuang, Feiyun
author_sort Liu, Xing
collection PubMed
description Members of the family of Phosphatidy Ethanolamine-Binding Protein (PEBP) have been shown to be key regulators of the transition of plants from vegetative to reproductive phases. Here, a total of 12 PEBP proteins were identified in the carrot (Daucus carota L.) genome and classified into FT-like (4), TFL1-like (6), and MFT-like 2) subfamilies, that had different lengths (110–267 aa) and were distributed unevenly across seven chromosomes. Moreover, 13 and 31 PEBP proteins were identified in other two Apiaceae species, celery (Apium graveolens L.) and coriander (Coriandrum sativum L.). The phylogenetic and evolutionary results of these PEBP family proteins were obtained based on the protein sequences. In the three Apiaceae species, purifying selection was the main evolutionary force, and WGD, segmental duplication, and dispersed duplication have played key roles in the PEBP family expansion. The expression analysis showed that carrot PEBP genes exhibited relatively broad expression patterns across various tissues. In the period of bolting to flowering, the carrot FT-like subfamily genes were upregulated as positive regulators, and TFL1-like subfamily genes remained at lower expression levels as inhibitors. More interestingly, the members of carrot FT-like genes had different temporal-spatial expression characteristics, suggesting that they have different regulatory functions in the carrot reproductive phase. In summary, this study contributes to our understanding of the PEBP family proteins and provides a foundation for exploring the mechanism of carrot bolting and flowering for the breeding of cultivars with bolting resistance.
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spelling pubmed-96963792022-11-26 Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot Liu, Xing Zhao, Donghang Ou, Chenggang Hao, Weidong Zhao, Zhiwei Zhuang, Feiyun Front Genet Genetics Members of the family of Phosphatidy Ethanolamine-Binding Protein (PEBP) have been shown to be key regulators of the transition of plants from vegetative to reproductive phases. Here, a total of 12 PEBP proteins were identified in the carrot (Daucus carota L.) genome and classified into FT-like (4), TFL1-like (6), and MFT-like 2) subfamilies, that had different lengths (110–267 aa) and were distributed unevenly across seven chromosomes. Moreover, 13 and 31 PEBP proteins were identified in other two Apiaceae species, celery (Apium graveolens L.) and coriander (Coriandrum sativum L.). The phylogenetic and evolutionary results of these PEBP family proteins were obtained based on the protein sequences. In the three Apiaceae species, purifying selection was the main evolutionary force, and WGD, segmental duplication, and dispersed duplication have played key roles in the PEBP family expansion. The expression analysis showed that carrot PEBP genes exhibited relatively broad expression patterns across various tissues. In the period of bolting to flowering, the carrot FT-like subfamily genes were upregulated as positive regulators, and TFL1-like subfamily genes remained at lower expression levels as inhibitors. More interestingly, the members of carrot FT-like genes had different temporal-spatial expression characteristics, suggesting that they have different regulatory functions in the carrot reproductive phase. In summary, this study contributes to our understanding of the PEBP family proteins and provides a foundation for exploring the mechanism of carrot bolting and flowering for the breeding of cultivars with bolting resistance. Frontiers Media S.A. 2022-11-08 /pmc/articles/PMC9696379/ /pubmed/36437940 http://dx.doi.org/10.3389/fgene.2022.1047890 Text en Copyright © 2022 Liu, Zhao, Ou, Hao, Zhao and Zhuang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Liu, Xing
Zhao, Donghang
Ou, Chenggang
Hao, Weidong
Zhao, Zhiwei
Zhuang, Feiyun
Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot
title Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot
title_full Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot
title_fullStr Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot
title_full_unstemmed Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot
title_short Genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot
title_sort genome-wide identification and characterization profile of phosphatidy ethanolamine-binding protein family genes in carrot
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696379/
https://www.ncbi.nlm.nih.gov/pubmed/36437940
http://dx.doi.org/10.3389/fgene.2022.1047890
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