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Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam

Traditional/herbal medicine has gained increasing interests recently, especially in Asian countries such as Vietnam, due to its diverse therapeutic actions. In the treasure of Vietnamese medicinal plants, one of the potential herbs is the roots of Sophora flavescens Ait. (SF, “Kho sam” in Vietnamese...

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Autores principales: Thang, Phan Nguyen Truong, Tran, Viet-Hung, Vu, Tran Anh, Vinh, Nguyen Ngoc, Huynh, Duyen Thi My, Pham, Duy Toan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696406/
https://www.ncbi.nlm.nih.gov/pubmed/36355556
http://dx.doi.org/10.3390/ph15111384
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author Thang, Phan Nguyen Truong
Tran, Viet-Hung
Vu, Tran Anh
Vinh, Nguyen Ngoc
Huynh, Duyen Thi My
Pham, Duy Toan
author_facet Thang, Phan Nguyen Truong
Tran, Viet-Hung
Vu, Tran Anh
Vinh, Nguyen Ngoc
Huynh, Duyen Thi My
Pham, Duy Toan
author_sort Thang, Phan Nguyen Truong
collection PubMed
description Traditional/herbal medicine has gained increasing interests recently, especially in Asian countries such as Vietnam, due to its diverse therapeutic actions. In the treasure of Vietnamese medicinal plants, one of the potential herbs is the roots of Sophora flavescens Ait. (SF, “Kho sam” in Vietnamese). However, limited information has been reported on the Vietnamese SF compositions and their respective alkaloids’ anti-acetylcholinesterase action. Thus, this study investigated the extractions, isolations, identifications, and in-vitro antioxidant, cytotoxicity, and acetylcholinesterase inhibitory activities, of the SF root extracts and their purified alkaloid compounds. To this end, four pure compounds were successfully isolated, purity-tested by HPLC, and structurally identified by spectroscopic techniques of FTIR, MS, and NMR. These compounds, confirmed to be oxysophocarpine, oxymatrine, matrine, and sophoridine, were then determined their therapeutic actions. The SF extracts and the compounds did not possess significant antioxidant activity using the DPPH and MDA assays, and cytotoxicity action using the MTT assay on the MDA-MB-231 breast cancer cell line. On the other hand, the SF total extract yielded a moderate acetylcholinesterase inhibition effect, with an IC50 of 0.1077 ± 0.0023 mg/mL. In summary, the SF extract demonstrated potential effects as an anti-acetylcholinesterase agent and could be further researched to become a pharmaceutical product for diseases related to acetylcholine deficiency, such as dementia.
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spelling pubmed-96964062022-11-26 Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam Thang, Phan Nguyen Truong Tran, Viet-Hung Vu, Tran Anh Vinh, Nguyen Ngoc Huynh, Duyen Thi My Pham, Duy Toan Pharmaceuticals (Basel) Article Traditional/herbal medicine has gained increasing interests recently, especially in Asian countries such as Vietnam, due to its diverse therapeutic actions. In the treasure of Vietnamese medicinal plants, one of the potential herbs is the roots of Sophora flavescens Ait. (SF, “Kho sam” in Vietnamese). However, limited information has been reported on the Vietnamese SF compositions and their respective alkaloids’ anti-acetylcholinesterase action. Thus, this study investigated the extractions, isolations, identifications, and in-vitro antioxidant, cytotoxicity, and acetylcholinesterase inhibitory activities, of the SF root extracts and their purified alkaloid compounds. To this end, four pure compounds were successfully isolated, purity-tested by HPLC, and structurally identified by spectroscopic techniques of FTIR, MS, and NMR. These compounds, confirmed to be oxysophocarpine, oxymatrine, matrine, and sophoridine, were then determined their therapeutic actions. The SF extracts and the compounds did not possess significant antioxidant activity using the DPPH and MDA assays, and cytotoxicity action using the MTT assay on the MDA-MB-231 breast cancer cell line. On the other hand, the SF total extract yielded a moderate acetylcholinesterase inhibition effect, with an IC50 of 0.1077 ± 0.0023 mg/mL. In summary, the SF extract demonstrated potential effects as an anti-acetylcholinesterase agent and could be further researched to become a pharmaceutical product for diseases related to acetylcholine deficiency, such as dementia. MDPI 2022-11-10 /pmc/articles/PMC9696406/ /pubmed/36355556 http://dx.doi.org/10.3390/ph15111384 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thang, Phan Nguyen Truong
Tran, Viet-Hung
Vu, Tran Anh
Vinh, Nguyen Ngoc
Huynh, Duyen Thi My
Pham, Duy Toan
Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam
title Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam
title_full Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam
title_fullStr Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam
title_full_unstemmed Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam
title_short Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam
title_sort determination of antioxidant, cytotoxicity, and acetylcholinesterase inhibitory activities of alkaloids isolated from sophora flavescens ait. grown in dak nong, vietnam
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696406/
https://www.ncbi.nlm.nih.gov/pubmed/36355556
http://dx.doi.org/10.3390/ph15111384
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