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Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment

Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old Arabidopsis thaliana (L.) Heynh. seedlings grown from surfac...

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Autores principales: Waskow, Alexandra, Guihur, Anthony, Howling, Alan, Furno, Ivo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696580/
https://www.ncbi.nlm.nih.gov/pubmed/36362977
http://dx.doi.org/10.3390/life12111822
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author Waskow, Alexandra
Guihur, Anthony
Howling, Alan
Furno, Ivo
author_facet Waskow, Alexandra
Guihur, Anthony
Howling, Alan
Furno, Ivo
author_sort Waskow, Alexandra
collection PubMed
description Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old Arabidopsis thaliana (L.) Heynh. seedlings grown from surface dielectric barrier discharge plasma-treated seeds. In a previous study, seeds were analyzed 6 days after plasma exposure and a plant stress and defense response was observed. Here, we performed a pathway analysis on differentially expressed genes and our results revealed again an increased expression of plant stress and defense, specifically glucosinolate pathway-related compounds. The main difference was that a different part of the plant defense response changed at 7 days, which was not previously observed at 6 days. With a 24-h delayed extraction time point, the glucosinolates were selectively broken down into nitriles among all of the glucosinolates catabolic products. Although information about nitriles is limited, it protects plants against biotic stresses and has variable toxicity depending on the interacting organism. More work needs to be performed to better understand which plasma seed treatment parameters affect plant defense; however, these preliminary findings suggest that an optimized plasma treatment could be used to elicit a plant defense response.
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spelling pubmed-96965802022-11-26 Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment Waskow, Alexandra Guihur, Anthony Howling, Alan Furno, Ivo Life (Basel) Article Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old Arabidopsis thaliana (L.) Heynh. seedlings grown from surface dielectric barrier discharge plasma-treated seeds. In a previous study, seeds were analyzed 6 days after plasma exposure and a plant stress and defense response was observed. Here, we performed a pathway analysis on differentially expressed genes and our results revealed again an increased expression of plant stress and defense, specifically glucosinolate pathway-related compounds. The main difference was that a different part of the plant defense response changed at 7 days, which was not previously observed at 6 days. With a 24-h delayed extraction time point, the glucosinolates were selectively broken down into nitriles among all of the glucosinolates catabolic products. Although information about nitriles is limited, it protects plants against biotic stresses and has variable toxicity depending on the interacting organism. More work needs to be performed to better understand which plasma seed treatment parameters affect plant defense; however, these preliminary findings suggest that an optimized plasma treatment could be used to elicit a plant defense response. MDPI 2022-11-08 /pmc/articles/PMC9696580/ /pubmed/36362977 http://dx.doi.org/10.3390/life12111822 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Waskow, Alexandra
Guihur, Anthony
Howling, Alan
Furno, Ivo
Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment
title Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment
title_full Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment
title_fullStr Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment
title_full_unstemmed Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment
title_short Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment
title_sort catabolism of glucosinolates into nitriles revealed by rna sequencing of arabidopsis thaliana seedlings after non-thermal plasma-seed treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696580/
https://www.ncbi.nlm.nih.gov/pubmed/36362977
http://dx.doi.org/10.3390/life12111822
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