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Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS
A number of studies have suggested that human herpesvirus 6A (HHV-6A) may play a role in multiple sclerosis (MS). Three possible hypotheses have been investigated: (1) U24 from HHV-6A (U24-6A) mimics myelin basic protein (MBP) through analogous phosphorylation and interaction with Fyn-SH3; (2) U24-6...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696605/ https://www.ncbi.nlm.nih.gov/pubmed/36366483 http://dx.doi.org/10.3390/v14112384 |
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author | Pi, Keng-Shuo Bortolotti, Daria Sang, Yurou Schiuma, Giovanna Beltrami, Silvia Rizzo, Sabrina Bortoluzzi, Alessandra Baldi, Eleonora Creagh, A. Louise Haynes, Charles A. Rizzo, Roberta Straus, Suzana K. |
author_facet | Pi, Keng-Shuo Bortolotti, Daria Sang, Yurou Schiuma, Giovanna Beltrami, Silvia Rizzo, Sabrina Bortoluzzi, Alessandra Baldi, Eleonora Creagh, A. Louise Haynes, Charles A. Rizzo, Roberta Straus, Suzana K. |
author_sort | Pi, Keng-Shuo |
collection | PubMed |
description | A number of studies have suggested that human herpesvirus 6A (HHV-6A) may play a role in multiple sclerosis (MS). Three possible hypotheses have been investigated: (1) U24 from HHV-6A (U24-6A) mimics myelin basic protein (MBP) through analogous phosphorylation and interaction with Fyn-SH3; (2) U24-6A affects endocytic recycling by binding human neural precursor cell (NPC) expressed developmentally down-regulated protein 4-like WW3* domain (hNedd4L-WW3*); and (3) MS patients who express Killer Cell Immunoglobulin Like Receptor 2DL2 (KIR2DL2) on natural killer (NK) cells are more susceptible to HHV-6 infection. In this contribution, we examined the validity of these propositions by investigating the interactions of U24 from HHV-6B (U24-6B), a variant less commonly linked to MS, with Fyn-SH3 and hNedd4L-WW3* using heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) titrations and isothermal titration calorimetry (ITC). In addition, the importance of phosphorylation and the specific role of U24 in NK cell activation in MS patients were examined. Overall, the findings allowed us to shed light into the models linking HHV-6 to MS and the involvement of U24. |
format | Online Article Text |
id | pubmed-9696605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96966052022-11-26 Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS Pi, Keng-Shuo Bortolotti, Daria Sang, Yurou Schiuma, Giovanna Beltrami, Silvia Rizzo, Sabrina Bortoluzzi, Alessandra Baldi, Eleonora Creagh, A. Louise Haynes, Charles A. Rizzo, Roberta Straus, Suzana K. Viruses Article A number of studies have suggested that human herpesvirus 6A (HHV-6A) may play a role in multiple sclerosis (MS). Three possible hypotheses have been investigated: (1) U24 from HHV-6A (U24-6A) mimics myelin basic protein (MBP) through analogous phosphorylation and interaction with Fyn-SH3; (2) U24-6A affects endocytic recycling by binding human neural precursor cell (NPC) expressed developmentally down-regulated protein 4-like WW3* domain (hNedd4L-WW3*); and (3) MS patients who express Killer Cell Immunoglobulin Like Receptor 2DL2 (KIR2DL2) on natural killer (NK) cells are more susceptible to HHV-6 infection. In this contribution, we examined the validity of these propositions by investigating the interactions of U24 from HHV-6B (U24-6B), a variant less commonly linked to MS, with Fyn-SH3 and hNedd4L-WW3* using heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) titrations and isothermal titration calorimetry (ITC). In addition, the importance of phosphorylation and the specific role of U24 in NK cell activation in MS patients were examined. Overall, the findings allowed us to shed light into the models linking HHV-6 to MS and the involvement of U24. MDPI 2022-10-28 /pmc/articles/PMC9696605/ /pubmed/36366483 http://dx.doi.org/10.3390/v14112384 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pi, Keng-Shuo Bortolotti, Daria Sang, Yurou Schiuma, Giovanna Beltrami, Silvia Rizzo, Sabrina Bortoluzzi, Alessandra Baldi, Eleonora Creagh, A. Louise Haynes, Charles A. Rizzo, Roberta Straus, Suzana K. Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS |
title | Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS |
title_full | Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS |
title_fullStr | Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS |
title_full_unstemmed | Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS |
title_short | Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its Potential Role in MS |
title_sort | studying the interactions of u24 from hhv-6 in order to further elucidate its potential role in ms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696605/ https://www.ncbi.nlm.nih.gov/pubmed/36366483 http://dx.doi.org/10.3390/v14112384 |
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