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The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin

The potential of plant lectins (carbohydrate-binding proteins) for the treatment of neurological disorders such as anxiety and depression has started to be reported in the last few years. Schinus terebinthifolia leaves contain a lectin called SteLL, which has displayed antimicrobial, immunomodulator...

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Autores principales: de Lima, Bárbara Raíssa Ferreira, Patriota, Leydianne Leite de Siqueira, Marinho, Amanda de Oliveira, da Costa, Jainaldo Alves, Napoleão, Thiago Henrique, da Rosa, Michelle Melgarejo, Paiva, Patrícia Maria Guedes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696607/
https://www.ncbi.nlm.nih.gov/pubmed/36355536
http://dx.doi.org/10.3390/ph15111364
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author de Lima, Bárbara Raíssa Ferreira
Patriota, Leydianne Leite de Siqueira
Marinho, Amanda de Oliveira
da Costa, Jainaldo Alves
Napoleão, Thiago Henrique
da Rosa, Michelle Melgarejo
Paiva, Patrícia Maria Guedes
author_facet de Lima, Bárbara Raíssa Ferreira
Patriota, Leydianne Leite de Siqueira
Marinho, Amanda de Oliveira
da Costa, Jainaldo Alves
Napoleão, Thiago Henrique
da Rosa, Michelle Melgarejo
Paiva, Patrícia Maria Guedes
author_sort de Lima, Bárbara Raíssa Ferreira
collection PubMed
description The potential of plant lectins (carbohydrate-binding proteins) for the treatment of neurological disorders such as anxiety and depression has started to be reported in the last few years. Schinus terebinthifolia leaves contain a lectin called SteLL, which has displayed antimicrobial, immunomodulatory, antitumor, and analgesic activities. However, the effects of SteLL on the Central Nervous System (CNS) have not yet been determined. In this study, we investigated the in vivo anxiolytic effect of SteLL in mice using the open field (OF) and elevated plus maze (EPM) tests. In the OF, SteLL (1, 2, and 4 mg/kg, i.p.) did not interfere with the number of crossings but significantly reduced the number of rearings. In the EPM, SteLL 4 mg/kg and the combination SteLL (1 mg/kg) plus diazepam (1 mg/kg) significantly increased the time spent in the open arms while reducing the time spent in the closed arms. The anxiolytic effect of SteLL did not seem to be dependent on the carbohydrate-binding domain of the lectin. Nevertheless, the SteLL effect in the EPM was reversed by the pretreatment with the pharmacological antagonists of the α2-adrenoceptor, 5-HT2A/2C serotonin receptor, and the D1 dopamine receptor. Overall, our results suggest that the anxiolytic effect of SteLL is dependent on the monoaminergic signaling cascade.
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spelling pubmed-96966072022-11-26 The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin de Lima, Bárbara Raíssa Ferreira Patriota, Leydianne Leite de Siqueira Marinho, Amanda de Oliveira da Costa, Jainaldo Alves Napoleão, Thiago Henrique da Rosa, Michelle Melgarejo Paiva, Patrícia Maria Guedes Pharmaceuticals (Basel) Article The potential of plant lectins (carbohydrate-binding proteins) for the treatment of neurological disorders such as anxiety and depression has started to be reported in the last few years. Schinus terebinthifolia leaves contain a lectin called SteLL, which has displayed antimicrobial, immunomodulatory, antitumor, and analgesic activities. However, the effects of SteLL on the Central Nervous System (CNS) have not yet been determined. In this study, we investigated the in vivo anxiolytic effect of SteLL in mice using the open field (OF) and elevated plus maze (EPM) tests. In the OF, SteLL (1, 2, and 4 mg/kg, i.p.) did not interfere with the number of crossings but significantly reduced the number of rearings. In the EPM, SteLL 4 mg/kg and the combination SteLL (1 mg/kg) plus diazepam (1 mg/kg) significantly increased the time spent in the open arms while reducing the time spent in the closed arms. The anxiolytic effect of SteLL did not seem to be dependent on the carbohydrate-binding domain of the lectin. Nevertheless, the SteLL effect in the EPM was reversed by the pretreatment with the pharmacological antagonists of the α2-adrenoceptor, 5-HT2A/2C serotonin receptor, and the D1 dopamine receptor. Overall, our results suggest that the anxiolytic effect of SteLL is dependent on the monoaminergic signaling cascade. MDPI 2022-11-07 /pmc/articles/PMC9696607/ /pubmed/36355536 http://dx.doi.org/10.3390/ph15111364 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Lima, Bárbara Raíssa Ferreira
Patriota, Leydianne Leite de Siqueira
Marinho, Amanda de Oliveira
da Costa, Jainaldo Alves
Napoleão, Thiago Henrique
da Rosa, Michelle Melgarejo
Paiva, Patrícia Maria Guedes
The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin
title The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin
title_full The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin
title_fullStr The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin
title_full_unstemmed The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin
title_short The Anxiolytic Activity of Schinus terebinthifolia Leaf Lectin (SteLL) Is Dependent on Monoaminergic Signaling although Independent of the Carbohydrate-Binding Domain of the Lectin
title_sort anxiolytic activity of schinus terebinthifolia leaf lectin (stell) is dependent on monoaminergic signaling although independent of the carbohydrate-binding domain of the lectin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696607/
https://www.ncbi.nlm.nih.gov/pubmed/36355536
http://dx.doi.org/10.3390/ph15111364
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