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Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus
Glioblastoma multiforme (GBM) accounts for almost half of all primary malignant brain tumors in adults and has a poor prognosis. Here we demonstrated the oncolytic potential of the L-16 vaccine strain of measles virus (MV) against primary human GBM cells and characterized the genetic patterns that d...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696624/ https://www.ncbi.nlm.nih.gov/pubmed/36366531 http://dx.doi.org/10.3390/v14112433 |
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author | Ammour, Yulia Susova, Olga Krasnov, George Nikolaeva, Eugenia Varachev, Vyacheslav Schetinina, Yulia Gavrilova, Marina Mitrofanov, Alexey Poletaeva, Anna Bekyashev, Ali Faizuloev, Evgeny Zverev, Vitaly V. Svitich, Oxana A. Nasedkina, Tatiana V. |
author_facet | Ammour, Yulia Susova, Olga Krasnov, George Nikolaeva, Eugenia Varachev, Vyacheslav Schetinina, Yulia Gavrilova, Marina Mitrofanov, Alexey Poletaeva, Anna Bekyashev, Ali Faizuloev, Evgeny Zverev, Vitaly V. Svitich, Oxana A. Nasedkina, Tatiana V. |
author_sort | Ammour, Yulia |
collection | PubMed |
description | Glioblastoma multiforme (GBM) accounts for almost half of all primary malignant brain tumors in adults and has a poor prognosis. Here we demonstrated the oncolytic potential of the L-16 vaccine strain of measles virus (MV) against primary human GBM cells and characterized the genetic patterns that determine the sensitivity of primary human GBM cells to oncolytic therapy. MV replicated in all GBM cells, and seven out of eight cell lines underwent complete or partial oncolysis. RNA-Seq analysis identified about 1200 differentially expressed genes (FDR < 0.05) with at least two-fold expression level change between MV-infected and uninfected cells. Among them, the most significant upregulation was observed for interferon response, apoptosis and cytokine signaling. One out of eight GBM cell lines was defective in type I interferon production and, thus, in the post-interferon response, other cells lacked expression of different cellular defense factors. Thus, none of the cell lines displayed induction of the total gene set necessary for effective inhibition of MV replication. In the resistant cells, we detected aberrant expression of metalloproteinase genes, particularly MMP3. Thus, such genes could be considered intriguing candidates for further study of factors responsible for cell sensitivity and resistance to L-16 MV infection. |
format | Online Article Text |
id | pubmed-9696624 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96966242022-11-26 Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus Ammour, Yulia Susova, Olga Krasnov, George Nikolaeva, Eugenia Varachev, Vyacheslav Schetinina, Yulia Gavrilova, Marina Mitrofanov, Alexey Poletaeva, Anna Bekyashev, Ali Faizuloev, Evgeny Zverev, Vitaly V. Svitich, Oxana A. Nasedkina, Tatiana V. Viruses Article Glioblastoma multiforme (GBM) accounts for almost half of all primary malignant brain tumors in adults and has a poor prognosis. Here we demonstrated the oncolytic potential of the L-16 vaccine strain of measles virus (MV) against primary human GBM cells and characterized the genetic patterns that determine the sensitivity of primary human GBM cells to oncolytic therapy. MV replicated in all GBM cells, and seven out of eight cell lines underwent complete or partial oncolysis. RNA-Seq analysis identified about 1200 differentially expressed genes (FDR < 0.05) with at least two-fold expression level change between MV-infected and uninfected cells. Among them, the most significant upregulation was observed for interferon response, apoptosis and cytokine signaling. One out of eight GBM cell lines was defective in type I interferon production and, thus, in the post-interferon response, other cells lacked expression of different cellular defense factors. Thus, none of the cell lines displayed induction of the total gene set necessary for effective inhibition of MV replication. In the resistant cells, we detected aberrant expression of metalloproteinase genes, particularly MMP3. Thus, such genes could be considered intriguing candidates for further study of factors responsible for cell sensitivity and resistance to L-16 MV infection. MDPI 2022-11-02 /pmc/articles/PMC9696624/ /pubmed/36366531 http://dx.doi.org/10.3390/v14112433 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ammour, Yulia Susova, Olga Krasnov, George Nikolaeva, Eugenia Varachev, Vyacheslav Schetinina, Yulia Gavrilova, Marina Mitrofanov, Alexey Poletaeva, Anna Bekyashev, Ali Faizuloev, Evgeny Zverev, Vitaly V. Svitich, Oxana A. Nasedkina, Tatiana V. Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus |
title | Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus |
title_full | Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus |
title_fullStr | Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus |
title_full_unstemmed | Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus |
title_short | Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus |
title_sort | transcriptome analysis of human glioblastoma cells susceptible to infection with the leningrad-16 vaccine strain of measles virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696624/ https://www.ncbi.nlm.nih.gov/pubmed/36366531 http://dx.doi.org/10.3390/v14112433 |
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