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Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine

Solid dose vaccine formulation and delivery systems offer potential advantages over traditional liquid vaccine formulations. In addition to enhanced thermostability, needle-free delivery of unit solid dose injectable (USDI) vaccines offers safe, rapid, and error-free administration, with applicabili...

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Autores principales: Hayhurst, Ellie, Rose, Emily, Pedrera, Miriam, Edwards, Jane C., Kotynska, Natalia, Grainger, Daisy, Sadigh, Yashar, Flannery, John, Bonnet, Ludo, Ritwik, Ritwik, Dulal, Pawan, Howard, M. Keith, Graham, Simon P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696641/
https://www.ncbi.nlm.nih.gov/pubmed/36366345
http://dx.doi.org/10.3390/vaccines10111836
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author Hayhurst, Ellie
Rose, Emily
Pedrera, Miriam
Edwards, Jane C.
Kotynska, Natalia
Grainger, Daisy
Sadigh, Yashar
Flannery, John
Bonnet, Ludo
Ritwik, Ritwik
Dulal, Pawan
Howard, M. Keith
Graham, Simon P.
author_facet Hayhurst, Ellie
Rose, Emily
Pedrera, Miriam
Edwards, Jane C.
Kotynska, Natalia
Grainger, Daisy
Sadigh, Yashar
Flannery, John
Bonnet, Ludo
Ritwik, Ritwik
Dulal, Pawan
Howard, M. Keith
Graham, Simon P.
author_sort Hayhurst, Ellie
collection PubMed
description Solid dose vaccine formulation and delivery systems offer potential advantages over traditional liquid vaccine formulations. In addition to enhanced thermostability, needle-free delivery of unit solid dose injectable (USDI) vaccines offers safe, rapid, and error-free administration, with applicability to both human and animal health. Solid dose formulation technologies can be adapted for delivery of different vaccine formats including live attenuated vaccines, which remain the ‘gold standard’ for many disease targets. Porcine reproductive and respiratory syndrome viruses (PRRSV) cause one of the most economically important diseases affecting the global pig industry. Despite several shortcomings, live attenuated vaccines are widely used to control PRRSV. We optimised a freeze-dried USDI formulation of live attenuated PRRSV-1, which fully retained infectious titre, and evaluated its immunogenicity in comparison to virus delivered in liquid suspension via intramuscular and subcutaneous needle inoculation. Pigs vaccinated with the USDI formulation displayed vaccine viraemia, and PRRSV-specific antibody and T cell responses comparable to animals immunised with the liquid vaccine. The USDI vaccine formulation was stable for at least 6 months when stored refrigerated. These data demonstrate the potential for a solid dose vaccine delivery system as an alternative to conventional needle-syringe delivery of live attenuated PRRSV vaccines.
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spelling pubmed-96966412022-11-26 Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine Hayhurst, Ellie Rose, Emily Pedrera, Miriam Edwards, Jane C. Kotynska, Natalia Grainger, Daisy Sadigh, Yashar Flannery, John Bonnet, Ludo Ritwik, Ritwik Dulal, Pawan Howard, M. Keith Graham, Simon P. Vaccines (Basel) Article Solid dose vaccine formulation and delivery systems offer potential advantages over traditional liquid vaccine formulations. In addition to enhanced thermostability, needle-free delivery of unit solid dose injectable (USDI) vaccines offers safe, rapid, and error-free administration, with applicability to both human and animal health. Solid dose formulation technologies can be adapted for delivery of different vaccine formats including live attenuated vaccines, which remain the ‘gold standard’ for many disease targets. Porcine reproductive and respiratory syndrome viruses (PRRSV) cause one of the most economically important diseases affecting the global pig industry. Despite several shortcomings, live attenuated vaccines are widely used to control PRRSV. We optimised a freeze-dried USDI formulation of live attenuated PRRSV-1, which fully retained infectious titre, and evaluated its immunogenicity in comparison to virus delivered in liquid suspension via intramuscular and subcutaneous needle inoculation. Pigs vaccinated with the USDI formulation displayed vaccine viraemia, and PRRSV-specific antibody and T cell responses comparable to animals immunised with the liquid vaccine. The USDI vaccine formulation was stable for at least 6 months when stored refrigerated. These data demonstrate the potential for a solid dose vaccine delivery system as an alternative to conventional needle-syringe delivery of live attenuated PRRSV vaccines. MDPI 2022-10-30 /pmc/articles/PMC9696641/ /pubmed/36366345 http://dx.doi.org/10.3390/vaccines10111836 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hayhurst, Ellie
Rose, Emily
Pedrera, Miriam
Edwards, Jane C.
Kotynska, Natalia
Grainger, Daisy
Sadigh, Yashar
Flannery, John
Bonnet, Ludo
Ritwik, Ritwik
Dulal, Pawan
Howard, M. Keith
Graham, Simon P.
Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
title Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
title_full Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
title_fullStr Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
title_full_unstemmed Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
title_short Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
title_sort evaluation of the delivery of a live attenuated porcine reproductive and respiratory syndrome virus as a unit solid dose injectable vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696641/
https://www.ncbi.nlm.nih.gov/pubmed/36366345
http://dx.doi.org/10.3390/vaccines10111836
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