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The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains

Deguelia nitidula (Benth.) A.M.G.Azevedo & R.A.Camargo (Fabaceae) is an herbaceous plant distributed in the Brazilian Amazon, and it is called “raiz do sol” (sun roots). On Marajó Island, quilombola communities use its prepared roots to treat skin diseases commonly caused by fungi, viruses, and...

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Autores principales: Nogueira-Lima, Suzana Helena Campelo, Gomes, Paulo Wender P., Navegantes-Lima, Kely C., Reis, José Diogo E., Carvalho, Alice Rhelly Veloso, Pamplona, Sônia das Graças Santa R., Muribeca, Abraão de Jesus B., da Silva, Milton N., Monteiro, Marta C., e Silva, Consuelo Yumiko Yoshioka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696647/
https://www.ncbi.nlm.nih.gov/pubmed/36355166
http://dx.doi.org/10.3390/metabo12111083
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author Nogueira-Lima, Suzana Helena Campelo
Gomes, Paulo Wender P.
Navegantes-Lima, Kely C.
Reis, José Diogo E.
Carvalho, Alice Rhelly Veloso
Pamplona, Sônia das Graças Santa R.
Muribeca, Abraão de Jesus B.
da Silva, Milton N.
Monteiro, Marta C.
e Silva, Consuelo Yumiko Yoshioka
author_facet Nogueira-Lima, Suzana Helena Campelo
Gomes, Paulo Wender P.
Navegantes-Lima, Kely C.
Reis, José Diogo E.
Carvalho, Alice Rhelly Veloso
Pamplona, Sônia das Graças Santa R.
Muribeca, Abraão de Jesus B.
da Silva, Milton N.
Monteiro, Marta C.
e Silva, Consuelo Yumiko Yoshioka
author_sort Nogueira-Lima, Suzana Helena Campelo
collection PubMed
description Deguelia nitidula (Benth.) A.M.G.Azevedo & R.A.Camargo (Fabaceae) is an herbaceous plant distributed in the Brazilian Amazon, and it is called “raiz do sol” (sun roots). On Marajó Island, quilombola communities use its prepared roots to treat skin diseases commonly caused by fungi, viruses, and bacteria. Thus, in this study, the extract, and its fractions from D. nitidula roots were used to perform in vitro cytotoxic and antibacterial assays against Staphylococcus aureus strains. Thereafter, liquid chromatography–mass spectrometry (LC–MS) was used for the metabolite annotation process. The ethanolic extract of D. nitidula roots show significant bactericidal activity against S. aureus with IC(50) 82 μg.mL(−1) and a selectivity index (SI) of 21.35. Furthermore, the SREFr2 and SREFr3 fractions show a potent bactericidal activity, i.e., MIC of 46.8 μg.mL(−1) for both, and MBC of 375 and 93.7 μg.mL(−1), respectively. As showcased, SREFr3 shows safe and effective antibacterial activity mainly in respect to the excellent selectivity index (SI = 82.06). On the other hand, SREFr2 shows low selectivity (SI = 6.8), which characterizes it as not safe for therapeutic use. Otherwise, due to a limited amount of reference MS(2) spectra in public libraries, up to now, it was not possible to perform a complete metabolite annotation. Despite that, our antibacterial results for SREFr3 and correlated substructures of amino acid derivatives show that the roots of D. nitidula are a natural source of specialized metabolites, which can be isolated in the future, and then used as a support for further bio-guided research, as well as natural drug development.
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spelling pubmed-96966472022-11-26 The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains Nogueira-Lima, Suzana Helena Campelo Gomes, Paulo Wender P. Navegantes-Lima, Kely C. Reis, José Diogo E. Carvalho, Alice Rhelly Veloso Pamplona, Sônia das Graças Santa R. Muribeca, Abraão de Jesus B. da Silva, Milton N. Monteiro, Marta C. e Silva, Consuelo Yumiko Yoshioka Metabolites Article Deguelia nitidula (Benth.) A.M.G.Azevedo & R.A.Camargo (Fabaceae) is an herbaceous plant distributed in the Brazilian Amazon, and it is called “raiz do sol” (sun roots). On Marajó Island, quilombola communities use its prepared roots to treat skin diseases commonly caused by fungi, viruses, and bacteria. Thus, in this study, the extract, and its fractions from D. nitidula roots were used to perform in vitro cytotoxic and antibacterial assays against Staphylococcus aureus strains. Thereafter, liquid chromatography–mass spectrometry (LC–MS) was used for the metabolite annotation process. The ethanolic extract of D. nitidula roots show significant bactericidal activity against S. aureus with IC(50) 82 μg.mL(−1) and a selectivity index (SI) of 21.35. Furthermore, the SREFr2 and SREFr3 fractions show a potent bactericidal activity, i.e., MIC of 46.8 μg.mL(−1) for both, and MBC of 375 and 93.7 μg.mL(−1), respectively. As showcased, SREFr3 shows safe and effective antibacterial activity mainly in respect to the excellent selectivity index (SI = 82.06). On the other hand, SREFr2 shows low selectivity (SI = 6.8), which characterizes it as not safe for therapeutic use. Otherwise, due to a limited amount of reference MS(2) spectra in public libraries, up to now, it was not possible to perform a complete metabolite annotation. Despite that, our antibacterial results for SREFr3 and correlated substructures of amino acid derivatives show that the roots of D. nitidula are a natural source of specialized metabolites, which can be isolated in the future, and then used as a support for further bio-guided research, as well as natural drug development. MDPI 2022-11-08 /pmc/articles/PMC9696647/ /pubmed/36355166 http://dx.doi.org/10.3390/metabo12111083 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nogueira-Lima, Suzana Helena Campelo
Gomes, Paulo Wender P.
Navegantes-Lima, Kely C.
Reis, José Diogo E.
Carvalho, Alice Rhelly Veloso
Pamplona, Sônia das Graças Santa R.
Muribeca, Abraão de Jesus B.
da Silva, Milton N.
Monteiro, Marta C.
e Silva, Consuelo Yumiko Yoshioka
The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains
title The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains
title_full The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains
title_fullStr The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains
title_full_unstemmed The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains
title_short The Roots of Deguelia nitidula as a Natural Antibacterial Source against Staphylococcus aureus Strains
title_sort roots of deguelia nitidula as a natural antibacterial source against staphylococcus aureus strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696647/
https://www.ncbi.nlm.nih.gov/pubmed/36355166
http://dx.doi.org/10.3390/metabo12111083
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