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Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera
A detailed examination of a unique molecular family, restricted to the Callyspongia genus, in a molecular network obtained from an in-house Haplosclerida marine sponge collection (including Haliclona, Callyspongia, Xestospongia, and Petrosia species) led to the discovery of subarmigerides, a series...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696656/ https://www.ncbi.nlm.nih.gov/pubmed/36354996 http://dx.doi.org/10.3390/md20110673 |
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author | Castaldi, Andrea Teta, Roberta Esposito, Germana Beniddir, Mehdi A. De Voogd, Nicole J. Duperron, Sébastien Costantino, Valeria Bourguet-Kondracki, Marie-Lise |
author_facet | Castaldi, Andrea Teta, Roberta Esposito, Germana Beniddir, Mehdi A. De Voogd, Nicole J. Duperron, Sébastien Costantino, Valeria Bourguet-Kondracki, Marie-Lise |
author_sort | Castaldi, Andrea |
collection | PubMed |
description | A detailed examination of a unique molecular family, restricted to the Callyspongia genus, in a molecular network obtained from an in-house Haplosclerida marine sponge collection (including Haliclona, Callyspongia, Xestospongia, and Petrosia species) led to the discovery of subarmigerides, a series of rare linear peptides from Callyspongia subarmigera, a genus mainly known for polyacetylenes and lipids. The structure of the sole isolated peptide, subarmigeride A (1) was elucidated through extensive 1D and 2D NMR spectroscopy, HRMS/MS, and Marfey’s method to assign its absolute configuration. The putative structures of seven additional linear peptides were proposed by an analysis of their respective MS/MS spectra and a comparison of their fragmentation patterns with the heptapeptide 1. Surprisingly, several structurally related analogues of subarmigeride A (1) occurred in one distinct cluster from the molecular network of the cyanobacteria strains of the Guadeloupe mangroves, suggesting that the true producer of this peptide family might be the microbial sponge-associated community, i.e., the sponge-associated cyanobacteria. |
format | Online Article Text |
id | pubmed-9696656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96966562022-11-26 Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera Castaldi, Andrea Teta, Roberta Esposito, Germana Beniddir, Mehdi A. De Voogd, Nicole J. Duperron, Sébastien Costantino, Valeria Bourguet-Kondracki, Marie-Lise Mar Drugs Article A detailed examination of a unique molecular family, restricted to the Callyspongia genus, in a molecular network obtained from an in-house Haplosclerida marine sponge collection (including Haliclona, Callyspongia, Xestospongia, and Petrosia species) led to the discovery of subarmigerides, a series of rare linear peptides from Callyspongia subarmigera, a genus mainly known for polyacetylenes and lipids. The structure of the sole isolated peptide, subarmigeride A (1) was elucidated through extensive 1D and 2D NMR spectroscopy, HRMS/MS, and Marfey’s method to assign its absolute configuration. The putative structures of seven additional linear peptides were proposed by an analysis of their respective MS/MS spectra and a comparison of their fragmentation patterns with the heptapeptide 1. Surprisingly, several structurally related analogues of subarmigeride A (1) occurred in one distinct cluster from the molecular network of the cyanobacteria strains of the Guadeloupe mangroves, suggesting that the true producer of this peptide family might be the microbial sponge-associated community, i.e., the sponge-associated cyanobacteria. MDPI 2022-10-27 /pmc/articles/PMC9696656/ /pubmed/36354996 http://dx.doi.org/10.3390/md20110673 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Castaldi, Andrea Teta, Roberta Esposito, Germana Beniddir, Mehdi A. De Voogd, Nicole J. Duperron, Sébastien Costantino, Valeria Bourguet-Kondracki, Marie-Lise Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera |
title | Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera |
title_full | Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera |
title_fullStr | Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera |
title_full_unstemmed | Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera |
title_short | Computational Metabolomics Tools Reveal Subarmigerides, Unprecedented Linear Peptides from the Marine Sponge Holobiont Callyspongia subarmigera |
title_sort | computational metabolomics tools reveal subarmigerides, unprecedented linear peptides from the marine sponge holobiont callyspongia subarmigera |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696656/ https://www.ncbi.nlm.nih.gov/pubmed/36354996 http://dx.doi.org/10.3390/md20110673 |
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