Cargando…

Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats

Herein, zinc oxide nanoparticles (ZnO NPs) were greenly synthesized from Tridax procumbens aqueous leaf extract (TPE) and characterized physically (e.g., Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM)) and biologically (test of their anti-diabetic activity). An...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahmed, Syed S., Alqahtani, Ali M., Alqahtani, Taha, Alamri, Ali H., Menaa, Farid, Mani, Rupesh Kumar, D. R., Bharathi, Kavitha, Kunchu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696738/
https://www.ncbi.nlm.nih.gov/pubmed/36431686
http://dx.doi.org/10.3390/ma15228202
_version_ 1784838385337106432
author Ahmed, Syed S.
Alqahtani, Ali M.
Alqahtani, Taha
Alamri, Ali H.
Menaa, Farid
Mani, Rupesh Kumar
D. R., Bharathi
Kavitha, Kunchu
author_facet Ahmed, Syed S.
Alqahtani, Ali M.
Alqahtani, Taha
Alamri, Ali H.
Menaa, Farid
Mani, Rupesh Kumar
D. R., Bharathi
Kavitha, Kunchu
author_sort Ahmed, Syed S.
collection PubMed
description Herein, zinc oxide nanoparticles (ZnO NPs) were greenly synthesized from Tridax procumbens aqueous leaf extract (TPE) and characterized physically (e.g., Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM)) and biologically (test of their anti-diabetic activity). Anti-diabetic activities of TPE and TPE-derived ZnO NPs have been carried out in a streptozotocin (STZ)—induced diabetic rat model. Diabetes mellitus (DM) was induced with a single intraperitoneal dosage of the glucose analogue STZ (55 mg/Kg) known to be particularly toxic to pancreatic insulin-producing beta-cells. TPE and TPE-derived ZnO NPs were administered orally, once every day for 21 days in diabetic rats, at 100 and 200 mg/Kg, respectively. The standard antidiabetic medication, glibenclamide, was used as a control at a dose of 10 mg/Kg. Various parameters were investigated, including bodyweight (bw) variations, glycemia, lipidaemia, glycated hemoglobin (HbA1c), and histopathological alterations in the rat’s liver and pancreas. The TPE-mediated NPs were small, spherical, stable, and uniform. Compared to TPE and, to a lesser extent, glibenclamide, TPE-derived ZnO NPs lowered blood glucose levels considerably (p < 0.05) and in a dose-dependent manner while preventing body weight loss. Further, positive benefits for both the lipid profile and glycated hemoglobin were also noticed with TPE-derived ZnO NPs. The histopathological assessment revealed that synthesized TPE-derived ZnO NPs are safe, non-toxic, and biocompatible. At 200 mg/Kg/day, TPE-derived ZnO NPs had a more substantial hypoglycemic response than at 100 mg/Kg/day. Thus, in this first reported experimental setting, ZnO NPs biosynthesized from the leaf extract of Tridax procumbens exert more potent anti-diabetic activity than TPE and glibenclamide. We conclude that such a greenly prepared nanomaterial may be a promising alternative or complementary (adjuvant) therapy, at least to the current Indian’s traditional medicine system. Translational findings are prompted in human populations to determine the efficacy of these NPs.
format Online
Article
Text
id pubmed-9696738
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96967382022-11-26 Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats Ahmed, Syed S. Alqahtani, Ali M. Alqahtani, Taha Alamri, Ali H. Menaa, Farid Mani, Rupesh Kumar D. R., Bharathi Kavitha, Kunchu Materials (Basel) Article Herein, zinc oxide nanoparticles (ZnO NPs) were greenly synthesized from Tridax procumbens aqueous leaf extract (TPE) and characterized physically (e.g., Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM)) and biologically (test of their anti-diabetic activity). Anti-diabetic activities of TPE and TPE-derived ZnO NPs have been carried out in a streptozotocin (STZ)—induced diabetic rat model. Diabetes mellitus (DM) was induced with a single intraperitoneal dosage of the glucose analogue STZ (55 mg/Kg) known to be particularly toxic to pancreatic insulin-producing beta-cells. TPE and TPE-derived ZnO NPs were administered orally, once every day for 21 days in diabetic rats, at 100 and 200 mg/Kg, respectively. The standard antidiabetic medication, glibenclamide, was used as a control at a dose of 10 mg/Kg. Various parameters were investigated, including bodyweight (bw) variations, glycemia, lipidaemia, glycated hemoglobin (HbA1c), and histopathological alterations in the rat’s liver and pancreas. The TPE-mediated NPs were small, spherical, stable, and uniform. Compared to TPE and, to a lesser extent, glibenclamide, TPE-derived ZnO NPs lowered blood glucose levels considerably (p < 0.05) and in a dose-dependent manner while preventing body weight loss. Further, positive benefits for both the lipid profile and glycated hemoglobin were also noticed with TPE-derived ZnO NPs. The histopathological assessment revealed that synthesized TPE-derived ZnO NPs are safe, non-toxic, and biocompatible. At 200 mg/Kg/day, TPE-derived ZnO NPs had a more substantial hypoglycemic response than at 100 mg/Kg/day. Thus, in this first reported experimental setting, ZnO NPs biosynthesized from the leaf extract of Tridax procumbens exert more potent anti-diabetic activity than TPE and glibenclamide. We conclude that such a greenly prepared nanomaterial may be a promising alternative or complementary (adjuvant) therapy, at least to the current Indian’s traditional medicine system. Translational findings are prompted in human populations to determine the efficacy of these NPs. MDPI 2022-11-18 /pmc/articles/PMC9696738/ /pubmed/36431686 http://dx.doi.org/10.3390/ma15228202 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahmed, Syed S.
Alqahtani, Ali M.
Alqahtani, Taha
Alamri, Ali H.
Menaa, Farid
Mani, Rupesh Kumar
D. R., Bharathi
Kavitha, Kunchu
Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats
title Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats
title_full Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats
title_fullStr Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats
title_full_unstemmed Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats
title_short Green Synthesis, Characterizations of Zinc Oxide Nanoparticles from Aqueous Leaf Extract of Tridax procumbens Linn. and Assessment of their Anti-Hyperglycemic Activity in Streptozoticin-Induced Diabetic Rats
title_sort green synthesis, characterizations of zinc oxide nanoparticles from aqueous leaf extract of tridax procumbens linn. and assessment of their anti-hyperglycemic activity in streptozoticin-induced diabetic rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696738/
https://www.ncbi.nlm.nih.gov/pubmed/36431686
http://dx.doi.org/10.3390/ma15228202
work_keys_str_mv AT ahmedsyeds greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats
AT alqahtanialim greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats
AT alqahtanitaha greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats
AT alamrialih greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats
AT menaafarid greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats
AT manirupeshkumar greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats
AT drbharathi greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats
AT kavithakunchu greensynthesischaracterizationsofzincoxidenanoparticlesfromaqueousleafextractoftridaxprocumbenslinnandassessmentoftheirantihyperglycemicactivityinstreptozoticininduceddiabeticrats