Cargando…

Modular Site-Specific Conjugation of Nanobodies Using Two Co-Associating Tags

The homogeneous labeling of antibodies and their fragments is a critical step for the generation of robust probes used in immuno-detection applications. To date, numerous chemical, genetic and peptide-based site-specific coupling methods have been developed. Among these methods, co-assembling peptid...

Descripción completa

Detalles Bibliográficos
Autores principales:	Moeglin, Eric, Barret, Lina, Chatton, Bruno, Donzeau, Mariel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696751/ https://www.ncbi.nlm.nih.gov/pubmed/36430882 http://dx.doi.org/10.3390/ijms232214405

Ejemplares similares

The Prodigious Potential of mRNA Electrotransfer as a Substitute to Conventional DNA-Based Transient Transfection
por: Juncker, Théo, et al.
Publicado: (2023)

Gold labelling of a green fluorescent protein (GFP)-tag inside cells using recombinant nanobodies conjugated to 2.4 nm thiolate-coated gold nanoparticles
por: Groysbeck, Nadja, et al.
Publicado: (2021)

Site-specific nanobody-oligonucleotide conjugation for super-resolution imaging
por: Teodori, Laura, et al.
Publicado: (2022)

Sensitive Protein Detection Using Site-Specifically Oligonucleotide-Conjugated Nanobodies
por: Al-Amin, Rasel A., et al.
Publicado: (2022)

A Cytoplasmic Negative Regulator Isoform of ATF7 Impairs ATF7 and ATF2 Phosphorylation and Transcriptional Activity
por: Diring, Jessica, et al.
Publicado: (2011)

Nanobody Conjugates for Targeted Cancer Therapy and Imaging
por: Kang, Wei, et al.
Publicado: (2021)

Tyrosinase-Mediated Synthesis of Nanobody–Cell Conjugates
por: Maza, Johnathan C., et al.
Publicado: (2022)

A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
por: Virant, David, et al.
Publicado: (2018)

Fiber manipulation and post-assembly nanobody conjugation for adenoviral vector retargeting through SpyTag-SpyCatcher protein ligation
por: Kadkhodazadeh, Maryam, et al.
Publicado: (2022)

TOOLBOX: Strep-Tagged nano-assemblies of antibody-drug-conjugates (ADC) for modular and conditional cancer drugging
por: Tremante, Elisa, et al.
Publicado: (2021)

An efficient method for the site-specific (99m)Tc labeling of nanobody
por: Luo, Qi, et al.
Publicado: (2021)

A Modular Vaccine Development Platform Based on Sortase-Mediated Site-Specific Tagging of Antigens onto Virus-Like Particles
por: Tang, Shubing, et al.
Publicado: (2016)

Penetration of Nanobody-Dextran Polymer Conjugates through Tumor Spheroids
por: Bitsch, Peter, et al.
Publicado: (2023)

Site-specific C-terminal dinitrophenylation to reconstitute the antibody Fc functions for nanobodies
por: Hong, Haofei, et al.
Publicado: (2019)

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
por: Götzke, Hansjörg, et al.
Publicado: (2019)

Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins
por: Schneider, Falk, et al.
Publicado: (2020)

Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies
por: Xu, Jun, et al.
Publicado: (2022)

Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag
por: Bartosovic, Marek, et al.
Publicado: (2022)

Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots
por: Ramos-Gomes, Fernanda, et al.
Publicado: (2018)

An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
por: Cai, Hongmin, et al.
Publicado: (2020)

A Light‐Activatable Photocaged Variant of the Ultra‐High Affinity ALFA‐Tag Nanobody
por: Jedlitzke, Benedikt, et al.
Publicado: (2022)

mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells
por: Hatano, Tomoyuki, et al.
Publicado: (2022)

Small Quantum Dots Conjugated to Nanobodies as Immunofluorescence Probes for Nanometric Microscopy
por: Wang, Yong, et al.
Publicado: (2014)

Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses
por: Modi, Souvik, et al.
Publicado: (2018)

Covalent conjugation of extracellular vesicles with peptides and nanobodies for targeted therapeutic delivery
por: Pham, Tin Chanh, et al.
Publicado: (2021)

Selecting Nanobodies Specific for the Epidermal Growth Factor from a Synthetic Nanobody Library
por: Serrano-Rivero, Yunier, et al.
Publicado: (2023)

Nanobody Based Dual Specific CARs
por: De Munter, Stijn, et al.
Publicado: (2018)

Rational design of a DNA sequence-specific modular protein tag by tuning the alkylation kinetics
por: Nguyen, Thang Minh, et al.
Publicado: (2019)

Preventing SARS-CoV-2 Infection Using Anti-spike Nanobody-IFN-β Conjugated Exosomes
por: Lyu, Xiabing, et al.
Publicado: (2022)

Oriented Immobilization and Quantitative Analysis Simultaneously Realized in Sandwich Immunoassay via His-Tagged Nanobody
por: Xu, Li, et al.
Publicado: (2019)

Tagging the proteasome active site β5 causes tag specific phenotypes in yeast
por: Waite, Kenrick A., et al.
Publicado: (2020)

Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated Nanobodies
por: Soave, Mark, et al.
Publicado: (2020)

M-TAG: A modular teaching-aid for Geant4
por: Carroll, Liam, et al.
Publicado: (2023)

CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
por: Schmid-Burgk, Jonathan L., et al.
Publicado: (2016)

Site-specific conjugation of native antibody
por: Sadiki, Amissi, et al.
Publicado: (2020)

A Competitive Panning Method Reveals an Anti-SARS-CoV-2 Nanobody Specific for an RBD-ACE2 Binding Site
por: He, Siqi, et al.
Publicado: (2023)

Notes on modular conjugations of von Neumann factors
por: Leitz-Martini, M, et al.
Publicado: (1999)

Modular Synthetic Approach to Carboranyl‒Biomolecules Conjugates
por: Kellert, Martin, et al.
Publicado: (2021)

Evolution of nanobodies specific for BCL11A
por: Yin, Maolu, et al.
Publicado: (2023)

Generation of Synthetic Acinetobacter baumannii-Specific Nanobodies
por: Knauf, Gregory A., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ejch09gv733lilfo4d5lrneghn