Cargando…

MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms

Quorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as N-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on the heterogeneity o...

Descripción completa

Detalles Bibliográficos
Autores principales: Pitchapa, Rattanaburi, Dissook, Sivamoke, Putri, Sastia Prama, Fukusaki, Eiichiro, Shimma, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697013/
https://www.ncbi.nlm.nih.gov/pubmed/36422288
http://dx.doi.org/10.3390/metabo12111148
_version_ 1784838453601501184
author Pitchapa, Rattanaburi
Dissook, Sivamoke
Putri, Sastia Prama
Fukusaki, Eiichiro
Shimma, Shuichi
author_facet Pitchapa, Rattanaburi
Dissook, Sivamoke
Putri, Sastia Prama
Fukusaki, Eiichiro
Shimma, Shuichi
author_sort Pitchapa, Rattanaburi
collection PubMed
description Quorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as N-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on the heterogeneity of QS metabolites in biofilms remains largely unknown. Here, we describe the utilization of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to follow the production of specific metabolites, including QS metabolites, during Pseudomonas putida biofilm development. To do so, a method to grow an agar-based biofilm was first established, and MALDI-MSI was used to detect and visualize the distribution of QS metabolites in biofilms at different cultivation times. This study demonstrated that N-acyl-homoserine lactones are homogeneously produced in the early stages of P. putida biofilm formation. In contrast, the spatial distribution of quinolones and pyochelin correlated with the swarming motility of P. putida in mature biofilms. These two metabolites are involved in the production of extracellular polymeric substances and iron chelators. Our study thus contributes to establishing the specific temporal regulation and spatial distribution of N-acyl-homoserine lactone-related metabolites and quinolone and pyochelin in P. putida biofilms.
format Online
Article
Text
id pubmed-9697013
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96970132022-11-26 MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms Pitchapa, Rattanaburi Dissook, Sivamoke Putri, Sastia Prama Fukusaki, Eiichiro Shimma, Shuichi Metabolites Article Quorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as N-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on the heterogeneity of QS metabolites in biofilms remains largely unknown. Here, we describe the utilization of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to follow the production of specific metabolites, including QS metabolites, during Pseudomonas putida biofilm development. To do so, a method to grow an agar-based biofilm was first established, and MALDI-MSI was used to detect and visualize the distribution of QS metabolites in biofilms at different cultivation times. This study demonstrated that N-acyl-homoserine lactones are homogeneously produced in the early stages of P. putida biofilm formation. In contrast, the spatial distribution of quinolones and pyochelin correlated with the swarming motility of P. putida in mature biofilms. These two metabolites are involved in the production of extracellular polymeric substances and iron chelators. Our study thus contributes to establishing the specific temporal regulation and spatial distribution of N-acyl-homoserine lactone-related metabolites and quinolone and pyochelin in P. putida biofilms. MDPI 2022-11-21 /pmc/articles/PMC9697013/ /pubmed/36422288 http://dx.doi.org/10.3390/metabo12111148 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pitchapa, Rattanaburi
Dissook, Sivamoke
Putri, Sastia Prama
Fukusaki, Eiichiro
Shimma, Shuichi
MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms
title MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms
title_full MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms
title_fullStr MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms
title_full_unstemmed MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms
title_short MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms
title_sort maldi mass spectrometry imaging reveals the existence of an n-acyl-homoserine lactone quorum sensing system in pseudomonas putida biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697013/
https://www.ncbi.nlm.nih.gov/pubmed/36422288
http://dx.doi.org/10.3390/metabo12111148
work_keys_str_mv AT pitchaparattanaburi maldimassspectrometryimagingrevealstheexistenceofannacylhomoserinelactonequorumsensingsysteminpseudomonasputidabiofilms
AT dissooksivamoke maldimassspectrometryimagingrevealstheexistenceofannacylhomoserinelactonequorumsensingsysteminpseudomonasputidabiofilms
AT putrisastiaprama maldimassspectrometryimagingrevealstheexistenceofannacylhomoserinelactonequorumsensingsysteminpseudomonasputidabiofilms
AT fukusakieiichiro maldimassspectrometryimagingrevealstheexistenceofannacylhomoserinelactonequorumsensingsysteminpseudomonasputidabiofilms
AT shimmashuichi maldimassspectrometryimagingrevealstheexistenceofannacylhomoserinelactonequorumsensingsysteminpseudomonasputidabiofilms