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Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease
BACKGROUND: Alzheimer’s disease (AD) research has relied on mouse models overexpressing human mutant A βPP; however, newer generation knock-in models allow for physiological expression of amyloid-β protein precursor (AβPP) containing familial AD mutations where murine AβPP is edited with a humanized...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697055/ https://www.ncbi.nlm.nih.gov/pubmed/36155507 http://dx.doi.org/10.3233/JAD-220383 |
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author | Dentoni, Giacomo Naia, Luana Portal, Benjamin Leal, Nuno Santos Nilsson, Per Lindskog, Maria Ankarcrona, Maria |
author_facet | Dentoni, Giacomo Naia, Luana Portal, Benjamin Leal, Nuno Santos Nilsson, Per Lindskog, Maria Ankarcrona, Maria |
author_sort | Dentoni, Giacomo |
collection | PubMed |
description | BACKGROUND: Alzheimer’s disease (AD) research has relied on mouse models overexpressing human mutant A βPP; however, newer generation knock-in models allow for physiological expression of amyloid-β protein precursor (AβPP) containing familial AD mutations where murine AβPP is edited with a humanized amyloid-β (Aβ) sequence. The App(NL-F) mouse model has shown substantial similarities to AD brains developing late onset cognitive impairment. OBJECTIVE: In this study, we aimed to characterize mature primary cortical neurons derived from homozygous App(NL-F) embryos, especially to identify early mitochondrial alterations in this model. METHODS: Primary cultures of App(NL-F) neurons kept in culture for 12–15 days were used to measure Aβ levels, secretase activity, mitochondrial functions, mitochondrial-ER contacts, synaptic function, and cell death. RESULTS: We detected higher levels of Aβ(42) released from App(NL-F) neurons as compared to wild-type neurons. App(NL-F) neurons, also displayed an increased Aβ(42)/Aβ(40) ratio, similar to adult App(NL-F) mouse brain. Interestingly, we found an upregulation in mitochondrial oxygen consumption with concomitant downregulation in glycolytic reserve. Furthermore, App(NL-F) neurons were more susceptible to cell death triggered by mitochondrial electron transport chain inhibition. Juxtaposition between ER and mitochondria was found to be substantially upregulated, which may account for upregulated mitochondrial-derived ATP production. However, anterograde mitochondrial movement was severely impaired in this model along with loss in synaptic vesicle protein and impairment in pre- and post-synaptic function. CONCLUSION: We show that widespread mitochondrial alterations can be detected in App(NL-F) neurons in vitro, where amyloid plaque deposition does not occur, suggesting soluble and oligomeric Aβ-species being responsible for these alterations. |
format | Online Article Text |
id | pubmed-9697055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-96970552022-12-08 Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease Dentoni, Giacomo Naia, Luana Portal, Benjamin Leal, Nuno Santos Nilsson, Per Lindskog, Maria Ankarcrona, Maria J Alzheimers Dis Research Article BACKGROUND: Alzheimer’s disease (AD) research has relied on mouse models overexpressing human mutant A βPP; however, newer generation knock-in models allow for physiological expression of amyloid-β protein precursor (AβPP) containing familial AD mutations where murine AβPP is edited with a humanized amyloid-β (Aβ) sequence. The App(NL-F) mouse model has shown substantial similarities to AD brains developing late onset cognitive impairment. OBJECTIVE: In this study, we aimed to characterize mature primary cortical neurons derived from homozygous App(NL-F) embryos, especially to identify early mitochondrial alterations in this model. METHODS: Primary cultures of App(NL-F) neurons kept in culture for 12–15 days were used to measure Aβ levels, secretase activity, mitochondrial functions, mitochondrial-ER contacts, synaptic function, and cell death. RESULTS: We detected higher levels of Aβ(42) released from App(NL-F) neurons as compared to wild-type neurons. App(NL-F) neurons, also displayed an increased Aβ(42)/Aβ(40) ratio, similar to adult App(NL-F) mouse brain. Interestingly, we found an upregulation in mitochondrial oxygen consumption with concomitant downregulation in glycolytic reserve. Furthermore, App(NL-F) neurons were more susceptible to cell death triggered by mitochondrial electron transport chain inhibition. Juxtaposition between ER and mitochondria was found to be substantially upregulated, which may account for upregulated mitochondrial-derived ATP production. However, anterograde mitochondrial movement was severely impaired in this model along with loss in synaptic vesicle protein and impairment in pre- and post-synaptic function. CONCLUSION: We show that widespread mitochondrial alterations can be detected in App(NL-F) neurons in vitro, where amyloid plaque deposition does not occur, suggesting soluble and oligomeric Aβ-species being responsible for these alterations. IOS Press 2022-11-08 /pmc/articles/PMC9697055/ /pubmed/36155507 http://dx.doi.org/10.3233/JAD-220383 Text en © 2022 – The authors. Published by IOS Press https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dentoni, Giacomo Naia, Luana Portal, Benjamin Leal, Nuno Santos Nilsson, Per Lindskog, Maria Ankarcrona, Maria Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease |
title | Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease |
title_full | Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease |
title_fullStr | Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease |
title_full_unstemmed | Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease |
title_short | Mitochondrial Alterations in Neurons Derived from the Murine App(NL-F) Knock-In Model of Alzheimer’s Disease |
title_sort | mitochondrial alterations in neurons derived from the murine app(nl-f) knock-in model of alzheimer’s disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697055/ https://www.ncbi.nlm.nih.gov/pubmed/36155507 http://dx.doi.org/10.3233/JAD-220383 |
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