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Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells
Late-onset hypogonadism, a male age-related syndrome characterized by a decline in testosterone production in the testes, is commonly treated with testosterone replacement therapy, which has adverse side effects. Therefore, an alternative treatment is highly sought. Supplementation of a high dosage...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697070/ https://www.ncbi.nlm.nih.gov/pubmed/36432448 http://dx.doi.org/10.3390/nu14224761 |
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author | Shiozawa, Kota Maeda, Misato Ho, Hsin-Jung Katsurai, Tomoko Howlader, Md. Zakir Hossain Horiuchi, Kimiko Sugita, Yumi Ohsaki, Yusuke Agista, Afifah Zahra Goto, Tomoko Komai, Michio Shirakawa, Hitoshi |
author_facet | Shiozawa, Kota Maeda, Misato Ho, Hsin-Jung Katsurai, Tomoko Howlader, Md. Zakir Hossain Horiuchi, Kimiko Sugita, Yumi Ohsaki, Yusuke Agista, Afifah Zahra Goto, Tomoko Komai, Michio Shirakawa, Hitoshi |
author_sort | Shiozawa, Kota |
collection | PubMed |
description | Late-onset hypogonadism, a male age-related syndrome characterized by a decline in testosterone production in the testes, is commonly treated with testosterone replacement therapy, which has adverse side effects. Therefore, an alternative treatment is highly sought. Supplementation of a high dosage of biotin, a water-soluble vitamin that functions as a coenzyme for carboxylases involved in carbohydrate, lipid, and amino acid metabolism, has been shown to influence testis functions. However, the involvement of biotin in testis steroidogenesis has not been well clarified. In this study, we examined the effect of biotin on testosterone levels in mice and testis-derived cells. In mice, intraperitoneal treatment with biotin (1.5 mg/kg body weight) enhanced testosterone levels in the serum and testes, without elevating serum levels of pituitary luteinizing hormone. To investigate the mechanism in which biotin increased the testosterone level, mice testis-derived I-10 cells were used. The cells treated with biotin increased testosterone production in a dose- and time-dependent manner. Biotin treatment elevated intracellular cyclic adenosine monophosphate levels via adenylate cyclase activation, followed by the activation of protein kinase A and testosterone production. These results suggest that biotin may have the potential to improve age-related male syndromes associated with declining testosterone production. |
format | Online Article Text |
id | pubmed-9697070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96970702022-11-26 Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells Shiozawa, Kota Maeda, Misato Ho, Hsin-Jung Katsurai, Tomoko Howlader, Md. Zakir Hossain Horiuchi, Kimiko Sugita, Yumi Ohsaki, Yusuke Agista, Afifah Zahra Goto, Tomoko Komai, Michio Shirakawa, Hitoshi Nutrients Article Late-onset hypogonadism, a male age-related syndrome characterized by a decline in testosterone production in the testes, is commonly treated with testosterone replacement therapy, which has adverse side effects. Therefore, an alternative treatment is highly sought. Supplementation of a high dosage of biotin, a water-soluble vitamin that functions as a coenzyme for carboxylases involved in carbohydrate, lipid, and amino acid metabolism, has been shown to influence testis functions. However, the involvement of biotin in testis steroidogenesis has not been well clarified. In this study, we examined the effect of biotin on testosterone levels in mice and testis-derived cells. In mice, intraperitoneal treatment with biotin (1.5 mg/kg body weight) enhanced testosterone levels in the serum and testes, without elevating serum levels of pituitary luteinizing hormone. To investigate the mechanism in which biotin increased the testosterone level, mice testis-derived I-10 cells were used. The cells treated with biotin increased testosterone production in a dose- and time-dependent manner. Biotin treatment elevated intracellular cyclic adenosine monophosphate levels via adenylate cyclase activation, followed by the activation of protein kinase A and testosterone production. These results suggest that biotin may have the potential to improve age-related male syndromes associated with declining testosterone production. MDPI 2022-11-10 /pmc/articles/PMC9697070/ /pubmed/36432448 http://dx.doi.org/10.3390/nu14224761 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shiozawa, Kota Maeda, Misato Ho, Hsin-Jung Katsurai, Tomoko Howlader, Md. Zakir Hossain Horiuchi, Kimiko Sugita, Yumi Ohsaki, Yusuke Agista, Afifah Zahra Goto, Tomoko Komai, Michio Shirakawa, Hitoshi Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells |
title | Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells |
title_full | Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells |
title_fullStr | Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells |
title_full_unstemmed | Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells |
title_short | Biotin Enhances Testosterone Production in Mice and Their Testis-Derived Cells |
title_sort | biotin enhances testosterone production in mice and their testis-derived cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697070/ https://www.ncbi.nlm.nih.gov/pubmed/36432448 http://dx.doi.org/10.3390/nu14224761 |
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