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Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018

It is important i to monitor the transmission and antimicrobial resistance of Neisseria gonorrhoeae (NG). Current surveillance relies on culturing, which frequently fails. Previously, a culture-independent genotyping method was developed based on NG multi-antigen sequence typing (NG-MAST). To determ...

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Autores principales: Slaats, Michiel H. C., van der Veer, Brian M. J. W., van Alphen, Lieke B., Hoebe, Christian J. P. A., Dukers-Muijrers, Nicole H. T. M., Wolffs, Petra F. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697099/
https://www.ncbi.nlm.nih.gov/pubmed/36422595
http://dx.doi.org/10.3390/pathogens11111344
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author Slaats, Michiel H. C.
van der Veer, Brian M. J. W.
van Alphen, Lieke B.
Hoebe, Christian J. P. A.
Dukers-Muijrers, Nicole H. T. M.
Wolffs, Petra F. G.
author_facet Slaats, Michiel H. C.
van der Veer, Brian M. J. W.
van Alphen, Lieke B.
Hoebe, Christian J. P. A.
Dukers-Muijrers, Nicole H. T. M.
Wolffs, Petra F. G.
author_sort Slaats, Michiel H. C.
collection PubMed
description It is important i to monitor the transmission and antimicrobial resistance of Neisseria gonorrhoeae (NG). Current surveillance relies on culturing, which frequently fails. Previously, a culture-independent genotyping method was developed based on NG multi-antigen sequence typing (NG-MAST). To determine whether crucial sequence types (STs) are missed during culture-dependent surveillance, NG-positive NAAT samples were genotyped, and the results of the culture-positive and culture-negative samples were compared. In total, 196 NG-positive NAAT samples, from January 2017 until August 2018, which were also routinely cultured, were retrospectively included. Genotyping was successful in 152 NAAT samples (77.0%), 33 NAAT samples failed, and 11 NAAT samples showed possible mixed strain infections. Oropharyngeal samples (n = 16) showed the largest increase in typing rate from 6.3% (1/16) success in culture-dependent genotyping to 81.3% (13/16) in culture-independent genotyping. Nine genogroups (n ≥ 5 samples) were found; all included both culture-positive and culture-negative NG. However, culture-independent surveillance revealed 14 additional STs in the culture-negative samples. Overall, culture-dependent surveillance could detect all genogroups, indicating that major trends could be identified with culture-dependent surveillance. However, culture-independent surveillance provides more STs, mixed infections and more oropharyngeal samples, giving a more detailed view and could result in an earlier detection of outbreaks and transmission.
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spelling pubmed-96970992022-11-26 Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018 Slaats, Michiel H. C. van der Veer, Brian M. J. W. van Alphen, Lieke B. Hoebe, Christian J. P. A. Dukers-Muijrers, Nicole H. T. M. Wolffs, Petra F. G. Pathogens Article It is important i to monitor the transmission and antimicrobial resistance of Neisseria gonorrhoeae (NG). Current surveillance relies on culturing, which frequently fails. Previously, a culture-independent genotyping method was developed based on NG multi-antigen sequence typing (NG-MAST). To determine whether crucial sequence types (STs) are missed during culture-dependent surveillance, NG-positive NAAT samples were genotyped, and the results of the culture-positive and culture-negative samples were compared. In total, 196 NG-positive NAAT samples, from January 2017 until August 2018, which were also routinely cultured, were retrospectively included. Genotyping was successful in 152 NAAT samples (77.0%), 33 NAAT samples failed, and 11 NAAT samples showed possible mixed strain infections. Oropharyngeal samples (n = 16) showed the largest increase in typing rate from 6.3% (1/16) success in culture-dependent genotyping to 81.3% (13/16) in culture-independent genotyping. Nine genogroups (n ≥ 5 samples) were found; all included both culture-positive and culture-negative NG. However, culture-independent surveillance revealed 14 additional STs in the culture-negative samples. Overall, culture-dependent surveillance could detect all genogroups, indicating that major trends could be identified with culture-dependent surveillance. However, culture-independent surveillance provides more STs, mixed infections and more oropharyngeal samples, giving a more detailed view and could result in an earlier detection of outbreaks and transmission. MDPI 2022-11-14 /pmc/articles/PMC9697099/ /pubmed/36422595 http://dx.doi.org/10.3390/pathogens11111344 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Slaats, Michiel H. C.
van der Veer, Brian M. J. W.
van Alphen, Lieke B.
Hoebe, Christian J. P. A.
Dukers-Muijrers, Nicole H. T. M.
Wolffs, Petra F. G.
Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018
title Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018
title_full Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018
title_fullStr Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018
title_full_unstemmed Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018
title_short Culture-Independent Genotyping Improves Surveillance of Neisseria gonorrhoeae, Especially in Oropharyngeal Samples, the Netherlands, 2017 to 2018
title_sort culture-independent genotyping improves surveillance of neisseria gonorrhoeae, especially in oropharyngeal samples, the netherlands, 2017 to 2018
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697099/
https://www.ncbi.nlm.nih.gov/pubmed/36422595
http://dx.doi.org/10.3390/pathogens11111344
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