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The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages
STING is a transmembrane ER resident protein that was initially described as a regulator of innate immune response triggered by viral DNA and later found to be involved in a broader range of immune processes. Here, we assessed its role in the antigen presentation by generating a STING KO macrophage...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697192/ https://www.ncbi.nlm.nih.gov/pubmed/36430709 http://dx.doi.org/10.3390/ijms232214232 |
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author | Caiazza, Carmen Brusco, Teresa D’Alessio, Federica D’Agostino, Massimo Avagliano, Angelica Arcucci, Alessandro Ambrosino, Concetta Fiume, Giuseppe Mallardo, Massimo |
author_facet | Caiazza, Carmen Brusco, Teresa D’Alessio, Federica D’Agostino, Massimo Avagliano, Angelica Arcucci, Alessandro Ambrosino, Concetta Fiume, Giuseppe Mallardo, Massimo |
author_sort | Caiazza, Carmen |
collection | PubMed |
description | STING is a transmembrane ER resident protein that was initially described as a regulator of innate immune response triggered by viral DNA and later found to be involved in a broader range of immune processes. Here, we assessed its role in the antigen presentation by generating a STING KO macrophage cell line. In the absence of STING, we observed an impaired OVA-derived SIINFEKL peptide presentation together with a decreased level of MHC-I complex on the plasma membrane, likely due to a decreased mRNA expression of β2 m light chain as no relevant alterations of the peptide-loading complex (TAPs) were found. Moreover, JAK-STAT signaling resulted in impaired STING KO cells following OVA and LPS treatments, suggesting a dampened activation of immune response. Our data revealed a new molecular role of STING in immune mechanisms that could elucidate its role in the pathogenesis of autoimmune disorders and cancer. |
format | Online Article Text |
id | pubmed-9697192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96971922022-11-26 The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages Caiazza, Carmen Brusco, Teresa D’Alessio, Federica D’Agostino, Massimo Avagliano, Angelica Arcucci, Alessandro Ambrosino, Concetta Fiume, Giuseppe Mallardo, Massimo Int J Mol Sci Article STING is a transmembrane ER resident protein that was initially described as a regulator of innate immune response triggered by viral DNA and later found to be involved in a broader range of immune processes. Here, we assessed its role in the antigen presentation by generating a STING KO macrophage cell line. In the absence of STING, we observed an impaired OVA-derived SIINFEKL peptide presentation together with a decreased level of MHC-I complex on the plasma membrane, likely due to a decreased mRNA expression of β2 m light chain as no relevant alterations of the peptide-loading complex (TAPs) were found. Moreover, JAK-STAT signaling resulted in impaired STING KO cells following OVA and LPS treatments, suggesting a dampened activation of immune response. Our data revealed a new molecular role of STING in immune mechanisms that could elucidate its role in the pathogenesis of autoimmune disorders and cancer. MDPI 2022-11-17 /pmc/articles/PMC9697192/ /pubmed/36430709 http://dx.doi.org/10.3390/ijms232214232 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Caiazza, Carmen Brusco, Teresa D’Alessio, Federica D’Agostino, Massimo Avagliano, Angelica Arcucci, Alessandro Ambrosino, Concetta Fiume, Giuseppe Mallardo, Massimo The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages |
title | The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages |
title_full | The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages |
title_fullStr | The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages |
title_full_unstemmed | The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages |
title_short | The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages |
title_sort | lack of sting impairs the mhc-i dependent antigen presentation and jak/stat signaling in murine macrophages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697192/ https://www.ncbi.nlm.nih.gov/pubmed/36430709 http://dx.doi.org/10.3390/ijms232214232 |
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