Cargando…
Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination
We prospectively studied immunological response against SARS-CoV-2 after vaccination among healthcare workers without (group A) and with previous infection (group B). The analyses were collected at T0 (before the BNT162b2), T1 (before the second dose), T2 and T6 (1 and 6 months after the second dose...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697204/ https://www.ncbi.nlm.nih.gov/pubmed/36366293 http://dx.doi.org/10.3390/vaccines10111784 |
_version_ | 1784838502605651968 |
---|---|
author | Dentone, Chiara Fenoglio, Daniela Ponzano, Marta Cerchiaro, Matteo Altosole, Tiziana Franciotta, Diego Portunato, Federica Mikulska, Malgorzata Taramasso, Lucia Magnasco, Laura Uras, Chiara Magne, Federica Ferrera, Francesca Scavone, Graziana Signori, Alessio Vena, Antonio Visconti, Valeria Filaci, Gilberto Sette, Alessandro Grifoni, Alba Di Biagio, Antonio Bassetti, Matteo |
author_facet | Dentone, Chiara Fenoglio, Daniela Ponzano, Marta Cerchiaro, Matteo Altosole, Tiziana Franciotta, Diego Portunato, Federica Mikulska, Malgorzata Taramasso, Lucia Magnasco, Laura Uras, Chiara Magne, Federica Ferrera, Francesca Scavone, Graziana Signori, Alessio Vena, Antonio Visconti, Valeria Filaci, Gilberto Sette, Alessandro Grifoni, Alba Di Biagio, Antonio Bassetti, Matteo |
author_sort | Dentone, Chiara |
collection | PubMed |
description | We prospectively studied immunological response against SARS-CoV-2 after vaccination among healthcare workers without (group A) and with previous infection (group B). The analyses were collected at T0 (before the BNT162b2), T1 (before the second dose), T2 and T6 (1 and 6 months after the second dose). For cellular immune response, the activation-induced cell marker assay was performed with CD4 and CD8 Spike peptide megapools expressed as Stimulation Index. For humoral immune response, we determined antibodies to Spike-1 and nucleocapsid protein. The linear mixed model compared specific times to T0. The CD4+ Spike response overall rate of change was significant at T1 (p = 0.038) and at T2 (p < 0.001), while decreasing at T6. For CD8+ Spike reactivity, the interaction between the time and group was significant (p = 0.0265), and the p value for group comparison was significant at the baseline (p = 0.0030) with higher SI in previously infected subjects. Overall, the anti-S Abs significantly increased from T1 to T6 compared to T0. The group B at T6 retained high anti-S titer (p < 0.001). At T6, in both groups we found a persistent humoral response and a high CD4+ T cell response able to cross recognize SARS-COV-2 variants including epsilon, even if not a circulating virus at that time. |
format | Online Article Text |
id | pubmed-9697204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96972042022-11-26 Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination Dentone, Chiara Fenoglio, Daniela Ponzano, Marta Cerchiaro, Matteo Altosole, Tiziana Franciotta, Diego Portunato, Federica Mikulska, Malgorzata Taramasso, Lucia Magnasco, Laura Uras, Chiara Magne, Federica Ferrera, Francesca Scavone, Graziana Signori, Alessio Vena, Antonio Visconti, Valeria Filaci, Gilberto Sette, Alessandro Grifoni, Alba Di Biagio, Antonio Bassetti, Matteo Vaccines (Basel) Article We prospectively studied immunological response against SARS-CoV-2 after vaccination among healthcare workers without (group A) and with previous infection (group B). The analyses were collected at T0 (before the BNT162b2), T1 (before the second dose), T2 and T6 (1 and 6 months after the second dose). For cellular immune response, the activation-induced cell marker assay was performed with CD4 and CD8 Spike peptide megapools expressed as Stimulation Index. For humoral immune response, we determined antibodies to Spike-1 and nucleocapsid protein. The linear mixed model compared specific times to T0. The CD4+ Spike response overall rate of change was significant at T1 (p = 0.038) and at T2 (p < 0.001), while decreasing at T6. For CD8+ Spike reactivity, the interaction between the time and group was significant (p = 0.0265), and the p value for group comparison was significant at the baseline (p = 0.0030) with higher SI in previously infected subjects. Overall, the anti-S Abs significantly increased from T1 to T6 compared to T0. The group B at T6 retained high anti-S titer (p < 0.001). At T6, in both groups we found a persistent humoral response and a high CD4+ T cell response able to cross recognize SARS-COV-2 variants including epsilon, even if not a circulating virus at that time. MDPI 2022-10-24 /pmc/articles/PMC9697204/ /pubmed/36366293 http://dx.doi.org/10.3390/vaccines10111784 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dentone, Chiara Fenoglio, Daniela Ponzano, Marta Cerchiaro, Matteo Altosole, Tiziana Franciotta, Diego Portunato, Federica Mikulska, Malgorzata Taramasso, Lucia Magnasco, Laura Uras, Chiara Magne, Federica Ferrera, Francesca Scavone, Graziana Signori, Alessio Vena, Antonio Visconti, Valeria Filaci, Gilberto Sette, Alessandro Grifoni, Alba Di Biagio, Antonio Bassetti, Matteo Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination |
title | Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination |
title_full | Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination |
title_fullStr | Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination |
title_full_unstemmed | Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination |
title_short | Healthcare Worker Study Cohort to Determine the Level and Durability of Cellular and Humoral Immune Responses after Two Doses of SARS-CoV-2 Vaccination |
title_sort | healthcare worker study cohort to determine the level and durability of cellular and humoral immune responses after two doses of sars-cov-2 vaccination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697204/ https://www.ncbi.nlm.nih.gov/pubmed/36366293 http://dx.doi.org/10.3390/vaccines10111784 |
work_keys_str_mv | AT dentonechiara healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT fenogliodaniela healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT ponzanomarta healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT cerchiaromatteo healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT altosoletiziana healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT franciottadiego healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT portunatofederica healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT mikulskamalgorzata healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT taramassolucia healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT magnascolaura healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT uraschiara healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT magnefederica healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT ferrerafrancesca healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT scavonegraziana healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT signorialessio healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT venaantonio healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT viscontivaleria healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT filacigilberto healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT settealessandro healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT grifonialba healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT dibiagioantonio healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination AT bassettimatteo healthcareworkerstudycohorttodeterminethelevelanddurabilityofcellularandhumoralimmuneresponsesaftertwodosesofsarscov2vaccination |