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Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients

Human leucocyte antigens (HLAs) are highly polymorphic glycoproteins expressed at the surface of all nucleated cells. It is required for the SARS-CoV-2 peptide antigen presentation to immune cells for their effector response. However, polymorphism in HLA significantly impacts the binding of SARS-CoV...

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Autores principales: Prasad, Narayan, Yadav, Brijesh, Prakash, Swayam, Yadav, Deependra, Singh, Ankita, Gautam, Sonam, Bhadauria, Dharmendra, Kaul, Anupama, Patel, Manas Ranjan, Behera, Manas Ranjan, Kushwaha, Ravi Shankar, Yachha, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697220/
https://www.ncbi.nlm.nih.gov/pubmed/36366349
http://dx.doi.org/10.3390/vaccines10111840
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author Prasad, Narayan
Yadav, Brijesh
Prakash, Swayam
Yadav, Deependra
Singh, Ankita
Gautam, Sonam
Bhadauria, Dharmendra
Kaul, Anupama
Patel, Manas Ranjan
Behera, Manas Ranjan
Kushwaha, Ravi Shankar
Yachha, Monika
author_facet Prasad, Narayan
Yadav, Brijesh
Prakash, Swayam
Yadav, Deependra
Singh, Ankita
Gautam, Sonam
Bhadauria, Dharmendra
Kaul, Anupama
Patel, Manas Ranjan
Behera, Manas Ranjan
Kushwaha, Ravi Shankar
Yachha, Monika
author_sort Prasad, Narayan
collection PubMed
description Human leucocyte antigens (HLAs) are highly polymorphic glycoproteins expressed at the surface of all nucleated cells. It is required for the SARS-CoV-2 peptide antigen presentation to immune cells for their effector response. However, polymorphism in HLA significantly impacts the binding of SARS-CoV-2 antigenic peptide to the HLA pocket and regulates immune activation. In this study, 514 renal transplant recipients (RTRs) were recruited from the outpatient department and categorized either into symptomatic (n = 173) or asymptomatic groups (n = 341) based on Coronavirus disease-19 (COVID-19) symptoms. The anti-SARS-CoV-2 spike protein-specific IgG antibody titer was measured by chemiluminescent microparticle immune-assay methods in 310 RTRs. The HLA details of 514 patients were retrieved from the electronic medical records and analyzed retrospectively. We found that HLA antigen allele A*24 was significantly associated with asymptomatic infection in 22.78%, HLA C*02 in 4.51%, DRB1*12 in 10.85%, and HLA DQA1*02 in 27.74% of RTRs. Whereas HLA A*29 in 3.46%, A*33 in 26.01%, B*13 in 10.40%, DRB1*10 in 4.62%, DRB1*15 in 39.30%, DRB1*30 in 1.15%, and DQA1*60 in 3.57% of RTRs were associated with symptomatic infection. HLA DRB1*13 and DRB1*15 were associated with moderate to severe degrees of COVID-19 disease. The seroconversion rate in asymptomatic patients was 118/137 (86.13%), had a median titer of 647.80 au/mL, compared to symptomatic patients 148/173 (85.54%) with a median titer of 400.00 au/mL, which was not significant between the two groups (p = 0.88 and 0.13). In conclusion, HLA alleles A*24, C*02, DRB1*12, and DQA1*02 were significantly associated with asymptomatic infection, and A*29, A*33, B*13, DRB1*10, DRB*15, and DRB1*30 were significantly associated with symptomatic infection. HLA DRB1*13 and DRB1*15 were associated with moderate to severe degrees of COVID-19 disease.
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spelling pubmed-96972202022-11-26 Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients Prasad, Narayan Yadav, Brijesh Prakash, Swayam Yadav, Deependra Singh, Ankita Gautam, Sonam Bhadauria, Dharmendra Kaul, Anupama Patel, Manas Ranjan Behera, Manas Ranjan Kushwaha, Ravi Shankar Yachha, Monika Vaccines (Basel) Article Human leucocyte antigens (HLAs) are highly polymorphic glycoproteins expressed at the surface of all nucleated cells. It is required for the SARS-CoV-2 peptide antigen presentation to immune cells for their effector response. However, polymorphism in HLA significantly impacts the binding of SARS-CoV-2 antigenic peptide to the HLA pocket and regulates immune activation. In this study, 514 renal transplant recipients (RTRs) were recruited from the outpatient department and categorized either into symptomatic (n = 173) or asymptomatic groups (n = 341) based on Coronavirus disease-19 (COVID-19) symptoms. The anti-SARS-CoV-2 spike protein-specific IgG antibody titer was measured by chemiluminescent microparticle immune-assay methods in 310 RTRs. The HLA details of 514 patients were retrieved from the electronic medical records and analyzed retrospectively. We found that HLA antigen allele A*24 was significantly associated with asymptomatic infection in 22.78%, HLA C*02 in 4.51%, DRB1*12 in 10.85%, and HLA DQA1*02 in 27.74% of RTRs. Whereas HLA A*29 in 3.46%, A*33 in 26.01%, B*13 in 10.40%, DRB1*10 in 4.62%, DRB1*15 in 39.30%, DRB1*30 in 1.15%, and DQA1*60 in 3.57% of RTRs were associated with symptomatic infection. HLA DRB1*13 and DRB1*15 were associated with moderate to severe degrees of COVID-19 disease. The seroconversion rate in asymptomatic patients was 118/137 (86.13%), had a median titer of 647.80 au/mL, compared to symptomatic patients 148/173 (85.54%) with a median titer of 400.00 au/mL, which was not significant between the two groups (p = 0.88 and 0.13). In conclusion, HLA alleles A*24, C*02, DRB1*12, and DQA1*02 were significantly associated with asymptomatic infection, and A*29, A*33, B*13, DRB1*10, DRB*15, and DRB1*30 were significantly associated with symptomatic infection. HLA DRB1*13 and DRB1*15 were associated with moderate to severe degrees of COVID-19 disease. MDPI 2022-10-30 /pmc/articles/PMC9697220/ /pubmed/36366349 http://dx.doi.org/10.3390/vaccines10111840 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Prasad, Narayan
Yadav, Brijesh
Prakash, Swayam
Yadav, Deependra
Singh, Ankita
Gautam, Sonam
Bhadauria, Dharmendra
Kaul, Anupama
Patel, Manas Ranjan
Behera, Manas Ranjan
Kushwaha, Ravi Shankar
Yachha, Monika
Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients
title Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients
title_full Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients
title_fullStr Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients
title_full_unstemmed Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients
title_short Association of Human Leucocyte Antigen Polymorphism with Coronavirus Disease 19 in Renal Transplant Recipients
title_sort association of human leucocyte antigen polymorphism with coronavirus disease 19 in renal transplant recipients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697220/
https://www.ncbi.nlm.nih.gov/pubmed/36366349
http://dx.doi.org/10.3390/vaccines10111840
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