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Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge

Health-related concerns about cyanobacteria-laden sludge of drinking water treatment plants (DWTPs) have been raised in the past few years. Microscopic taxonomy, shotgun metagenomic sequencing, and microcystin (MC) measurement were applied to study the fate of cyanobacteria and cyanotoxins after con...

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Autores principales: Jalili, Farhad, Trigui, Hana, Maldonado, Juan Francisco Guerra, Dorner, Sarah, Zamyadi, Arash, Shapiro, B. Jesse, Terrat, Yves, Fortin, Nathalie, Sauvé, Sébastien, Prévost, Michèle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697381/
https://www.ncbi.nlm.nih.gov/pubmed/36355999
http://dx.doi.org/10.3390/toxins14110749
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author Jalili, Farhad
Trigui, Hana
Maldonado, Juan Francisco Guerra
Dorner, Sarah
Zamyadi, Arash
Shapiro, B. Jesse
Terrat, Yves
Fortin, Nathalie
Sauvé, Sébastien
Prévost, Michèle
author_facet Jalili, Farhad
Trigui, Hana
Maldonado, Juan Francisco Guerra
Dorner, Sarah
Zamyadi, Arash
Shapiro, B. Jesse
Terrat, Yves
Fortin, Nathalie
Sauvé, Sébastien
Prévost, Michèle
author_sort Jalili, Farhad
collection PubMed
description Health-related concerns about cyanobacteria-laden sludge of drinking water treatment plants (DWTPs) have been raised in the past few years. Microscopic taxonomy, shotgun metagenomic sequencing, and microcystin (MC) measurement were applied to study the fate of cyanobacteria and cyanotoxins after controlled sludge storage (stagnation) in the dark in a full-scale drinking water treatment plant within 7 to 38 days. For four out of eight dates, cyanobacterial cell growth was observed by total taxonomic cell counts during sludge stagnation. The highest observed cell growth was 96% after 16 days of stagnation. Cell growth was dominated by potential MC producers such as Microcystis, Aphanocapsa, Chroococcus, and Dolichospermum. Shotgun metagenomic sequencing unveiled that stagnation stress shifts the cyanobacterial communities from the stress-sensitive Nostocales (e.g., Dolichospermum) order towards less compromised orders and potential MC producers such as Chroococcales (e.g., Microcystis) and Synechococcales (e.g., Synechococcus). The relative increase of cyanotoxin producers presents a health challenge when the supernatant of the stored sludge is recycled to the head of the DWTP or discharged into the source. These findings emphasize the importance of a strategy to manage cyanobacteria-laden sludge and suggest practical approaches should be adopted to control health/environmental impacts of cyanobacteria and cyanotoxins in sludge.
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spelling pubmed-96973812022-11-26 Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge Jalili, Farhad Trigui, Hana Maldonado, Juan Francisco Guerra Dorner, Sarah Zamyadi, Arash Shapiro, B. Jesse Terrat, Yves Fortin, Nathalie Sauvé, Sébastien Prévost, Michèle Toxins (Basel) Article Health-related concerns about cyanobacteria-laden sludge of drinking water treatment plants (DWTPs) have been raised in the past few years. Microscopic taxonomy, shotgun metagenomic sequencing, and microcystin (MC) measurement were applied to study the fate of cyanobacteria and cyanotoxins after controlled sludge storage (stagnation) in the dark in a full-scale drinking water treatment plant within 7 to 38 days. For four out of eight dates, cyanobacterial cell growth was observed by total taxonomic cell counts during sludge stagnation. The highest observed cell growth was 96% after 16 days of stagnation. Cell growth was dominated by potential MC producers such as Microcystis, Aphanocapsa, Chroococcus, and Dolichospermum. Shotgun metagenomic sequencing unveiled that stagnation stress shifts the cyanobacterial communities from the stress-sensitive Nostocales (e.g., Dolichospermum) order towards less compromised orders and potential MC producers such as Chroococcales (e.g., Microcystis) and Synechococcales (e.g., Synechococcus). The relative increase of cyanotoxin producers presents a health challenge when the supernatant of the stored sludge is recycled to the head of the DWTP or discharged into the source. These findings emphasize the importance of a strategy to manage cyanobacteria-laden sludge and suggest practical approaches should be adopted to control health/environmental impacts of cyanobacteria and cyanotoxins in sludge. MDPI 2022-10-31 /pmc/articles/PMC9697381/ /pubmed/36355999 http://dx.doi.org/10.3390/toxins14110749 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jalili, Farhad
Trigui, Hana
Maldonado, Juan Francisco Guerra
Dorner, Sarah
Zamyadi, Arash
Shapiro, B. Jesse
Terrat, Yves
Fortin, Nathalie
Sauvé, Sébastien
Prévost, Michèle
Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge
title Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge
title_full Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge
title_fullStr Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge
title_full_unstemmed Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge
title_short Impact of Stagnation on the Diversity of Cyanobacteria in Drinking Water Treatment Plant Sludge
title_sort impact of stagnation on the diversity of cyanobacteria in drinking water treatment plant sludge
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697381/
https://www.ncbi.nlm.nih.gov/pubmed/36355999
http://dx.doi.org/10.3390/toxins14110749
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