Micrococcus porci sp. nov., Isolated from Feces of Black Pig (Sus scrofa)

An aerobic bacterium, designated as strain KD337-16(T), was isolated from the fecal samples of a black pig. It exhibited spherical, non-motile and non–spore-forming, Gram-positive cells. KD337-16(T) was identified as a member of the genus Micrococcus through 16S rRNA gene sequencing, and its closest relatives were found to be Micrococcus endophyticus YIM 56238(T) (99.5% similarity), Micrococcus luteus NCTC 2665(T) (99.1%), Micrococcus yunnanensis YIM 65004(T) (99.1%), Micrococcus aloeverae AE-6(T) (99.1%), Micrococcus antarcticus T2(T) (98.9%), and Micrococcus flavus LW4(T) (98.7%). Phylogenomic trees were constructed, and strain KD337-16(T) was found to form its own cluster as an independent lineage of M. flavus LW4(T). Between KD337-16(T) and its close relatives, the average nucleotide identity, average amino acid identity, and digital DNA–DNA hybridization were below the respective species delineation thresholds at 82.1–86.6%, 78.1–86.1%, and 24.4–34.9%. The major cellular fatty acids and polar lipids were anteiso-C(15:0) and iso-C(15:0), and DPG and PG, respectively. The predominant menaquinone was MK-8(H(2)). Taken together, the results indicate that strain KD337-16(T) is a novel species of the genus Micrococcus, for which the name Micrococcus porci sp. nov. is proposed. The type strain is KD337-16(T) (=BCRC 81318(T) = NBRC 115578(T)).