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An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform

A freely available “in vitro dosimetry” web application is presented enabling users to predict the concentration of nanomaterials reaching the cell surface, and therefore available for attachment and internalization, from initial dispersion concentrations. The web application is based on the distort...

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Autores principales: Cheimarios, Nikolaos, Pem, Barbara, Tsoumanis, Andreas, Ilić, Krunoslav, Vrček, Ivana Vinković, Melagraki, Georgia, Bitounis, Dimitrios, Isigonis, Panagiotis, Dusinska, Maria, Lynch, Iseult, Demokritou, Philip, Afantitis, Antreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697428/
https://www.ncbi.nlm.nih.gov/pubmed/36432221
http://dx.doi.org/10.3390/nano12223935
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author Cheimarios, Nikolaos
Pem, Barbara
Tsoumanis, Andreas
Ilić, Krunoslav
Vrček, Ivana Vinković
Melagraki, Georgia
Bitounis, Dimitrios
Isigonis, Panagiotis
Dusinska, Maria
Lynch, Iseult
Demokritou, Philip
Afantitis, Antreas
author_facet Cheimarios, Nikolaos
Pem, Barbara
Tsoumanis, Andreas
Ilić, Krunoslav
Vrček, Ivana Vinković
Melagraki, Georgia
Bitounis, Dimitrios
Isigonis, Panagiotis
Dusinska, Maria
Lynch, Iseult
Demokritou, Philip
Afantitis, Antreas
author_sort Cheimarios, Nikolaos
collection PubMed
description A freely available “in vitro dosimetry” web application is presented enabling users to predict the concentration of nanomaterials reaching the cell surface, and therefore available for attachment and internalization, from initial dispersion concentrations. The web application is based on the distorted grid (DG) model for the dispersion of engineered nanoparticles (NPs) in culture medium used for in vitro cellular experiments, in accordance with previously published protocols for cellular dosimetry determination. A series of in vitro experiments for six different NPs, with Ag and Au cores, are performed to demonstrate the convenience of the web application for calculation of exposure concentrations of NPs. Our results show that the exposure concentrations at the cell surface can be more than 30 times higher compared to the nominal or dispersed concentrations, depending on the NPs’ properties and their behavior in the cell culture medium. Therefore, the importance of calculating the exposure concentration at the bottom of the cell culture wells used for in vitro arrays, i.e., the particle concentration at the cell surface, is clearly presented, and the tool introduced here allows users easy access to such calculations. Widespread application of this web tool will increase the reliability of subsequent toxicity data, allowing improved correlation of the real exposure concentration with the observed toxicity, enabling the hazard potentials of different NPs to be compared on a more robust basis.
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spelling pubmed-96974282022-11-26 An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform Cheimarios, Nikolaos Pem, Barbara Tsoumanis, Andreas Ilić, Krunoslav Vrček, Ivana Vinković Melagraki, Georgia Bitounis, Dimitrios Isigonis, Panagiotis Dusinska, Maria Lynch, Iseult Demokritou, Philip Afantitis, Antreas Nanomaterials (Basel) Article A freely available “in vitro dosimetry” web application is presented enabling users to predict the concentration of nanomaterials reaching the cell surface, and therefore available for attachment and internalization, from initial dispersion concentrations. The web application is based on the distorted grid (DG) model for the dispersion of engineered nanoparticles (NPs) in culture medium used for in vitro cellular experiments, in accordance with previously published protocols for cellular dosimetry determination. A series of in vitro experiments for six different NPs, with Ag and Au cores, are performed to demonstrate the convenience of the web application for calculation of exposure concentrations of NPs. Our results show that the exposure concentrations at the cell surface can be more than 30 times higher compared to the nominal or dispersed concentrations, depending on the NPs’ properties and their behavior in the cell culture medium. Therefore, the importance of calculating the exposure concentration at the bottom of the cell culture wells used for in vitro arrays, i.e., the particle concentration at the cell surface, is clearly presented, and the tool introduced here allows users easy access to such calculations. Widespread application of this web tool will increase the reliability of subsequent toxicity data, allowing improved correlation of the real exposure concentration with the observed toxicity, enabling the hazard potentials of different NPs to be compared on a more robust basis. MDPI 2022-11-08 /pmc/articles/PMC9697428/ /pubmed/36432221 http://dx.doi.org/10.3390/nano12223935 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cheimarios, Nikolaos
Pem, Barbara
Tsoumanis, Andreas
Ilić, Krunoslav
Vrček, Ivana Vinković
Melagraki, Georgia
Bitounis, Dimitrios
Isigonis, Panagiotis
Dusinska, Maria
Lynch, Iseult
Demokritou, Philip
Afantitis, Antreas
An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform
title An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform
title_full An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform
title_fullStr An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform
title_full_unstemmed An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform
title_short An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform
title_sort in vitro dosimetry tool for the numerical transport modeling of engineered nanomaterials powered by the enalos riskgone cloud platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697428/
https://www.ncbi.nlm.nih.gov/pubmed/36432221
http://dx.doi.org/10.3390/nano12223935
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