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Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A

The aim of this study was to determine the impact of vitamin A deficiency (VAD)/supplementation (±VA) and group A RV (RVA) maternal immunization of RVA seropositive multiparous pregnant sows, on their immune responses (anamnestic response) and on passive protection of their piglets against RVA chall...

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Autores principales: Chepngeno, Juliet, Amimo, Joshua O., Michael, Husheem, Jung, Kwonil, Raev, Sergei, Lee, Marcia V., Damtie, Debasu, Mainga, Alfred O., Vlasova, Anastasia N., Saif, Linda J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697517/
https://www.ncbi.nlm.nih.gov/pubmed/36366453
http://dx.doi.org/10.3390/v14112354
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author Chepngeno, Juliet
Amimo, Joshua O.
Michael, Husheem
Jung, Kwonil
Raev, Sergei
Lee, Marcia V.
Damtie, Debasu
Mainga, Alfred O.
Vlasova, Anastasia N.
Saif, Linda J.
author_facet Chepngeno, Juliet
Amimo, Joshua O.
Michael, Husheem
Jung, Kwonil
Raev, Sergei
Lee, Marcia V.
Damtie, Debasu
Mainga, Alfred O.
Vlasova, Anastasia N.
Saif, Linda J.
author_sort Chepngeno, Juliet
collection PubMed
description The aim of this study was to determine the impact of vitamin A deficiency (VAD)/supplementation (±VA) and group A RV (RVA) maternal immunization of RVA seropositive multiparous pregnant sows, on their immune responses (anamnestic response) and on passive protection of their piglets against RVA challenge. Our results showed that VAD- mock sows had increased RVA RNA shedding at 1–5 days post piglet RVA challenge, and their litters had increased RVA shedding and diarrhea frequency throughout the experiment. VAD decreased memory B cell frequencies while VA supplementation increased RVA specific IgA/IgG antibody (Ab) secreting cell (ASC) numbers in blood, milk, and tissues of RVA inoculated VAD sows. The increased numbers of RVA specific IgA/IgG ASCs in blood, milk/colostrum, intestinal contents, and tissues in VA supplemented VAD sows, suggest a role of VA in B cell immunity and trafficking to tissues. We also observed that RVA inoculated sows had the highest viral neutralizing Ab titers in serum and milk while VA supplementation of VAD sows and RVA inoculation increased IgA(+) B cell frequencies in sow colostrum. In summary, we demonstrated that daily oral VA-supplementation (2nd trimester-throughout lactation) to RVA inoculated VAD sows improved the function of their gut-mammary-IgA immunological axis, reducing viral RNA shedding, diarrhea, and increasing weight gain in suckling piglets.
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spelling pubmed-96975172022-11-26 Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A Chepngeno, Juliet Amimo, Joshua O. Michael, Husheem Jung, Kwonil Raev, Sergei Lee, Marcia V. Damtie, Debasu Mainga, Alfred O. Vlasova, Anastasia N. Saif, Linda J. Viruses Article The aim of this study was to determine the impact of vitamin A deficiency (VAD)/supplementation (±VA) and group A RV (RVA) maternal immunization of RVA seropositive multiparous pregnant sows, on their immune responses (anamnestic response) and on passive protection of their piglets against RVA challenge. Our results showed that VAD- mock sows had increased RVA RNA shedding at 1–5 days post piglet RVA challenge, and their litters had increased RVA shedding and diarrhea frequency throughout the experiment. VAD decreased memory B cell frequencies while VA supplementation increased RVA specific IgA/IgG antibody (Ab) secreting cell (ASC) numbers in blood, milk, and tissues of RVA inoculated VAD sows. The increased numbers of RVA specific IgA/IgG ASCs in blood, milk/colostrum, intestinal contents, and tissues in VA supplemented VAD sows, suggest a role of VA in B cell immunity and trafficking to tissues. We also observed that RVA inoculated sows had the highest viral neutralizing Ab titers in serum and milk while VA supplementation of VAD sows and RVA inoculation increased IgA(+) B cell frequencies in sow colostrum. In summary, we demonstrated that daily oral VA-supplementation (2nd trimester-throughout lactation) to RVA inoculated VAD sows improved the function of their gut-mammary-IgA immunological axis, reducing viral RNA shedding, diarrhea, and increasing weight gain in suckling piglets. MDPI 2022-10-26 /pmc/articles/PMC9697517/ /pubmed/36366453 http://dx.doi.org/10.3390/v14112354 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chepngeno, Juliet
Amimo, Joshua O.
Michael, Husheem
Jung, Kwonil
Raev, Sergei
Lee, Marcia V.
Damtie, Debasu
Mainga, Alfred O.
Vlasova, Anastasia N.
Saif, Linda J.
Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A
title Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A
title_full Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A
title_fullStr Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A
title_full_unstemmed Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A
title_short Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A
title_sort rotavirus a inoculation and oral vitamin a supplementation of vitamin a deficient pregnant sows enhances maternal adaptive immunity and passive protection of piglets against virulent rotavirus a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697517/
https://www.ncbi.nlm.nih.gov/pubmed/36366453
http://dx.doi.org/10.3390/v14112354
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