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The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice

Rice spotted-leaf mutants are ideal materials to study the molecular mechanism underlying programmed cell death and disease resistance in plants. LOC_Os07g04820 has previously been identified as the candidate gene responsible for the spotted-leaf phenotype in rice Spotted-leaf 26 (Spl26) mutant. Her...

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Autores principales: Shang, Huihui, Li, Panpan, Zhang, Xiaobo, Xu, Xia, Gong, Junyi, Yang, Shihua, He, Yuqing, Wu, Jian-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697700/
https://www.ncbi.nlm.nih.gov/pubmed/36430644
http://dx.doi.org/10.3390/ijms232214168
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author Shang, Huihui
Li, Panpan
Zhang, Xiaobo
Xu, Xia
Gong, Junyi
Yang, Shihua
He, Yuqing
Wu, Jian-Li
author_facet Shang, Huihui
Li, Panpan
Zhang, Xiaobo
Xu, Xia
Gong, Junyi
Yang, Shihua
He, Yuqing
Wu, Jian-Li
author_sort Shang, Huihui
collection PubMed
description Rice spotted-leaf mutants are ideal materials to study the molecular mechanism underlying programmed cell death and disease resistance in plants. LOC_Os07g04820 has previously been identified as the candidate gene responsible for the spotted-leaf phenotype in rice Spotted-leaf 26 (Spl26) mutant. Here, we cloned and validated that LOC_Os07g04820 is the locus controlling the spotted-leaf phenotype of Spl26 by reverse functional complementation and CRISPR/Cas9-mediated knockout of the mutant allele. The recessive wild-type spl26 allele (Oryza sativa spotted-leaf 26, Osspl26) is highly conservative in grass species and encodes a putative G-type lectin S-receptor-like serine/threonine protein kinase with 444 amino acid residuals. OsSPL26 localizes to the plasma membrane and can be detected constitutively in roots, stems, leaves, sheaths and panicles. The single base substitution from T to A at position 293 leads to phenylalanine/tyrosine replacement at position 98 in the encoded protein in the mutant and induces excessive accumulation of H(2)O(2), leading to oxidative damage to cells, and finally, formation of the spotted-leaf phenotype in Spl26. The formation of lesions not only affects the growth and development of the plants but also activates the defense response and enhances the resistance to the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae. Our results indicate that the gain-of-function by the mutant allele OsSpl26 positively regulates cell death and immunity in rice.
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spelling pubmed-96977002022-11-26 The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice Shang, Huihui Li, Panpan Zhang, Xiaobo Xu, Xia Gong, Junyi Yang, Shihua He, Yuqing Wu, Jian-Li Int J Mol Sci Article Rice spotted-leaf mutants are ideal materials to study the molecular mechanism underlying programmed cell death and disease resistance in plants. LOC_Os07g04820 has previously been identified as the candidate gene responsible for the spotted-leaf phenotype in rice Spotted-leaf 26 (Spl26) mutant. Here, we cloned and validated that LOC_Os07g04820 is the locus controlling the spotted-leaf phenotype of Spl26 by reverse functional complementation and CRISPR/Cas9-mediated knockout of the mutant allele. The recessive wild-type spl26 allele (Oryza sativa spotted-leaf 26, Osspl26) is highly conservative in grass species and encodes a putative G-type lectin S-receptor-like serine/threonine protein kinase with 444 amino acid residuals. OsSPL26 localizes to the plasma membrane and can be detected constitutively in roots, stems, leaves, sheaths and panicles. The single base substitution from T to A at position 293 leads to phenylalanine/tyrosine replacement at position 98 in the encoded protein in the mutant and induces excessive accumulation of H(2)O(2), leading to oxidative damage to cells, and finally, formation of the spotted-leaf phenotype in Spl26. The formation of lesions not only affects the growth and development of the plants but also activates the defense response and enhances the resistance to the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae. Our results indicate that the gain-of-function by the mutant allele OsSpl26 positively regulates cell death and immunity in rice. MDPI 2022-11-16 /pmc/articles/PMC9697700/ /pubmed/36430644 http://dx.doi.org/10.3390/ijms232214168 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shang, Huihui
Li, Panpan
Zhang, Xiaobo
Xu, Xia
Gong, Junyi
Yang, Shihua
He, Yuqing
Wu, Jian-Li
The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice
title The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice
title_full The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice
title_fullStr The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice
title_full_unstemmed The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice
title_short The Gain-of-Function Mutation, OsSpl26, Positively Regulates Plant Immunity in Rice
title_sort gain-of-function mutation, osspl26, positively regulates plant immunity in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697700/
https://www.ncbi.nlm.nih.gov/pubmed/36430644
http://dx.doi.org/10.3390/ijms232214168
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