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A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model
Ischemia-reperfusion injury remains a fundamental problem during organ transplantation logistics. One key technical factor is the rapid allograft rewarming during the time of vascular reconstruction in the recipient. In this pilot study, a new thermal insulation bag (TIB) for organ transplantation w...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697795/ https://www.ncbi.nlm.nih.gov/pubmed/36430283 http://dx.doi.org/10.3390/ijms232213806 |
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author | Ernst, Lisa Czigany, Zoltan Paschenda, Pascal Schulz, Mareike Breuer, Lukas Kunczik, Janosch Czaplik, Michael Liu, Wenjia Jiang, Decan Klinge, Uwe Djudjaj, Sonja Boor, Peter Lurje, Georg Kobayashi, Eiji Tolba, René H. |
author_facet | Ernst, Lisa Czigany, Zoltan Paschenda, Pascal Schulz, Mareike Breuer, Lukas Kunczik, Janosch Czaplik, Michael Liu, Wenjia Jiang, Decan Klinge, Uwe Djudjaj, Sonja Boor, Peter Lurje, Georg Kobayashi, Eiji Tolba, René H. |
author_sort | Ernst, Lisa |
collection | PubMed |
description | Ischemia-reperfusion injury remains a fundamental problem during organ transplantation logistics. One key technical factor is the rapid allograft rewarming during the time of vascular reconstruction in the recipient. In this pilot study, a new thermal insulation bag (TIB) for organ transplantation was used. Insulation capacity, tissue compatibility, and usability were tested initially ex vivo on porcine kidneys (n = 24) followed by the first in vivo usage. Fourteen female German landrace pigs underwent kidney auto-transplantation after 24 h cold storage (4 °C). During the implantation process the kidney was either insulated with the new TIB, or it was not thermo-protected at all, which represents the clinical standard. In this proof-of-concept study, the usability (knife-to-skin-time) and the general thermal capacity (30 min warm storage at 38 °C ex vivo p < 0.001) was shown. The clinical outcome showed significant differences in the determination of CRP and pi-GST levels. Syndecan-1 Antibody staining showed clear significant higher counts in the control group (p < 0.01) indicating epithelial damage. However, the effect on renal outcomes in not severely pre-damaged kidneys does not appear to be conclusively significant. A close follow-up study is warranted, especially in the context of marginal organs or in cases where anastomosis-times are prolonged due to surgical complexity (e.g., multiple vessels and complex reconstructions). |
format | Online Article Text |
id | pubmed-9697795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96977952022-11-26 A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model Ernst, Lisa Czigany, Zoltan Paschenda, Pascal Schulz, Mareike Breuer, Lukas Kunczik, Janosch Czaplik, Michael Liu, Wenjia Jiang, Decan Klinge, Uwe Djudjaj, Sonja Boor, Peter Lurje, Georg Kobayashi, Eiji Tolba, René H. Int J Mol Sci Article Ischemia-reperfusion injury remains a fundamental problem during organ transplantation logistics. One key technical factor is the rapid allograft rewarming during the time of vascular reconstruction in the recipient. In this pilot study, a new thermal insulation bag (TIB) for organ transplantation was used. Insulation capacity, tissue compatibility, and usability were tested initially ex vivo on porcine kidneys (n = 24) followed by the first in vivo usage. Fourteen female German landrace pigs underwent kidney auto-transplantation after 24 h cold storage (4 °C). During the implantation process the kidney was either insulated with the new TIB, or it was not thermo-protected at all, which represents the clinical standard. In this proof-of-concept study, the usability (knife-to-skin-time) and the general thermal capacity (30 min warm storage at 38 °C ex vivo p < 0.001) was shown. The clinical outcome showed significant differences in the determination of CRP and pi-GST levels. Syndecan-1 Antibody staining showed clear significant higher counts in the control group (p < 0.01) indicating epithelial damage. However, the effect on renal outcomes in not severely pre-damaged kidneys does not appear to be conclusively significant. A close follow-up study is warranted, especially in the context of marginal organs or in cases where anastomosis-times are prolonged due to surgical complexity (e.g., multiple vessels and complex reconstructions). MDPI 2022-11-09 /pmc/articles/PMC9697795/ /pubmed/36430283 http://dx.doi.org/10.3390/ijms232213806 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ernst, Lisa Czigany, Zoltan Paschenda, Pascal Schulz, Mareike Breuer, Lukas Kunczik, Janosch Czaplik, Michael Liu, Wenjia Jiang, Decan Klinge, Uwe Djudjaj, Sonja Boor, Peter Lurje, Georg Kobayashi, Eiji Tolba, René H. A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model |
title | A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model |
title_full | A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model |
title_fullStr | A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model |
title_full_unstemmed | A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model |
title_short | A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model |
title_sort | proof-of-concept preclinical study using a novel thermal insulation device in a porcine kidney auto-transplantation model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697795/ https://www.ncbi.nlm.nih.gov/pubmed/36430283 http://dx.doi.org/10.3390/ijms232213806 |
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