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A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model

Ischemia-reperfusion injury remains a fundamental problem during organ transplantation logistics. One key technical factor is the rapid allograft rewarming during the time of vascular reconstruction in the recipient. In this pilot study, a new thermal insulation bag (TIB) for organ transplantation w...

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Autores principales: Ernst, Lisa, Czigany, Zoltan, Paschenda, Pascal, Schulz, Mareike, Breuer, Lukas, Kunczik, Janosch, Czaplik, Michael, Liu, Wenjia, Jiang, Decan, Klinge, Uwe, Djudjaj, Sonja, Boor, Peter, Lurje, Georg, Kobayashi, Eiji, Tolba, René H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697795/
https://www.ncbi.nlm.nih.gov/pubmed/36430283
http://dx.doi.org/10.3390/ijms232213806
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author Ernst, Lisa
Czigany, Zoltan
Paschenda, Pascal
Schulz, Mareike
Breuer, Lukas
Kunczik, Janosch
Czaplik, Michael
Liu, Wenjia
Jiang, Decan
Klinge, Uwe
Djudjaj, Sonja
Boor, Peter
Lurje, Georg
Kobayashi, Eiji
Tolba, René H.
author_facet Ernst, Lisa
Czigany, Zoltan
Paschenda, Pascal
Schulz, Mareike
Breuer, Lukas
Kunczik, Janosch
Czaplik, Michael
Liu, Wenjia
Jiang, Decan
Klinge, Uwe
Djudjaj, Sonja
Boor, Peter
Lurje, Georg
Kobayashi, Eiji
Tolba, René H.
author_sort Ernst, Lisa
collection PubMed
description Ischemia-reperfusion injury remains a fundamental problem during organ transplantation logistics. One key technical factor is the rapid allograft rewarming during the time of vascular reconstruction in the recipient. In this pilot study, a new thermal insulation bag (TIB) for organ transplantation was used. Insulation capacity, tissue compatibility, and usability were tested initially ex vivo on porcine kidneys (n = 24) followed by the first in vivo usage. Fourteen female German landrace pigs underwent kidney auto-transplantation after 24 h cold storage (4 °C). During the implantation process the kidney was either insulated with the new TIB, or it was not thermo-protected at all, which represents the clinical standard. In this proof-of-concept study, the usability (knife-to-skin-time) and the general thermal capacity (30 min warm storage at 38 °C ex vivo p < 0.001) was shown. The clinical outcome showed significant differences in the determination of CRP and pi-GST levels. Syndecan-1 Antibody staining showed clear significant higher counts in the control group (p < 0.01) indicating epithelial damage. However, the effect on renal outcomes in not severely pre-damaged kidneys does not appear to be conclusively significant. A close follow-up study is warranted, especially in the context of marginal organs or in cases where anastomosis-times are prolonged due to surgical complexity (e.g., multiple vessels and complex reconstructions).
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spelling pubmed-96977952022-11-26 A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model Ernst, Lisa Czigany, Zoltan Paschenda, Pascal Schulz, Mareike Breuer, Lukas Kunczik, Janosch Czaplik, Michael Liu, Wenjia Jiang, Decan Klinge, Uwe Djudjaj, Sonja Boor, Peter Lurje, Georg Kobayashi, Eiji Tolba, René H. Int J Mol Sci Article Ischemia-reperfusion injury remains a fundamental problem during organ transplantation logistics. One key technical factor is the rapid allograft rewarming during the time of vascular reconstruction in the recipient. In this pilot study, a new thermal insulation bag (TIB) for organ transplantation was used. Insulation capacity, tissue compatibility, and usability were tested initially ex vivo on porcine kidneys (n = 24) followed by the first in vivo usage. Fourteen female German landrace pigs underwent kidney auto-transplantation after 24 h cold storage (4 °C). During the implantation process the kidney was either insulated with the new TIB, or it was not thermo-protected at all, which represents the clinical standard. In this proof-of-concept study, the usability (knife-to-skin-time) and the general thermal capacity (30 min warm storage at 38 °C ex vivo p < 0.001) was shown. The clinical outcome showed significant differences in the determination of CRP and pi-GST levels. Syndecan-1 Antibody staining showed clear significant higher counts in the control group (p < 0.01) indicating epithelial damage. However, the effect on renal outcomes in not severely pre-damaged kidneys does not appear to be conclusively significant. A close follow-up study is warranted, especially in the context of marginal organs or in cases where anastomosis-times are prolonged due to surgical complexity (e.g., multiple vessels and complex reconstructions). MDPI 2022-11-09 /pmc/articles/PMC9697795/ /pubmed/36430283 http://dx.doi.org/10.3390/ijms232213806 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ernst, Lisa
Czigany, Zoltan
Paschenda, Pascal
Schulz, Mareike
Breuer, Lukas
Kunczik, Janosch
Czaplik, Michael
Liu, Wenjia
Jiang, Decan
Klinge, Uwe
Djudjaj, Sonja
Boor, Peter
Lurje, Georg
Kobayashi, Eiji
Tolba, René H.
A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model
title A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model
title_full A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model
title_fullStr A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model
title_full_unstemmed A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model
title_short A Proof-of-Concept Preclinical Study Using a Novel Thermal Insulation Device in a Porcine Kidney Auto-Transplantation Model
title_sort proof-of-concept preclinical study using a novel thermal insulation device in a porcine kidney auto-transplantation model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697795/
https://www.ncbi.nlm.nih.gov/pubmed/36430283
http://dx.doi.org/10.3390/ijms232213806
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