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Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability

Rho1p is a membrane-associated protein that belongs to the Rho family of small GTPases. These proteins coordinate processes such as actin remodelling and polarised secretion to maintain the shape and homeostasis of yeast cells. In response to extracellular stimuli, Rho1p undergoes conformational swi...

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Autores principales: García, Patricia, Celador, Rubén, Pérez-Parrilla, Jorge, Sánchez, Yolanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697909/
https://www.ncbi.nlm.nih.gov/pubmed/36430366
http://dx.doi.org/10.3390/ijms232213888
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author García, Patricia
Celador, Rubén
Pérez-Parrilla, Jorge
Sánchez, Yolanda
author_facet García, Patricia
Celador, Rubén
Pérez-Parrilla, Jorge
Sánchez, Yolanda
author_sort García, Patricia
collection PubMed
description Rho1p is a membrane-associated protein that belongs to the Rho family of small GTPases. These proteins coordinate processes such as actin remodelling and polarised secretion to maintain the shape and homeostasis of yeast cells. In response to extracellular stimuli, Rho1p undergoes conformational switching between a guanosine triphosphate (GTP)-bound active state and a guanosine diphosphate (GDP)-bound inactive state. Cycling is improved with guanine nucleotide exchange factor (GEF) activity necessary to activate signalling and GTPase activating protein (GAP) activity required for subsequent signal depletion. This review focuses on fission yeast Rho1p GEFs, Rgf1p, Rgf2p, and Rgf3p that belong to the family of DH-PH domain-containing Dbl-related GEFs. They are multi-domain proteins that detect biological signals that induce or inhibit their catalytic activity over Rho1p. Each of them activates Rho1p in different places and times. Rgf1p acts preferentially during polarised growth. Rgf2p is required for sporulation, and Rgf3p plays an essential function in septum synthesis. In addition, we outline the noncanonical roles of Rho1p-GEFs in genomic instability.
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spelling pubmed-96979092022-11-26 Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability García, Patricia Celador, Rubén Pérez-Parrilla, Jorge Sánchez, Yolanda Int J Mol Sci Review Rho1p is a membrane-associated protein that belongs to the Rho family of small GTPases. These proteins coordinate processes such as actin remodelling and polarised secretion to maintain the shape and homeostasis of yeast cells. In response to extracellular stimuli, Rho1p undergoes conformational switching between a guanosine triphosphate (GTP)-bound active state and a guanosine diphosphate (GDP)-bound inactive state. Cycling is improved with guanine nucleotide exchange factor (GEF) activity necessary to activate signalling and GTPase activating protein (GAP) activity required for subsequent signal depletion. This review focuses on fission yeast Rho1p GEFs, Rgf1p, Rgf2p, and Rgf3p that belong to the family of DH-PH domain-containing Dbl-related GEFs. They are multi-domain proteins that detect biological signals that induce or inhibit their catalytic activity over Rho1p. Each of them activates Rho1p in different places and times. Rgf1p acts preferentially during polarised growth. Rgf2p is required for sporulation, and Rgf3p plays an essential function in septum synthesis. In addition, we outline the noncanonical roles of Rho1p-GEFs in genomic instability. MDPI 2022-11-11 /pmc/articles/PMC9697909/ /pubmed/36430366 http://dx.doi.org/10.3390/ijms232213888 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
García, Patricia
Celador, Rubén
Pérez-Parrilla, Jorge
Sánchez, Yolanda
Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability
title Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability
title_full Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability
title_fullStr Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability
title_full_unstemmed Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability
title_short Fission Yeast Rho1p-GEFs: From Polarity and Cell Wall Synthesis to Genome Stability
title_sort fission yeast rho1p-gefs: from polarity and cell wall synthesis to genome stability
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697909/
https://www.ncbi.nlm.nih.gov/pubmed/36430366
http://dx.doi.org/10.3390/ijms232213888
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