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Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry

The COVID-19 pandemic boosted the development of diagnostic tests to meet patient needs and provide accurate, sensitive, and fast disease detection. Despite rapid advancements, limitations related to turnaround time, varying performance metrics due to different sampling sites, illness duration, co-i...

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Autores principales: Moura, Alexandre Varao, de Oliveira, Danilo Cardoso, Silva, Alex Ap. R., da Rosa, Jonas Ribeiro, Garcia, Pedro Henrique Dias, Sanches, Pedro Henrique Godoy, Garza, Kyana Y., Mendes, Flavio Marcio Macedo, Lambert, Mayara, Gutierrez, Junier Marrero, Granado, Nicole Marino, dos Santos, Alicia Camacho, de Lima, Iasmim Lopes, Negrini, Lisamara Dias de Oliveira, Antonio, Marcia Aparecida, Eberlin, Marcos N., Eberlin, Livia S., Porcari, Andreia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697918/
https://www.ncbi.nlm.nih.gov/pubmed/36355139
http://dx.doi.org/10.3390/metabo12111056
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author Moura, Alexandre Varao
de Oliveira, Danilo Cardoso
Silva, Alex Ap. R.
da Rosa, Jonas Ribeiro
Garcia, Pedro Henrique Dias
Sanches, Pedro Henrique Godoy
Garza, Kyana Y.
Mendes, Flavio Marcio Macedo
Lambert, Mayara
Gutierrez, Junier Marrero
Granado, Nicole Marino
dos Santos, Alicia Camacho
de Lima, Iasmim Lopes
Negrini, Lisamara Dias de Oliveira
Antonio, Marcia Aparecida
Eberlin, Marcos N.
Eberlin, Livia S.
Porcari, Andreia M.
author_facet Moura, Alexandre Varao
de Oliveira, Danilo Cardoso
Silva, Alex Ap. R.
da Rosa, Jonas Ribeiro
Garcia, Pedro Henrique Dias
Sanches, Pedro Henrique Godoy
Garza, Kyana Y.
Mendes, Flavio Marcio Macedo
Lambert, Mayara
Gutierrez, Junier Marrero
Granado, Nicole Marino
dos Santos, Alicia Camacho
de Lima, Iasmim Lopes
Negrini, Lisamara Dias de Oliveira
Antonio, Marcia Aparecida
Eberlin, Marcos N.
Eberlin, Livia S.
Porcari, Andreia M.
author_sort Moura, Alexandre Varao
collection PubMed
description The COVID-19 pandemic boosted the development of diagnostic tests to meet patient needs and provide accurate, sensitive, and fast disease detection. Despite rapid advancements, limitations related to turnaround time, varying performance metrics due to different sampling sites, illness duration, co-infections, and the need for particular reagents still exist. As an alternative diagnostic test, we present urine analysis through flow-injection–tandem mass spectrometry (FIA-MS/MS) as a powerful approach for COVID-19 diagnosis, targeting the detection of amino acids and acylcarnitines. We adapted a method that is widely used for newborn screening tests on dried blood for urine samples in order to detect metabolites related to COVID-19 infection. We analyzed samples from 246 volunteers with diagnostic confirmation via PCR. Urine samples were self-collected, diluted, and analyzed with a run time of 4 min. A Lasso statistical classifier was built using 75/25% data for training/validation sets and achieved high diagnostic performances: 97/90% sensitivity, 95/100% specificity, and 95/97.2% accuracy. Additionally, we predicted on two withheld sets composed of suspected hospitalized/symptomatic COVID-19-PCR negative patients and patients out of the optimal time-frame collection for PCR diagnosis, with promising results. Altogether, we show that the benchmarked FIA-MS/MS method is promising for COVID-19 screening and diagnosis, and is also potentially useful after the peak viral load has passed.
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spelling pubmed-96979182022-11-26 Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry Moura, Alexandre Varao de Oliveira, Danilo Cardoso Silva, Alex Ap. R. da Rosa, Jonas Ribeiro Garcia, Pedro Henrique Dias Sanches, Pedro Henrique Godoy Garza, Kyana Y. Mendes, Flavio Marcio Macedo Lambert, Mayara Gutierrez, Junier Marrero Granado, Nicole Marino dos Santos, Alicia Camacho de Lima, Iasmim Lopes Negrini, Lisamara Dias de Oliveira Antonio, Marcia Aparecida Eberlin, Marcos N. Eberlin, Livia S. Porcari, Andreia M. Metabolites Article The COVID-19 pandemic boosted the development of diagnostic tests to meet patient needs and provide accurate, sensitive, and fast disease detection. Despite rapid advancements, limitations related to turnaround time, varying performance metrics due to different sampling sites, illness duration, co-infections, and the need for particular reagents still exist. As an alternative diagnostic test, we present urine analysis through flow-injection–tandem mass spectrometry (FIA-MS/MS) as a powerful approach for COVID-19 diagnosis, targeting the detection of amino acids and acylcarnitines. We adapted a method that is widely used for newborn screening tests on dried blood for urine samples in order to detect metabolites related to COVID-19 infection. We analyzed samples from 246 volunteers with diagnostic confirmation via PCR. Urine samples were self-collected, diluted, and analyzed with a run time of 4 min. A Lasso statistical classifier was built using 75/25% data for training/validation sets and achieved high diagnostic performances: 97/90% sensitivity, 95/100% specificity, and 95/97.2% accuracy. Additionally, we predicted on two withheld sets composed of suspected hospitalized/symptomatic COVID-19-PCR negative patients and patients out of the optimal time-frame collection for PCR diagnosis, with promising results. Altogether, we show that the benchmarked FIA-MS/MS method is promising for COVID-19 screening and diagnosis, and is also potentially useful after the peak viral load has passed. MDPI 2022-11-02 /pmc/articles/PMC9697918/ /pubmed/36355139 http://dx.doi.org/10.3390/metabo12111056 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moura, Alexandre Varao
de Oliveira, Danilo Cardoso
Silva, Alex Ap. R.
da Rosa, Jonas Ribeiro
Garcia, Pedro Henrique Dias
Sanches, Pedro Henrique Godoy
Garza, Kyana Y.
Mendes, Flavio Marcio Macedo
Lambert, Mayara
Gutierrez, Junier Marrero
Granado, Nicole Marino
dos Santos, Alicia Camacho
de Lima, Iasmim Lopes
Negrini, Lisamara Dias de Oliveira
Antonio, Marcia Aparecida
Eberlin, Marcos N.
Eberlin, Livia S.
Porcari, Andreia M.
Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry
title Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry
title_full Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry
title_fullStr Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry
title_full_unstemmed Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry
title_short Urine Metabolites Enable Fast Detection of COVID-19 Using Mass Spectrometry
title_sort urine metabolites enable fast detection of covid-19 using mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697918/
https://www.ncbi.nlm.nih.gov/pubmed/36355139
http://dx.doi.org/10.3390/metabo12111056
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