Cargando…

The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control

Although much is known about seed and fruit development at the molecular level, many gaps remain in our understanding of how cell wall modifications can impact developmental processes in plants, as well as how biomechanical alterations influence seed and fruit growth. Mutants of Arabidopsis thaliana...

Descripción completa

Detalles Bibliográficos
Autores principales: Di Marzo, Maurizio, Babolin, Nicola, Viana, Vívian Ebeling, de Oliveira, Antonio Costa, Gugi, Bruno, Caporali, Elisabetta, Herrera-Ubaldo, Humberto, Martínez-Estrada, Eduardo, Driouich, Azeddine, de Folter, Stefan, Colombo, Lucia, Ezquer, Ignacio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698089/
https://www.ncbi.nlm.nih.gov/pubmed/36432874
http://dx.doi.org/10.3390/plants11223146
_version_ 1784838728966995968
author Di Marzo, Maurizio
Babolin, Nicola
Viana, Vívian Ebeling
de Oliveira, Antonio Costa
Gugi, Bruno
Caporali, Elisabetta
Herrera-Ubaldo, Humberto
Martínez-Estrada, Eduardo
Driouich, Azeddine
de Folter, Stefan
Colombo, Lucia
Ezquer, Ignacio
author_facet Di Marzo, Maurizio
Babolin, Nicola
Viana, Vívian Ebeling
de Oliveira, Antonio Costa
Gugi, Bruno
Caporali, Elisabetta
Herrera-Ubaldo, Humberto
Martínez-Estrada, Eduardo
Driouich, Azeddine
de Folter, Stefan
Colombo, Lucia
Ezquer, Ignacio
author_sort Di Marzo, Maurizio
collection PubMed
description Although much is known about seed and fruit development at the molecular level, many gaps remain in our understanding of how cell wall modifications can impact developmental processes in plants, as well as how biomechanical alterations influence seed and fruit growth. Mutants of Arabidopsis thaliana constitute an excellent tool to study the function of gene families devoted to cell wall biogenesis. We have characterized a collection of lines carrying mutations in representative cell wall-related genes for seed and fruit size developmental defects, as well as altered germination rates. We have linked these studies to cell wall composition and structure. Interestingly, we have found that disruption of genes involved in pectin maturation and hemicellulose deposition strongly influence germination dynamics. Finally, we focused on two transcriptional regulators, SEEDSTICK (STK) and LEUNIG-HOMOLOG (LUH), which positively regulate seed growth. Herein, we demonstrate that these factors regulate specific aspects of cell wall properties such as pectin distribution. We propose a model wherein changes in seed coat structure due to alterations in the xyloglucan-cellulose matrix deposition and pectin maturation are critical for organ growth and germination. The results demonstrate the importance of cell wall properties and remodeling of polysaccharides as major factors responsible for seed development.
format Online
Article
Text
id pubmed-9698089
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96980892022-11-26 The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control Di Marzo, Maurizio Babolin, Nicola Viana, Vívian Ebeling de Oliveira, Antonio Costa Gugi, Bruno Caporali, Elisabetta Herrera-Ubaldo, Humberto Martínez-Estrada, Eduardo Driouich, Azeddine de Folter, Stefan Colombo, Lucia Ezquer, Ignacio Plants (Basel) Article Although much is known about seed and fruit development at the molecular level, many gaps remain in our understanding of how cell wall modifications can impact developmental processes in plants, as well as how biomechanical alterations influence seed and fruit growth. Mutants of Arabidopsis thaliana constitute an excellent tool to study the function of gene families devoted to cell wall biogenesis. We have characterized a collection of lines carrying mutations in representative cell wall-related genes for seed and fruit size developmental defects, as well as altered germination rates. We have linked these studies to cell wall composition and structure. Interestingly, we have found that disruption of genes involved in pectin maturation and hemicellulose deposition strongly influence germination dynamics. Finally, we focused on two transcriptional regulators, SEEDSTICK (STK) and LEUNIG-HOMOLOG (LUH), which positively regulate seed growth. Herein, we demonstrate that these factors regulate specific aspects of cell wall properties such as pectin distribution. We propose a model wherein changes in seed coat structure due to alterations in the xyloglucan-cellulose matrix deposition and pectin maturation are critical for organ growth and germination. The results demonstrate the importance of cell wall properties and remodeling of polysaccharides as major factors responsible for seed development. MDPI 2022-11-17 /pmc/articles/PMC9698089/ /pubmed/36432874 http://dx.doi.org/10.3390/plants11223146 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Marzo, Maurizio
Babolin, Nicola
Viana, Vívian Ebeling
de Oliveira, Antonio Costa
Gugi, Bruno
Caporali, Elisabetta
Herrera-Ubaldo, Humberto
Martínez-Estrada, Eduardo
Driouich, Azeddine
de Folter, Stefan
Colombo, Lucia
Ezquer, Ignacio
The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control
title The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control
title_full The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control
title_fullStr The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control
title_full_unstemmed The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control
title_short The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control
title_sort genetic control of seedstick and leunig-homolog in seed and fruit development: new insights into cell wall control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698089/
https://www.ncbi.nlm.nih.gov/pubmed/36432874
http://dx.doi.org/10.3390/plants11223146
work_keys_str_mv AT dimarzomaurizio thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT babolinnicola thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT vianavivianebeling thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT deoliveiraantoniocosta thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT gugibruno thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT caporalielisabetta thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT herreraubaldohumberto thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT martinezestradaeduardo thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT driouichazeddine thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT defolterstefan thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT colombolucia thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT ezquerignacio thegeneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT dimarzomaurizio geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT babolinnicola geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT vianavivianebeling geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT deoliveiraantoniocosta geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT gugibruno geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT caporalielisabetta geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT herreraubaldohumberto geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT martinezestradaeduardo geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT driouichazeddine geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT defolterstefan geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT colombolucia geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol
AT ezquerignacio geneticcontrolofseedstickandleunighomologinseedandfruitdevelopmentnewinsightsintocellwallcontrol