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Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family

Rondeletia odorata Jacquin is a flowering plant that belongs to the coffee family. As a rich source of polyphenols with significant antioxidant potential, R. odorata may have health benefits. Therefore, in the current work, ethanolic extract of aerial parts and its n-hexane, ethyl acetate, and n-but...

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Autores principales: Khursheed, Anjum, Ahmad, Saeed, Saleem, Muhammad, Khan, Kashif-ur-Rehman, Khan, Jallat, Orhan, Ilkay Erdogan, Abaci, Nurten, Imran, Muhammad, Tauseef, Saba, Uddin, Reaz, Yawer, Mirza Arfan, Tousif, Muhammad Imran, Ojha, Suvash Chandra, Khurshid, Umair
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698119/
https://www.ncbi.nlm.nih.gov/pubmed/36438873
http://dx.doi.org/10.3389/fchem.2022.1017577
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author Khursheed, Anjum
Ahmad, Saeed
Saleem, Muhammad
Khan, Kashif-ur-Rehman
Khan, Jallat
Orhan, Ilkay Erdogan
Abaci, Nurten
Imran, Muhammad
Tauseef, Saba
Uddin, Reaz
Yawer, Mirza Arfan
Tousif, Muhammad Imran
Ojha, Suvash Chandra
Khurshid, Umair
author_facet Khursheed, Anjum
Ahmad, Saeed
Saleem, Muhammad
Khan, Kashif-ur-Rehman
Khan, Jallat
Orhan, Ilkay Erdogan
Abaci, Nurten
Imran, Muhammad
Tauseef, Saba
Uddin, Reaz
Yawer, Mirza Arfan
Tousif, Muhammad Imran
Ojha, Suvash Chandra
Khurshid, Umair
author_sort Khursheed, Anjum
collection PubMed
description Rondeletia odorata Jacquin is a flowering plant that belongs to the coffee family. As a rich source of polyphenols with significant antioxidant potential, R. odorata may have health benefits. Therefore, in the current work, ethanolic extract of aerial parts and its n-hexane, ethyl acetate, and n-butanol soluble fractions were analyzed for their antioxidant potential and various enzyme inhibition properties. The total phenolic and flavonoid contents of the crude ethanol extract (ROE) and its n-hexane (ROH), ethyl acetate (ROEA), and n-butanol (ROB) fractions were determined spectrophotometrically, while metabolic profiling was established through UHPLC-MS analysis, which revealed the presence of 58 phytochemicals. Total phenolic and flavonoid contents of ROE extract were measured as 51.92 mg GA.Eq./g of dry extract and 52.35 mg Qu.Eq./g of the dry extract, respectively. In the DPPH radical scavenging activity assay, ROE and ROEA showed the highest potential with values of 62.13 ± 0.62 and 76.31% ± 1.86%, respectively, comparable to quercetin (80.89% ± 0.54%). Similarly, in the FRAP assay, the same pattern of the activity was observed with ROE and ROEA, which displayed absorbance values of 1.32 ± 0.01 and 0.80 ± 0.02 at 700 nm, respectively, which are comparable (1.76 ± 0.02) with the reference compound quercetin, whereas the ROH showed maximum metal-chelating capacity (62.61% ± 1.01%) among all extracts and fractions. Antibacterial activity assay indicated that the ROEA fraction was the most active against Serratia marcescens, Stenotrophomonas maltophilia, Bacillus subtilis, Klebsiella pneumonia, and Staphylococcus aureus, while the rest of the fractions showed good to moderate activity. Enzyme inhibition assays showed that ROEA fraction exhibited the highest activity with IC(50) values of 2.78 ± 0.42 and 3.95 ± 0.13 mg/mL against urease and carbonic anhydrase (CA), respectively. Furthermore, the docking studies of some of the major compounds identified in the extract revealed a strong correlation with their inhibitory activity. All extracts and fractions were also tested for their thrombolytic activity, and the ROB fraction showed a notable potential. Antiviral assay led to remarkable outcomes. Thus, it can be inferred that aerial parts of R. odorata are potential sources of bioactive components with several significant pharmacological activities.
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spelling pubmed-96981192022-11-26 Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family Khursheed, Anjum Ahmad, Saeed Saleem, Muhammad Khan, Kashif-ur-Rehman Khan, Jallat Orhan, Ilkay Erdogan Abaci, Nurten Imran, Muhammad Tauseef, Saba Uddin, Reaz Yawer, Mirza Arfan Tousif, Muhammad Imran Ojha, Suvash Chandra Khurshid, Umair Front Chem Chemistry Rondeletia odorata Jacquin is a flowering plant that belongs to the coffee family. As a rich source of polyphenols with significant antioxidant potential, R. odorata may have health benefits. Therefore, in the current work, ethanolic extract of aerial parts and its n-hexane, ethyl acetate, and n-butanol soluble fractions were analyzed for their antioxidant potential and various enzyme inhibition properties. The total phenolic and flavonoid contents of the crude ethanol extract (ROE) and its n-hexane (ROH), ethyl acetate (ROEA), and n-butanol (ROB) fractions were determined spectrophotometrically, while metabolic profiling was established through UHPLC-MS analysis, which revealed the presence of 58 phytochemicals. Total phenolic and flavonoid contents of ROE extract were measured as 51.92 mg GA.Eq./g of dry extract and 52.35 mg Qu.Eq./g of the dry extract, respectively. In the DPPH radical scavenging activity assay, ROE and ROEA showed the highest potential with values of 62.13 ± 0.62 and 76.31% ± 1.86%, respectively, comparable to quercetin (80.89% ± 0.54%). Similarly, in the FRAP assay, the same pattern of the activity was observed with ROE and ROEA, which displayed absorbance values of 1.32 ± 0.01 and 0.80 ± 0.02 at 700 nm, respectively, which are comparable (1.76 ± 0.02) with the reference compound quercetin, whereas the ROH showed maximum metal-chelating capacity (62.61% ± 1.01%) among all extracts and fractions. Antibacterial activity assay indicated that the ROEA fraction was the most active against Serratia marcescens, Stenotrophomonas maltophilia, Bacillus subtilis, Klebsiella pneumonia, and Staphylococcus aureus, while the rest of the fractions showed good to moderate activity. Enzyme inhibition assays showed that ROEA fraction exhibited the highest activity with IC(50) values of 2.78 ± 0.42 and 3.95 ± 0.13 mg/mL against urease and carbonic anhydrase (CA), respectively. Furthermore, the docking studies of some of the major compounds identified in the extract revealed a strong correlation with their inhibitory activity. All extracts and fractions were also tested for their thrombolytic activity, and the ROB fraction showed a notable potential. Antiviral assay led to remarkable outcomes. Thus, it can be inferred that aerial parts of R. odorata are potential sources of bioactive components with several significant pharmacological activities. Frontiers Media S.A. 2022-11-11 /pmc/articles/PMC9698119/ /pubmed/36438873 http://dx.doi.org/10.3389/fchem.2022.1017577 Text en Copyright © 2022 Khursheed, Ahmad, Saleem, Khan, Khan, Orhan, Abaci, Imran, Tauseef, Uddin, Yawer, Tousif, Ojha and Khurshid. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Khursheed, Anjum
Ahmad, Saeed
Saleem, Muhammad
Khan, Kashif-ur-Rehman
Khan, Jallat
Orhan, Ilkay Erdogan
Abaci, Nurten
Imran, Muhammad
Tauseef, Saba
Uddin, Reaz
Yawer, Mirza Arfan
Tousif, Muhammad Imran
Ojha, Suvash Chandra
Khurshid, Umair
Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family
title Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family
title_full Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family
title_fullStr Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family
title_full_unstemmed Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family
title_short Phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of Rondeletia odorata Jacquin: An unexplored plant of the coffee family
title_sort phytochemical profiling, in vitro biological activity, docking studies, and cytotoxicity assessments of rondeletia odorata jacquin: an unexplored plant of the coffee family
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698119/
https://www.ncbi.nlm.nih.gov/pubmed/36438873
http://dx.doi.org/10.3389/fchem.2022.1017577
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