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Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats
Exogenous corticosterone administration reduces GABAergic transmission and impairs its 5-HT(7) receptor-dependent modulation in the rat dorsal raphe nucleus (DRN), but it is largely unknown how neuronal functions of the DRN are affected by repeated physical and psychological stress. This study compa...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698125/ https://www.ncbi.nlm.nih.gov/pubmed/36430779 http://dx.doi.org/10.3390/ijms232214303 |
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author | Bąk, Joanna Bobula, Bartosz Hess, Grzegorz |
author_facet | Bąk, Joanna Bobula, Bartosz Hess, Grzegorz |
author_sort | Bąk, Joanna |
collection | PubMed |
description | Exogenous corticosterone administration reduces GABAergic transmission and impairs its 5-HT(7) receptor-dependent modulation in the rat dorsal raphe nucleus (DRN), but it is largely unknown how neuronal functions of the DRN are affected by repeated physical and psychological stress. This study compared the effects of repeated restraint stress and corticosterone injections on DRN neuronal excitability, spontaneous synaptic transmission, and its 5-HT(7) receptor-dependent modulation. Male Wistar rats received corticosterone injections for 7 or 14 days or were restrained for 10 min twice daily for 3 days. Repeated restraint stress and repeated corticosterone administration evoked similar changes in performance in the forced swim test. They increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) recorded from DRN neurons. In contrast to the treatment with corticosterone, restraint stress-induced changes in sEPSC kinetics and decreased intrinsic excitability of DRN neurons did not modify inhibitory transmission. Repeated injections of the 5-HT(7) receptor antagonist SB 269970 ameliorated the effects of restraint on excitability and sEPSC frequency but did not restore the altered kinetics of sEPSCs. Thus, repeated restraint stress and repeated corticosterone administration differ in consequences for the intrinsic excitability of DRN projection neurons and their excitatory and inhibitory synaptic inputs. Effects of repeated restraint stress on DRN neurons can be partially abrogated by blocking the 5-HT(7) receptor. |
format | Online Article Text |
id | pubmed-9698125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96981252022-11-26 Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats Bąk, Joanna Bobula, Bartosz Hess, Grzegorz Int J Mol Sci Article Exogenous corticosterone administration reduces GABAergic transmission and impairs its 5-HT(7) receptor-dependent modulation in the rat dorsal raphe nucleus (DRN), but it is largely unknown how neuronal functions of the DRN are affected by repeated physical and psychological stress. This study compared the effects of repeated restraint stress and corticosterone injections on DRN neuronal excitability, spontaneous synaptic transmission, and its 5-HT(7) receptor-dependent modulation. Male Wistar rats received corticosterone injections for 7 or 14 days or were restrained for 10 min twice daily for 3 days. Repeated restraint stress and repeated corticosterone administration evoked similar changes in performance in the forced swim test. They increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) recorded from DRN neurons. In contrast to the treatment with corticosterone, restraint stress-induced changes in sEPSC kinetics and decreased intrinsic excitability of DRN neurons did not modify inhibitory transmission. Repeated injections of the 5-HT(7) receptor antagonist SB 269970 ameliorated the effects of restraint on excitability and sEPSC frequency but did not restore the altered kinetics of sEPSCs. Thus, repeated restraint stress and repeated corticosterone administration differ in consequences for the intrinsic excitability of DRN projection neurons and their excitatory and inhibitory synaptic inputs. Effects of repeated restraint stress on DRN neurons can be partially abrogated by blocking the 5-HT(7) receptor. MDPI 2022-11-18 /pmc/articles/PMC9698125/ /pubmed/36430779 http://dx.doi.org/10.3390/ijms232214303 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bąk, Joanna Bobula, Bartosz Hess, Grzegorz Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats |
title | Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats |
title_full | Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats |
title_fullStr | Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats |
title_full_unstemmed | Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats |
title_short | Restraint Stress and Repeated Corticosterone Administration Differentially Affect Neuronal Excitability, Synaptic Transmission and 5-HT(7) Receptor Reactivity in the Dorsal Raphe Nucleus of Young Adult Male Rats |
title_sort | restraint stress and repeated corticosterone administration differentially affect neuronal excitability, synaptic transmission and 5-ht(7) receptor reactivity in the dorsal raphe nucleus of young adult male rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698125/ https://www.ncbi.nlm.nih.gov/pubmed/36430779 http://dx.doi.org/10.3390/ijms232214303 |
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