Cargando…

Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model

We utilised a ground-based microgravity hindlimb unloading (HU) mouse model to elucidate the gut microbiota bacterial changes in mice under a simulated microgravity environment. Four-month-old, male C57/Bl6 mice were randomly divided into ground-based controls and the HU groups and kept under contro...

Descripción completa

Detalles Bibliográficos
Autores principales: Siddiqui, Ruqaiyyah, Qaisar, Rizwan, Khan, Naveed Ahmed, Alharbi, Ahmad M., Alfahemi, Hasan, Elmoselhi, Adel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698145/
https://www.ncbi.nlm.nih.gov/pubmed/36431000
http://dx.doi.org/10.3390/life12111865
_version_ 1784838743228678144
author Siddiqui, Ruqaiyyah
Qaisar, Rizwan
Khan, Naveed Ahmed
Alharbi, Ahmad M.
Alfahemi, Hasan
Elmoselhi, Adel
author_facet Siddiqui, Ruqaiyyah
Qaisar, Rizwan
Khan, Naveed Ahmed
Alharbi, Ahmad M.
Alfahemi, Hasan
Elmoselhi, Adel
author_sort Siddiqui, Ruqaiyyah
collection PubMed
description We utilised a ground-based microgravity hindlimb unloading (HU) mouse model to elucidate the gut microbiota bacterial changes in mice under a simulated microgravity environment. Four-month-old, male C57/Bl6 mice were randomly divided into ground-based controls and the HU groups and kept under controlled environmental conditions. For the microgravity environment, the mice were suspended in special cages individually for 20 days. At the end of the suspension, the mice were sacrificed; gut dissections were performed, followed by a metagenomic analysis of bacterial species, which was carried out by extracting DNA and 16S rRNA analysis. The results revealed that the gut bacterial communities of mice under gravity and microgravity were different. Notably, our findings revealed differences in the bacterial community structure. Around 449 bacterial OTUs were specific to mice kept under normal gravity versus 443 bacterial OTUs under microgravity conditions. In contrast, 694 bacterial OTUs were common to both groups. When the relative abundance of taxa was analyzed, Bacteroidetes dominated the gut (64.7%) of normal mice. Conversely, mice in the microgravity environment were dominated by Firmicutes (42.7%), and the relative abundance of Bacteroidetes differed significantly between the two groups (p < 0.05). The distribution of Muribaculaceae between normal mice versus microgravity mice was significantly different, at 62% and 36.4%, respectively (p < 0.05). Furthermore, a significant decrease in 11 bacteria was observed in mice under simulated microgravity, including Akkermansia muciniphila, Eubacterium coprostanoligenes, Bacteroides acidifaciens, Clostridium leptum, Methylorubrum extorquens, Comamonas testosterone, Desulfovibrio fairfieldensis, Bacteroides coprocola, Aerococcus urinaeequi, Helicobacter hepaticus, and Burkholderiales. Further studies are needed to elucidate gut bacterial metabolites of these identified bacterial species in microgravity conditions and normal environment. Notably, the influence of these metabolites on obesity, neuroprotection, musculoskeletal and cardiovascular dysfunction, longevity, inflammation, health, and disease in astronauts ought to be investigated and will be important in developing procedures against adverse effects in astronauts following space travel.
format Online
Article
Text
id pubmed-9698145
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96981452022-11-26 Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model Siddiqui, Ruqaiyyah Qaisar, Rizwan Khan, Naveed Ahmed Alharbi, Ahmad M. Alfahemi, Hasan Elmoselhi, Adel Life (Basel) Article We utilised a ground-based microgravity hindlimb unloading (HU) mouse model to elucidate the gut microbiota bacterial changes in mice under a simulated microgravity environment. Four-month-old, male C57/Bl6 mice were randomly divided into ground-based controls and the HU groups and kept under controlled environmental conditions. For the microgravity environment, the mice were suspended in special cages individually for 20 days. At the end of the suspension, the mice were sacrificed; gut dissections were performed, followed by a metagenomic analysis of bacterial species, which was carried out by extracting DNA and 16S rRNA analysis. The results revealed that the gut bacterial communities of mice under gravity and microgravity were different. Notably, our findings revealed differences in the bacterial community structure. Around 449 bacterial OTUs were specific to mice kept under normal gravity versus 443 bacterial OTUs under microgravity conditions. In contrast, 694 bacterial OTUs were common to both groups. When the relative abundance of taxa was analyzed, Bacteroidetes dominated the gut (64.7%) of normal mice. Conversely, mice in the microgravity environment were dominated by Firmicutes (42.7%), and the relative abundance of Bacteroidetes differed significantly between the two groups (p < 0.05). The distribution of Muribaculaceae between normal mice versus microgravity mice was significantly different, at 62% and 36.4%, respectively (p < 0.05). Furthermore, a significant decrease in 11 bacteria was observed in mice under simulated microgravity, including Akkermansia muciniphila, Eubacterium coprostanoligenes, Bacteroides acidifaciens, Clostridium leptum, Methylorubrum extorquens, Comamonas testosterone, Desulfovibrio fairfieldensis, Bacteroides coprocola, Aerococcus urinaeequi, Helicobacter hepaticus, and Burkholderiales. Further studies are needed to elucidate gut bacterial metabolites of these identified bacterial species in microgravity conditions and normal environment. Notably, the influence of these metabolites on obesity, neuroprotection, musculoskeletal and cardiovascular dysfunction, longevity, inflammation, health, and disease in astronauts ought to be investigated and will be important in developing procedures against adverse effects in astronauts following space travel. MDPI 2022-11-12 /pmc/articles/PMC9698145/ /pubmed/36431000 http://dx.doi.org/10.3390/life12111865 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Siddiqui, Ruqaiyyah
Qaisar, Rizwan
Khan, Naveed Ahmed
Alharbi, Ahmad M.
Alfahemi, Hasan
Elmoselhi, Adel
Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model
title Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model
title_full Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model
title_fullStr Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model
title_full_unstemmed Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model
title_short Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model
title_sort effect of microgravity on the gut microbiota bacterial composition in a hindlimb unloading model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698145/
https://www.ncbi.nlm.nih.gov/pubmed/36431000
http://dx.doi.org/10.3390/life12111865
work_keys_str_mv AT siddiquiruqaiyyah effectofmicrogravityonthegutmicrobiotabacterialcompositioninahindlimbunloadingmodel
AT qaisarrizwan effectofmicrogravityonthegutmicrobiotabacterialcompositioninahindlimbunloadingmodel
AT khannaveedahmed effectofmicrogravityonthegutmicrobiotabacterialcompositioninahindlimbunloadingmodel
AT alharbiahmadm effectofmicrogravityonthegutmicrobiotabacterialcompositioninahindlimbunloadingmodel
AT alfahemihasan effectofmicrogravityonthegutmicrobiotabacterialcompositioninahindlimbunloadingmodel
AT elmoselhiadel effectofmicrogravityonthegutmicrobiotabacterialcompositioninahindlimbunloadingmodel