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Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts

Grafting polyethylene glycol (PEG) onto a polymer’s surface is widely used to improve biocompatibility by reducing protein and cell adhesion. Although PEG is considered to be bioinert, its incorporation onto biomaterials has shown to improve cell viability depending on the amount and molecular weigh...

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Autores principales: Abreu-Rejón, Antonio David, Herrera-Kao, Wilberth Antonio, May-Pat, Alejandro, Ávila-Ortega, Alejandro, Rodríguez-Fuentes, Nayeli, Uribe-Calderón, Jorge Alonso, Cervantes-Uc, José Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698172/
https://www.ncbi.nlm.nih.gov/pubmed/36433040
http://dx.doi.org/10.3390/polym14224912
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author Abreu-Rejón, Antonio David
Herrera-Kao, Wilberth Antonio
May-Pat, Alejandro
Ávila-Ortega, Alejandro
Rodríguez-Fuentes, Nayeli
Uribe-Calderón, Jorge Alonso
Cervantes-Uc, José Manuel
author_facet Abreu-Rejón, Antonio David
Herrera-Kao, Wilberth Antonio
May-Pat, Alejandro
Ávila-Ortega, Alejandro
Rodríguez-Fuentes, Nayeli
Uribe-Calderón, Jorge Alonso
Cervantes-Uc, José Manuel
author_sort Abreu-Rejón, Antonio David
collection PubMed
description Grafting polyethylene glycol (PEG) onto a polymer’s surface is widely used to improve biocompatibility by reducing protein and cell adhesion. Although PEG is considered to be bioinert, its incorporation onto biomaterials has shown to improve cell viability depending on the amount and molecular weight (MW) used. This phenomenon was studied here by grafting PEG of three MW onto polyurethane (PU) substrata at three molar concentrations to assess their effect on PU surface properties and on the viability of osteoblasts and fibroblasts. PEG formed a covering on the substrata which increased the hydrophilicity and surface energy of PUs. Among the results, it was observed that osteoblast viability increased for all MW and grafting densities of PEG employed compared with unmodified PU. However, fibroblast viability only increased at certain combinations of MW and grafting densities of PEG, suggesting an optimal level of these parameters. PEG grafting also promoted a more spread cell morphology than that exhibited by unmodified PU; nevertheless, cells became apoptotic-like as PEG MW and grafting density were increased. These effects on cells could be due to PEG affecting culture medium pH, which became more alkaline at higher MW and concentrations of PEG. Results support the hypothesis that surface energy of PU substrates can be tuned by controlling the MW and grafting density of PEG, but these parameters should be optimized to promote cell viability without inducing apoptotic-like behavior.
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spelling pubmed-96981722022-11-26 Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts Abreu-Rejón, Antonio David Herrera-Kao, Wilberth Antonio May-Pat, Alejandro Ávila-Ortega, Alejandro Rodríguez-Fuentes, Nayeli Uribe-Calderón, Jorge Alonso Cervantes-Uc, José Manuel Polymers (Basel) Article Grafting polyethylene glycol (PEG) onto a polymer’s surface is widely used to improve biocompatibility by reducing protein and cell adhesion. Although PEG is considered to be bioinert, its incorporation onto biomaterials has shown to improve cell viability depending on the amount and molecular weight (MW) used. This phenomenon was studied here by grafting PEG of three MW onto polyurethane (PU) substrata at three molar concentrations to assess their effect on PU surface properties and on the viability of osteoblasts and fibroblasts. PEG formed a covering on the substrata which increased the hydrophilicity and surface energy of PUs. Among the results, it was observed that osteoblast viability increased for all MW and grafting densities of PEG employed compared with unmodified PU. However, fibroblast viability only increased at certain combinations of MW and grafting densities of PEG, suggesting an optimal level of these parameters. PEG grafting also promoted a more spread cell morphology than that exhibited by unmodified PU; nevertheless, cells became apoptotic-like as PEG MW and grafting density were increased. These effects on cells could be due to PEG affecting culture medium pH, which became more alkaline at higher MW and concentrations of PEG. Results support the hypothesis that surface energy of PU substrates can be tuned by controlling the MW and grafting density of PEG, but these parameters should be optimized to promote cell viability without inducing apoptotic-like behavior. MDPI 2022-11-14 /pmc/articles/PMC9698172/ /pubmed/36433040 http://dx.doi.org/10.3390/polym14224912 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abreu-Rejón, Antonio David
Herrera-Kao, Wilberth Antonio
May-Pat, Alejandro
Ávila-Ortega, Alejandro
Rodríguez-Fuentes, Nayeli
Uribe-Calderón, Jorge Alonso
Cervantes-Uc, José Manuel
Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts
title Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts
title_full Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts
title_fullStr Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts
title_full_unstemmed Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts
title_short Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts
title_sort influence of molecular weight and grafting density of peg on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698172/
https://www.ncbi.nlm.nih.gov/pubmed/36433040
http://dx.doi.org/10.3390/polym14224912
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